2w40

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{{Seed}}
 
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[[Image:2w40.png|left|200px]]
 
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==Crystal structure of Plasmodium falciparum glycerol kinase with bound glycerol==
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The line below this paragraph, containing "STRUCTURE_2w40", creates the "Structure Box" on the page.
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<StructureSection load='2w40' size='340' side='right'caption='[[2w40]], [[Resolution|resolution]] 1.49&Aring;' scene=''>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>[[2w40]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Plasmodium_falciparum_3D7 Plasmodium falciparum 3D7]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2W40 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2W40 FirstGlance]. <br>
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or leave the SCENE parameter empty for the default display.
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.49&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene></td></tr>
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{{STRUCTURE_2w40| PDB=2w40 | SCENE= }}
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2w40 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2w40 OCA], [https://pdbe.org/2w40 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2w40 RCSB], [https://www.ebi.ac.uk/pdbsum/2w40 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2w40 ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/Q8IDI4_PLAF7 Q8IDI4_PLAF7]
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/w4/2w40_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2w40 ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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Summary Malaria pathology is caused by multiplication of asexual parasites within erythrocytes, whereas mosquito transmission of malaria is mediated by sexual precursor cells (gametocytes). Microarray analysis identified glycerol kinase (GK) as the second most highly upregulated gene in Plasmodium falciparum gametocytes with no expression detectable in asexual blood stage parasites. Phosphorylation of glycerol by GK is the rate-limiting step in glycerol utilisation. Deletion of this gene from P. falciparum had no effect on asexual parasite growth, but surprisingly also had no effect on gametocyte development or exflagellation suggesting that these life cycle stages do not utilise host-derived glycerol as a carbon source. Kinetic studies of purified PfGK showed that the enzyme is not regulated by fructose 1,6 bisphosphate. The high-resolution crystal structure of P. falciparum glycerol kinase, the first of a eukaryotic GK, reveals two domains embracing a capacious ligand-binding groove. In the complexes of PfGK with glycerol and ADP, we observed closed and open forms of the active site, respectively. The 27 degrees domain opening is larger than in orthologous systems and exposes an extensive surface with potential for exploitation in selective inhibitor design should the enzyme prove to be essential in vivo either in the human or in the mosquito.
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===CRYSTAL STRUCTURE OF PLASMODIUM FALCIPARUM GLYCEROL KINASE WITH BOUND GLYCEROL===
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Structure and non-essential function of glycerol kinase in Plasmodium falciparum blood stages.,Schnick C, Polley SD, Fivelman QL, Ranford-Cartwright LC, Wilkinson SR, Brannigan JA, Wilkinson AJ, Baker DA Mol Microbiol. 2008 Nov 24. PMID:19040641<ref>PMID:19040641</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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The line below this paragraph, {{ABSTRACT_PUBMED_19040641}}, adds the Publication Abstract to the page
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<div class="pdbe-citations 2w40" style="background-color:#fffaf0;"></div>
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(as it appears on PubMed at http://www.pubmed.gov), where 19040641 is the PubMed ID number.
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== References ==
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<references/>
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{{ABSTRACT_PUBMED_19040641}}
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__TOC__
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</StructureSection>
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==About this Structure==
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[[Category: Large Structures]]
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2W40 is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Plasmodium_falciparum Plasmodium falciparum]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2W40 OCA].
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[[Category: Plasmodium falciparum 3D7]]
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[[Category: Baker DA]]
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==Reference==
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[[Category: Brannigan JA]]
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Structure and non-essential function of glycerol kinase in Plasmodium falciparum blood stages., Schnick C, Polley SD, Fivelman QL, Ranford-Cartwright LC, Wilkinson SR, Brannigan JA, Wilkinson AJ, Baker DA, Mol Microbiol. 2008 Nov 24. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/19040641 19040641]
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[[Category: Fivelman QL]]
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[[Category: Glycerol kinase]]
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[[Category: Polley SD]]
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[[Category: Plasmodium falciparum]]
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[[Category: Ranford-Cartwright L]]
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[[Category: Single protein]]
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[[Category: Schnick C]]
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[[Category: Pdbx_ordinal=, <PDBx:audit_author.]]
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[[Category: Wilkinson AJ]]
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[[Category: Closed conformation]]
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[[Category: Wilkinson SR]]
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[[Category: Glycerol kinase]]
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[[Category: Kinase]]
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[[Category: Malaria]]
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[[Category: Open conformation]]
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[[Category: Plasmodium]]
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[[Category: Sugar kinase/hsp70/actin superfamily]]
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[[Category: Transferase]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Feb 4 20:21:11 2009''
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Current revision

Crystal structure of Plasmodium falciparum glycerol kinase with bound glycerol

PDB ID 2w40

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