1wz3

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(New page: 200px<br /><applet load="1wz3" size="450" color="white" frame="true" align="right" spinBox="true" caption="1wz3, resolution 1.80&Aring;" /> '''The crystal structur...)
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[[Image:1wz3.gif|left|200px]]<br /><applet load="1wz3" size="450" color="white" frame="true" align="right" spinBox="true"
 
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caption="1wz3, resolution 1.80&Aring;" />
 
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'''The crystal structure of plant ATG12'''<br />
 
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==Overview==
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==The crystal structure of plant ATG12==
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Atg12 is a post-translational modifier that is activated and conjugated to, its single target, Atg5, by a ubiquitin-like conjugation system. The, Atg12-Atg5 conjugate is essential for autophagy, the bulk degradation, process of cytoplasmic components by the vacuolar/lysosomal system. Here, we demonstrate that the Atg12 conjugation system exists in Arabidopsis and, is essential for plant autophagy as well as in yeast and mammals. We also, report the crystal structure of Arabidopsis thaliana (At) ATG12 at 1.8 A, resolution. Despite no obvious sequence homology with ubiquitin, the, structure of AtATG12 shows a ubiquitin fold strikingly similar to those of, mammalian homologs of Atg8, the other ubiquitin-like modifier essential, for autophagy, which is conjugated to phosphatidylethanolamine. Two types, of hydrophobic patches are present on the surface of AtATG12: one is, conserved in both Atg12 and Atg8 orthologs, while the other is unique to, Atg12 orthologs. Considering that they share Atg7 as an E1-like enzyme, we, suggest that the first hydrophobic patch is responsible for the, conjugation reaction, while the latter is involved in Atg12-specific, functions.
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<StructureSection load='1wz3' size='340' side='right'caption='[[1wz3]], [[Resolution|resolution]] 1.80&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[1wz3]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Arabidopsis_thaliana Arabidopsis thaliana]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1WZ3 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1WZ3 FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.8&#8491;</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1wz3 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1wz3 OCA], [https://pdbe.org/1wz3 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1wz3 RCSB], [https://www.ebi.ac.uk/pdbsum/1wz3 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1wz3 ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/AT12B_ARATH AT12B_ARATH] Ubiquitin-like protein involved in cytoplasm to vacuole transport (Cvt) and autophagy vesicles formation. Conjugation with ATG5 through a ubiquitin-like conjugating system involving also ATG7 as an E1-like activating enzyme and ATG10 as an E2-like conjugating enzyme, is essential for its function. ATG12/ATG5 conjugate has an essential role in plant nutrient recycling.
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/wz/1wz3_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1wz3 ConSurf].
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<div style="clear:both"></div>
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==About this Structure==
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==See Also==
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1WZ3 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Arabidopsis_thaliana Arabidopsis thaliana]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1WZ3 OCA].
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*[[Autophagy-related protein 3D structures|Autophagy-related protein 3D structures]]
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__TOC__
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==Reference==
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</StructureSection>
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The crystal structure of plant ATG12 and its biological implication in autophagy., Suzuki NN, Yoshimoto K, Fujioka Y, Ohsumi Y, Inagaki F, Autophagy. 2005 Jul;1(2):119-26. Epub 2005 Jul 23. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=16874047 16874047]
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[[Category: Arabidopsis thaliana]]
[[Category: Arabidopsis thaliana]]
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[[Category: Single protein]]
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[[Category: Large Structures]]
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[[Category: Fujioka, Y.]]
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[[Category: Fujioka Y]]
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[[Category: Inagaki, F.]]
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[[Category: Inagaki F]]
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[[Category: Ohsumi, Y.]]
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[[Category: Ohsumi Y]]
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[[Category: Suzuki, N.N.]]
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[[Category: Suzuki NN]]
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[[Category: Yoshimoto, K.]]
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[[Category: Yoshimoto K]]
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[[Category: ubiquitin-fold]]
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''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Wed Nov 21 05:45:28 2007''
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Current revision

The crystal structure of plant ATG12

PDB ID 1wz3

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