1wq3

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(New page: 200px<br /><applet load="1wq3" size="450" color="white" frame="true" align="right" spinBox="true" caption="1wq3, resolution 2.00&Aring;" /> '''Escherichia coli tyr...)
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[[Image:1wq3.gif|left|200px]]<br /><applet load="1wq3" size="450" color="white" frame="true" align="right" spinBox="true"
 
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caption="1wq3, resolution 2.00&Aring;" />
 
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'''Escherichia coli tyrosyl-tRNA synthetase mutant comlexed with 3-iodo-L-tyrosine'''<br />
 
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==Overview==
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==Escherichia coli tyrosyl-tRNA synthetase mutant complexed with 3-iodo-L-tyrosine==
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The genetic code in a eukaryotic system has been expanded by the, engineering of Escherichia coli tyrosyl-tRNA synthetase (TyrRS) with the, Y37V and Q195C mutations (37V195C), which specifically recognize, 3-iodo-L-tyrosine rather than L-tyrosine. In the present study, we, determined the 3-iodo-L-tyrosine- and L-tyrosine-bound structures of the, 37V195C mutant of the E. coli TyrRS catalytic domain at 2.0-A resolution., The gamma-methyl group of Val-37 and the sulfur atom of Cys-195 make van, der Waals contacts with the iodine atom of 3-iodo-L-tyrosine. The Val-37, and Cys-195 side chains are rigidly fixed by the neighboring residues, forming the hydrophobic core of the TyrRS. The major roles of the two, mutations are different for the 3-iodo-L-tyrosine-selective recognition in, the first step of the aminoacylation reaction (the amino acid activation, step): the Y37V mutation eliminates the fatal steric repulsion with the, iodine atom, and the Q195C mutation reduces the L-tyrosine misrecognition., The structure of the 37V195C mutant TyrRS complexed with an, L-tyrosyladenylate analogue was also solved, indicating that the, 3-iodo-L-tyrosine and L-tyrosine side chains are similarly discriminated, in the second step (the aminoacyl transfer step). These results, demonstrate that the amino acid-binding pocket on the 37V195C mutant is, optimized for specific 3-iodo-L-tyrosine recognition.
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<StructureSection load='1wq3' size='340' side='right'caption='[[1wq3]], [[Resolution|resolution]] 2.00&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[1wq3]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli_str._K-12_substr._W3110 Escherichia coli str. K-12 substr. W3110]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1WQ3 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1WQ3 FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=IYR:3-IODO-TYROSINE'>IYR</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1wq3 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1wq3 OCA], [https://pdbe.org/1wq3 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1wq3 RCSB], [https://www.ebi.ac.uk/pdbsum/1wq3 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1wq3 ProSAT], [https://www.topsan.org/Proteins/RSGI/1wq3 TOPSAN]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/SYY_ECOLI SYY_ECOLI] Catalyzes the attachment of tyrosine to tRNA(Tyr) in a two-step reaction: tyrosine is first activated by ATP to form Tyr-AMP and then transferred to the acceptor end of tRNA(Tyr).[HAMAP-Rule:MF_02006]
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/wq/1wq3_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1wq3 ConSurf].
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<div style="clear:both"></div>
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==About this Structure==
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==See Also==
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1WQ3 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli] with IYR as [http://en.wikipedia.org/wiki/ligand ligand]. Active as [http://en.wikipedia.org/wiki/Tyrosine--tRNA_ligase Tyrosine--tRNA ligase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=6.1.1.1 6.1.1.1] Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1WQ3 OCA].
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*[[Aminoacyl tRNA synthetase 3D structures|Aminoacyl tRNA synthetase 3D structures]]
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__TOC__
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==Reference==
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</StructureSection>
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Structural basis of nonnatural amino acid recognition by an engineered aminoacyl-tRNA synthetase for genetic code expansion., Kobayashi T, Sakamoto K, Takimura T, Sekine R, Kelly VP, Kamata K, Nishimura S, Yokoyama S, Proc Natl Acad Sci U S A. 2005 Feb 1;102(5):1366-71. Epub 2005 Jan 25. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=15671170 15671170]
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[[Category: Escherichia coli str. K-12 substr. W3110]]
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[[Category: Escherichia coli]]
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[[Category: Large Structures]]
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[[Category: Single protein]]
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[[Category: Kamata K]]
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[[Category: Tyrosine--tRNA ligase]]
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[[Category: Kobayashi T]]
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[[Category: Kamata, K.]]
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[[Category: Nishimura S]]
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[[Category: Kobayashi, T.]]
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[[Category: Nureki O]]
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[[Category: Nishimura, S.]]
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[[Category: Sakamoto K]]
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[[Category: Nureki, O.]]
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[[Category: Sekine R]]
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[[Category: RSGI, RIKEN.Structural.Genomics/Proteomics.Initiative.]]
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[[Category: Takimura T]]
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[[Category: Sakamoto, K.]]
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[[Category: Yokoyama S]]
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[[Category: Sekine, R.]]
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[[Category: Takimura, T.]]
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[[Category: Yokoyama, S.]]
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[[Category: IYR]]
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[[Category: aminoacyl-trna sybthetase]]
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[[Category: ligase]]
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[[Category: protein-3-iodotyrosine complex]]
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[[Category: riken structural genomics/proteomics initiative]]
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[[Category: rsgi]]
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[[Category: structural genomics]]
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''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Wed Nov 21 05:35:57 2007''
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Current revision

Escherichia coli tyrosyl-tRNA synthetase mutant complexed with 3-iodo-L-tyrosine

PDB ID 1wq3

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