1wwl

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(New page: 200px<br /><applet load="1wwl" size="450" color="white" frame="true" align="right" spinBox="true" caption="1wwl, resolution 2.50&Aring;" /> '''Crystal structure of...)
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[[Image:1wwl.gif|left|200px]]<br /><applet load="1wwl" size="450" color="white" frame="true" align="right" spinBox="true"
 
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caption="1wwl, resolution 2.50&Aring;" />
 
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'''Crystal structure of CD14'''<br />
 
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==Overview==
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==Crystal structure of CD14==
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Lipopolysaccharide, the endotoxin of Gram-negative bacteria, induces, extensive immune responses that can lead to fatal septic shock syndrome., The core receptors recognizing lipopolysaccharide are CD14, TLR4, and, MD-2. CD14 binds to lipopolysaccharide and presents it to the TLR4/MD-2, complex, which initiates intracellular signaling. In addition to, lipopolysaccharide, CD14 is capable of recognizing a few other microbial, and cellular products. Here, we present the first crystal structure of, CD14 to 2.5 angstroms resolution. A large hydrophobic pocket was found on, the NH2-terminal side of the horseshoe-like structure. Previously, identified regions involved in lipopolysaccharide binding map to the rim, and bottom of the pocket indicating that the pocket is the main component, of the lipopolysaccharide-binding site. Mutations that interfere with, lipopolysaccharide signaling but not with lipopolysaccharide binding are, also clustered in a separate area near the pocket. Ligand diversity of, CD14 could be explained by the generous size of the pocket, the, considerable flexibility of the rim of the pocket, and the multiplicity of, grooves available for ligand binding.
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<StructureSection load='1wwl' size='340' side='right'caption='[[1wwl]], [[Resolution|resolution]] 2.50&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[1wwl]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1WWL OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1WWL FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.5&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1wwl FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1wwl OCA], [https://pdbe.org/1wwl PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1wwl RCSB], [https://www.ebi.ac.uk/pdbsum/1wwl PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1wwl ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/CD14_MOUSE CD14_MOUSE] Cooperates with MD-2 and TLR4 to mediate the innate immune response to bacterial lipopolysaccharide (LPS). Acts via MyD88, TIRAP and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response. Up-regulates cell surface molecules, including adhesion molecules (By similarity).
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ww/1wwl_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview03.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1wwl ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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Lipopolysaccharide, the endotoxin of Gram-negative bacteria, induces extensive immune responses that can lead to fatal septic shock syndrome. The core receptors recognizing lipopolysaccharide are CD14, TLR4, and MD-2. CD14 binds to lipopolysaccharide and presents it to the TLR4/MD-2 complex, which initiates intracellular signaling. In addition to lipopolysaccharide, CD14 is capable of recognizing a few other microbial and cellular products. Here, we present the first crystal structure of CD14 to 2.5 angstroms resolution. A large hydrophobic pocket was found on the NH2-terminal side of the horseshoe-like structure. Previously identified regions involved in lipopolysaccharide binding map to the rim and bottom of the pocket indicating that the pocket is the main component of the lipopolysaccharide-binding site. Mutations that interfere with lipopolysaccharide signaling but not with lipopolysaccharide binding are also clustered in a separate area near the pocket. Ligand diversity of CD14 could be explained by the generous size of the pocket, the considerable flexibility of the rim of the pocket, and the multiplicity of grooves available for ligand binding.
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==About this Structure==
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Crystal structure of CD14 and its implications for lipopolysaccharide signaling.,Kim JI, Lee CJ, Jin MS, Lee CH, Paik SG, Lee H, Lee JO J Biol Chem. 2005 Mar 25;280(12):11347-51. Epub 2005 Jan 10. PMID:15644310<ref>PMID:15644310</ref>
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1WWL is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Mus_musculus Mus musculus] with NAG as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1WWL OCA].
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==Reference==
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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Crystal structure of CD14 and its implications for lipopolysaccharide signaling., Kim JI, Lee CJ, Jin MS, Lee CH, Paik SG, Lee H, Lee JO, J Biol Chem. 2005 Mar 25;280(12):11347-51. Epub 2005 Jan 10. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=15644310 15644310]
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</div>
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<div class="pdbe-citations 1wwl" style="background-color:#fffaf0;"></div>
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== References ==
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<references/>
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__TOC__
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</StructureSection>
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[[Category: Large Structures]]
[[Category: Mus musculus]]
[[Category: Mus musculus]]
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[[Category: Single protein]]
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[[Category: Jin MS]]
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[[Category: Jin, M.S.]]
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[[Category: Kim J-I]]
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[[Category: Kim, J.I.]]
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[[Category: Lee C-H]]
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[[Category: Lee, C.H.]]
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[[Category: Lee CJ]]
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[[Category: Lee, C.J.]]
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[[Category: Lee H]]
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[[Category: Lee, H.]]
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[[Category: Lee J-O]]
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[[Category: Lee, J.O.]]
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[[Category: Paik S-G]]
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[[Category: Paik, S.G.]]
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[[Category: NAG]]
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[[Category: cd14]]
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[[Category: lps]]
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''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Wed Nov 21 05:43:16 2007''
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Current revision

Crystal structure of CD14

PDB ID 1wwl

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