3fgp

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{{Seed}}
 
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[[Image:3fgp.png|left|200px]]
 
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==2.05 a Crystal Structure of CysM from Mycobacterium Tuberculosis - Open and Closed Conformations==
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The line below this paragraph, containing "STRUCTURE_3fgp", creates the "Structure Box" on the page.
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<StructureSection load='3fgp' size='340' side='right'caption='[[3fgp]], [[Resolution|resolution]] 2.05&Aring;' scene=''>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>[[3fgp]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Mycobacterium_tuberculosis Mycobacterium tuberculosis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3FGP OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3FGP FirstGlance]. <br>
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or leave the SCENE parameter empty for the default display.
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.05&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=LLP:(2S)-2-AMINO-6-[[3-HYDROXY-2-METHYL-5-(PHOSPHONOOXYMETHYL)PYRIDIN-4-YL]METHYLIDENEAMINO]HEXANOIC+ACID'>LLP</scene></td></tr>
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{{STRUCTURE_3fgp| PDB=3fgp | SCENE= }}
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3fgp FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3fgp OCA], [https://pdbe.org/3fgp PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3fgp RCSB], [https://www.ebi.ac.uk/pdbsum/3fgp PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3fgp ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/CYSM_MYCTU CYSM_MYCTU] Catalyzes the formation of a covalent CysO-cysteine adduct via a sulfur transfer, using the thiocarboxylated sulfur carrier protein CysO-COSH as sulfur donor and O-phospho-L-serine (OPS) as sulfur acceptor. Can also use sodium sulfide as sulfur donor in vitro, albeit with less efficiency, but not thiosulfate or thio-nitro-benzoate. O-acetylserine (OAS) is a very poor substrate in comparison with OPS. May be of particular importance for cysteine biosynthesis in the persistent phase of M.tuberculosis.<ref>PMID:18842002</ref> <ref>PMID:18799456</ref>
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/fg/3fgp_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=3fgp ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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A new crystal structure of the dimeric cysteine synthase CysM from Mycobacterium tuberculosis reveals an open and a closed conformation of the enzyme. In the closed conformation the five carboxy-terminal amino acid residues are inserted into the active site cleft. Removal of this segment results in a decreased lifetime of the alpha-aminoacrylate reaction intermediate, an increased sensitivity to oxidants such as hydrogen peroxide, and loss of substrate selectivity with respect to the sulfur carrier thiocarboxylated CysO. These results highlight features of CysM that might be of particular importance for cysteine biosynthesis under oxidative stress in M. tuberculosis.
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===2.05 a Crystal Structure of CysM from Mycobacterium Tuberculosis - Open and Closed Conformations===
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The C-terminal of CysM from Mycobacterium tuberculosis protects the aminoacrylate intermediate and is involved in sulfur donor selectivity.,Agren D, Schnell R, Schneider G FEBS Lett. 2009 Jan 22;583(2):330-6. Epub 2008 Dec 26. PMID:19101553<ref>PMID:19101553</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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The line below this paragraph, {{ABSTRACT_PUBMED_19101553}}, adds the Publication Abstract to the page
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<div class="pdbe-citations 3fgp" style="background-color:#fffaf0;"></div>
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(as it appears on PubMed at http://www.pubmed.gov), where 19101553 is the PubMed ID number.
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== References ==
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<references/>
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{{ABSTRACT_PUBMED_19101553}}
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__TOC__
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</StructureSection>
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==About this Structure==
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[[Category: Large Structures]]
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3FGP is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Mycobacterium_tuberculosis Mycobacterium tuberculosis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3FGP OCA].
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==Reference==
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The C-terminal of CysM from Mycobacterium tuberculosis protects the aminoacrylate intermediate and is involved in sulfur donor selectivity., Gren D, Schnell R, Schneider G, FEBS Lett. 2009 Jan 22;583(2):330-6. Epub 2008 Dec 26. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/19101553 19101553]
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[[Category: Mycobacterium tuberculosis]]
[[Category: Mycobacterium tuberculosis]]
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[[Category: O-phosphoserine sulfhydrylase]]
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[[Category: Agren D]]
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[[Category: Single protein]]
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[[Category: Schneider G]]
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[[Category: Pdbx_ordinal=, <PDBx:audit_author.]]
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[[Category: Schnell R]]
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[[Category: Amino-acid biosynthesis]]
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[[Category: Cysm]]
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[[Category: Cyso]]
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[[Category: Cysteine biosynthesis]]
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[[Category: Cysteine synthase]]
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[[Category: O-phosphoserine]]
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[[Category: Pyridoxal phosphate]]
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[[Category: Transferase]]
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[[Category: Tuberculosis]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Feb 11 20:04:50 2009''
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Current revision

2.05 a Crystal Structure of CysM from Mycobacterium Tuberculosis - Open and Closed Conformations

PDB ID 3fgp

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