2jlj

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{{Seed}}
 
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[[Image:2jlj.jpg|left|200px]]
 
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==Crystal Structure of the cytoplasmic domain of Yersinia pestis YscU N263A P264A mutant==
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The line below this paragraph, containing "STRUCTURE_2jlj", creates the "Structure Box" on the page.
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<StructureSection load='2jlj' size='340' side='right'caption='[[2jlj]], [[Resolution|resolution]] 1.30&Aring;' scene=''>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>[[2jlj]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Yersinia_pestis Yersinia pestis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2JLJ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2JLJ FirstGlance]. <br>
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or leave the SCENE parameter empty for the default display.
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.3&#8491;</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2jlj FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2jlj OCA], [https://pdbe.org/2jlj PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2jlj RCSB], [https://www.ebi.ac.uk/pdbsum/2jlj PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2jlj ProSAT]</span></td></tr>
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{{STRUCTURE_2jlj| PDB=2jlj | SCENE= }}
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/YSCU_YERPE YSCU_YERPE] Component of the yop secretion machinery (By similarity).
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/jl/2jlj_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2jlj ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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Crystal structures of cleaved and uncleaved forms of the YscU cytoplasmic domain, an essential component of the type III secretion system (T3SS) in Yersinia pestis, have been solved by single-wavelength anomolous dispersion and refined with X-ray diffraction data extending up to atomic resolution (1.13 A). These crystallographic studies provide structural insights into the conformational changes induced upon auto-cleavage of the cytoplasmic domain of YscU. The structures indicate that the cleaved fragments remain bound to each other. The conserved NPTH sequence that contains the site of the N263-P264 peptide bond cleavage is found on a beta-turn which, upon cleavage, undergoes a major reorientation of the loop away from the catalytic N263, resulting in altered electrostatic surface features at the site of cleavage. Additionally, a significant conformational change was observed in the N-terminal linker regions of the cleaved and noncleaved forms of YscU which may correspond to the molecular switch that influences substrate specificity. The YscU structures determined here also are in good agreement with the auto-cleavage mechanism described for the flagellar homolog FlhB and E. coli EscU.
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===CRYSTAL STRUCTURE OF THE CYTOPLASMIC DOMAIN OF YERSINIA PESTIS YSCU N263A P264A MUTANT===
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Atomic resolution structure of the cytoplasmic domain of Yersinia pestis YscU, a regulatory switch involved in type III secretion.,Lountos GT, Austin BP, Nallamsetty S, Waugh DS Protein Sci. 2009 Jan 6;18(2):467-474. PMID:19165725<ref>PMID:19165725</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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The line below this paragraph, {{ABSTRACT_PUBMED_19165725}}, adds the Publication Abstract to the page
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<div class="pdbe-citations 2jlj" style="background-color:#fffaf0;"></div>
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(as it appears on PubMed at http://www.pubmed.gov), where 19165725 is the PubMed ID number.
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== References ==
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<references/>
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{{ABSTRACT_PUBMED_19165725}}
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__TOC__
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</StructureSection>
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==About this Structure==
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[[Category: Large Structures]]
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2JLJ is a 1 chain structure of sequence from [http://en.wikipedia.org/wiki/Yersinia_pestis Yersinia pestis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2JLJ OCA].
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==Reference==
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<ref group="xtra">PMID:19165725</ref><references group="xtra"/>
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[[Category: Yersinia pestis]]
[[Category: Yersinia pestis]]
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[[Category: Austin, B P.]]
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[[Category: Austin BP]]
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[[Category: Lountos, G T.]]
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[[Category: Lountos GT]]
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[[Category: Nallamsetty, S.]]
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[[Category: Nallamsetty S]]
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[[Category: Waugh, D S.]]
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[[Category: Waugh DS]]
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[[Category: Cell membrane]]
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[[Category: Membrane]]
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[[Category: Plasmid]]
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[[Category: Protein transport]]
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[[Category: Transmembrane]]
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[[Category: Transport]]
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[[Category: Type iii secretion system]]
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[[Category: Virulence]]
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[[Category: Yersinia pesit]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Feb 4 11:11:55 2009''
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Current revision

Crystal Structure of the cytoplasmic domain of Yersinia pestis YscU N263A P264A mutant

PDB ID 2jlj

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