2v1x

From Proteopedia

(Difference between revisions)
Jump to: navigation, search
Current revision (15:02, 13 December 2023) (edit) (undo)
 
(11 intermediate revisions not shown.)
Line 1: Line 1:
-
{{Seed}}
 
-
[[Image:2v1x.png|left|200px]]
 
-
<!--
+
==Crystal structure of human RECQ-like DNA helicase==
-
The line below this paragraph, containing "STRUCTURE_2v1x", creates the "Structure Box" on the page.
+
<StructureSection load='2v1x' size='340' side='right'caption='[[2v1x]], [[Resolution|resolution]] 2.00&Aring;' scene=''>
-
You may change the PDB parameter (which sets the PDB file loaded into the applet)
+
== Structural highlights ==
-
or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
+
<table><tr><td colspan='2'>[[2v1x]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2V1X OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2V1X FirstGlance]. <br>
-
or leave the SCENE parameter empty for the default display.
+
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2&#8491;</td></tr>
-
-->
+
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ADP:ADENOSINE-5-DIPHOSPHATE'>ADP</scene>, <scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
-
{{STRUCTURE_2v1x| PDB=2v1x | SCENE= }}
+
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2v1x FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2v1x OCA], [https://pdbe.org/2v1x PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2v1x RCSB], [https://www.ebi.ac.uk/pdbsum/2v1x PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2v1x ProSAT]</span></td></tr>
 +
</table>
 +
== Function ==
 +
[https://www.uniprot.org/uniprot/RECQ1_HUMAN RECQ1_HUMAN] DNA helicase that may play a role in the repair of DNA that is damaged by ultraviolet light or other mutagens. Exhibits a magnesium-dependent ATP-dependent DNA-helicase activity that unwinds single- and double-stranded DNA in a 3'-5' direction.<ref>PMID:15886194</ref> <ref>PMID:7961977</ref> <ref>PMID:8056767</ref>
 +
== Evolutionary Conservation ==
 +
[[Image:Consurf_key_small.gif|200px|right]]
 +
Check<jmol>
 +
<jmolCheckbox>
 +
<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/v1/2v1x_consurf.spt"</scriptWhenChecked>
 +
<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
 +
<text>to colour the structure by Evolutionary Conservation</text>
 +
</jmolCheckbox>
 +
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2v1x ConSurf].
 +
<div style="clear:both"></div>
 +
<div style="background-color:#fffaf0;">
 +
== Publication Abstract from PubMed ==
 +
RecQ-like helicases, which include 5 members in the human genome, are important in maintaining genome integrity. We present a crystal structure of a truncated form of the human RECQ1 protein with Mg-ADP. The truncated protein is active in DNA fork unwinding but lacks other activities of the full-length enzyme: disruption of Holliday junctions and DNA strand annealing. The structure of human RECQ1 resembles that of Escherichia coli RecQ, with some important differences. All structural domains are conserved, including the 2 RecA-like domains and the RecQ-specific zinc-binding and winged-helix (WH) domains. However, the WH domain is positioned at a different orientation from that of the E. coli enzyme. We identify a prominent beta-hairpin of the WH domain as essential for DNA strand separation, which may be analogous to DNA strand-separation features of other DNA helicases. This hairpin is significantly shorter in the E. coli enzyme and is not required for its helicase activity, suggesting that there are significant differences between the modes of action of RecQ family members.
-
===CRYSTAL STRUCTURE OF HUMAN RECQ-LIKE DNA HELICASE===
+
Structure of the human RECQ1 helicase reveals a putative strand-separation pin.,Pike AC, Shrestha B, Popuri V, Burgess-Brown N, Muzzolini L, Costantini S, Vindigni A, Gileadi O Proc Natl Acad Sci U S A. 2009 Jan 16. PMID:19151156<ref>PMID:19151156</ref>
 +
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
 +
</div>
 +
<div class="pdbe-citations 2v1x" style="background-color:#fffaf0;"></div>
-
<!--
+
==See Also==
-
The line below this paragraph, {{ABSTRACT_PUBMED_19151156}}, adds the Publication Abstract to the page
+
*[[Helicase 3D structures|Helicase 3D structures]]
-
(as it appears on PubMed at http://www.pubmed.gov), where 19151156 is the PubMed ID number.
+
== References ==
-
-->
+
<references/>
-
{{ABSTRACT_PUBMED_19151156}}
+
__TOC__
-
 
+
</StructureSection>
-
==About this Structure==
+
-
2V1X is a 2 chains structure of sequences from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2V1X OCA].
+
-
 
+
-
==Reference==
+
-
<ref group="xtra">PMID:19151156</ref><references group="xtra"/>
+
[[Category: Homo sapiens]]
[[Category: Homo sapiens]]
-
[[Category: Arrowsmith, C H.]]
+
[[Category: Large Structures]]
-
[[Category: Burgess-Brown, N.]]
+
[[Category: Arrowsmith CH]]
-
[[Category: Edwards, A.]]
+
[[Category: Burgess-Brown N]]
-
[[Category: Gileadi, O.]]
+
[[Category: Edwards A]]
-
[[Category: King, O.]]
+
[[Category: Gileadi O]]
-
[[Category: Pike, A C.W.]]
+
[[Category: King O]]
-
[[Category: Shrestha, B.]]
+
[[Category: Pike ACW]]
-
[[Category: Sundstrom, M.]]
+
[[Category: Shrestha B]]
-
[[Category: Ugochukwu, E.]]
+
[[Category: Sundstrom M]]
-
[[Category: Watt, S.]]
+
[[Category: Ugochukwu E]]
-
[[Category: Weigelt, J.]]
+
[[Category: Watt S]]
-
[[Category: Atp-binding]]
+
[[Category: Weigelt J]]
-
[[Category: Atpase]]
+
-
[[Category: Dna strand annealing]]
+
-
[[Category: Dna-binding]]
+
-
[[Category: Helicase]]
+
-
[[Category: Hydrolase]]
+
-
[[Category: Mismatch repair]]
+
-
[[Category: Nuclear protein]]
+
-
[[Category: Nucleotide-binding]]
+
-
[[Category: Polymorphism]]
+
-
 
+
-
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Feb 15 08:45:06 2009''
+

Current revision

Crystal structure of human RECQ-like DNA helicase

PDB ID 2v1x

Drag the structure with the mouse to rotate

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/2v1x"
Personal tools