3cm3

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[[Image:3cm3.jpg|left|200px]]
 
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==High Resolution Crystal Structure of the Vaccinia Virus Dual-Specificity Phosphatase VH1==
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The line below this paragraph, containing "STRUCTURE_3cm3", creates the "Structure Box" on the page.
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<StructureSection load='3cm3' size='340' side='right'caption='[[3cm3]], [[Resolution|resolution]] 1.32&Aring;' scene=''>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>[[3cm3]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Vaccinia_virus Vaccinia virus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3CM3 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3CM3 FirstGlance]. <br>
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or leave the SCENE parameter empty for the default display.
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.32&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=BME:BETA-MERCAPTOETHANOL'>BME</scene>, <scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene></td></tr>
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{{STRUCTURE_3cm3| PDB=3cm3 | SCENE= }}
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3cm3 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3cm3 OCA], [https://pdbe.org/3cm3 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3cm3 RCSB], [https://www.ebi.ac.uk/pdbsum/3cm3 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3cm3 ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/DUSP_VACCW DUSP_VACCW] Serine/Tyrosine phosphatase which down-regulates cellular antiviral response by dephosphorylating activated host STAT1 and blocking interferon (IFN)-stimulated innate immune responses. Dephosphorylates the A17 protein.<ref>PMID:10438817</ref> <ref>PMID:11238845</ref> <ref>PMID:1848923</ref> <ref>PMID:18593332</ref> <ref>PMID:21362620</ref>
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/cm/3cm3_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=3cm3 ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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The Vaccinia virus H1 gene product, VH1, is a dual specificity phosphatase that down-regulates the cellular antiviral response by dephosphorylating STAT1. The crystal structure of VH1, determined at 1.32 A resolution, reveals a novel dimeric quaternary structure, which exposes two active sites spaced approximately 39 A away from each other. VH1 forms a stable dimer via an extensive domain swap of the N-terminal helix (residues 1-20). In vitro, VH1 can dephosphorylate activated STAT1, in a reaction that is competed by the nuclear transport adapter importin alpha5. Interestingly, VH1 is inactive with respect to STAT1 bound to DNA, suggesting that the viral phosphatase acts predominantly on the cytoplasmic pool of activated STAT1. We propose that the dimeric quaternary structure of VH1 is essential for specific recognition of activated STAT1, which prevents its nuclear translocation, thus blocking interferon-gamma signal transduction and antiviral response.
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===High Resolution Crystal Structure of the Vaccinia Virus Dual-Specificity Phosphatase VH1===
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Dimeric quaternary structure of the prototypical dual specificity phosphatase VH1.,Koksal AC, Nardozzi JD, Cingolani G J Biol Chem. 2009 Apr 10;284(15):10129-37. Epub 2009 Feb 10. PMID:19211553<ref>PMID:19211553</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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<div class="pdbe-citations 3cm3" style="background-color:#fffaf0;"></div>
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==About this Structure==
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==See Also==
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3CM3 is a 1 chain structure of sequence from [http://en.wikipedia.org/wiki/Vaccinia_virus Vaccinia virus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3CM3 OCA].
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*[[Dual specificity phosphatase 3D structures|Dual specificity phosphatase 3D structures]]
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== References ==
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<references/>
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__TOC__
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</StructureSection>
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[[Category: Large Structures]]
[[Category: Vaccinia virus]]
[[Category: Vaccinia virus]]
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[[Category: Cingolani, G.]]
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[[Category: Cingolani G]]
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[[Category: Koksal, A C.]]
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[[Category: Koksal AC]]
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[[Category: Dual-specificity phosphatase]]
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[[Category: Hydrolase]]
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[[Category: Late protein]]
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[[Category: Protein phosphatase]]
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[[Category: Vaccinia virus]]
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[[Category: Vh1]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Feb 11 12:39:56 2009''
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Current revision

High Resolution Crystal Structure of the Vaccinia Virus Dual-Specificity Phosphatase VH1

PDB ID 3cm3

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