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1wri

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(New page: 200px<br /><applet load="1wri" size="450" color="white" frame="true" align="right" spinBox="true" caption="1wri, resolution 1.20&Aring;" /> '''Crystal Structure of...)
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[[Image:1wri.jpg|left|200px]]<br /><applet load="1wri" size="450" color="white" frame="true" align="right" spinBox="true"
 
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caption="1wri, resolution 1.20&Aring;" />
 
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'''Crystal Structure of Ferredoxin isoform II from E. arvense'''<br />
 
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==Overview==
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==Crystal Structure of Ferredoxin isoform II from E. arvense==
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We have determined the crystal structure, at 1.2-A resolution, of, Equisetum arvense ferredoxin isoform II (FdII), which lacks residues, equivalent to Arg(39) and Glu(28) highly conserved among other ferredoxins, (Fds). In other Fds these residues form an intramolecular salt bridge, crucial for stabilization of the [2Fe-2S] cluster, which is disrupted upon, complex formation with Fd-NADP(+) oxidoreductase (FNR) to form two, intermolecular salt bridges. The overall structure of FdII resembles the, known backbone structures of E. arvense isoform I (FdI) and other, plant-type Fds. Dramatically, in the FdII structure a unique, alternative, salt bridge is formed between Arg(22) and Glu(58). This results in a, different relative orientation of the alpha-helix formed by, Leu(23)-Glu(29) and eliminates the possibility of forming three of the, five intermolecular salt bridges identified on formation of a complex, between maize FdI and maize FNR. Mutation of FdII, informed by structural, differences with FdI, showed that the alternative salt bridge and the, absence of an otherwise conserved Tyr residue are important for the, alternative stabilization of the FdII [2Fe-2S] cluster. We also, investigated FdI and FdII electron transfer to FNR on chloroplast, thylakoid membranes. The K(m) and V(max) values of FdII are similar to, those of FdI, contrary to previous measurements of the reverse reaction, from FNR to Fd. The affinity between reduced FdI and oxidized FNR is much, greater than that between oxidized FdI and reduced FNR, whereas this is, not the case with FdII. The pH dependence of electron transfer by FdI, FdII, and an FdII mutant with FdI features was measured and further, indicated that the binding mode to FNR differs between FdI and FdII. Based, on this evidence, we hypothesize that binding modes with other, Fd-dependent reductases may also vary between FdI and FdII. The structural, differences between FdI and FdII therefore result in functional, differences that may influence partitioning of electrons into different, redox metabolic pathways.
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<StructureSection load='1wri' size='340' side='right'caption='[[1wri]], [[Resolution|resolution]] 1.20&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[1wri]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Equisetum_arvense Equisetum arvense]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1WRI OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1WRI FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.2&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=BEN:BENZAMIDINE'>BEN</scene>, <scene name='pdbligand=FES:FE2/S2+(INORGANIC)+CLUSTER'>FES</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1wri FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1wri OCA], [https://pdbe.org/1wri PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1wri RCSB], [https://www.ebi.ac.uk/pdbsum/1wri PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1wri ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/FER2_EQUAR FER2_EQUAR] Ferredoxins are iron-sulfur proteins that transfer electrons in a wide variety of metabolic reactions.
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/wr/1wri_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1wri ConSurf].
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<div style="clear:both"></div>
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==About this Structure==
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==See Also==
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1WRI is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Equisetum_arvense Equisetum arvense] with FES and BEN as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1WRI OCA].
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*[[Ferredoxin 3D structures|Ferredoxin 3D structures]]
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__TOC__
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==Reference==
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</StructureSection>
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A structural basis of Equisetum arvense ferredoxin isoform II producing an alternative electron transfer with ferredoxin-NADP+ reductase., Kurisu G, Nishiyama D, Kusunoki M, Fujikawa S, Katoh M, Hanke GT, Hase T, Teshima K, J Biol Chem. 2005 Jan 21;280(3):2275-81. Epub 2004 Oct 28. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=15513928 15513928]
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[[Category: Equisetum arvense]]
[[Category: Equisetum arvense]]
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[[Category: Single protein]]
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[[Category: Large Structures]]
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[[Category: Fujikawa, S.]]
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[[Category: Fujikawa S]]
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[[Category: Hanke, G.T.]]
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[[Category: Hanke GT]]
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[[Category: Hase, T.]]
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[[Category: Hase T]]
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[[Category: Katoh, M.]]
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[[Category: Katoh M]]
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[[Category: Kurisu, G.]]
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[[Category: Kurisu G]]
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[[Category: Kusunoki, M.]]
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[[Category: Kusunoki M]]
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[[Category: Nishiyama, D.]]
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[[Category: Nishiyama D]]
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[[Category: Teshima, K.]]
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[[Category: Teshima K]]
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[[Category: BEN]]
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[[Category: FES]]
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[[Category: electron transport]]
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''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Wed Nov 21 05:37:21 2007''
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Current revision

Crystal Structure of Ferredoxin isoform II from E. arvense

PDB ID 1wri

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