1ddn

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{{Seed}}
 
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[[Image:1ddn.png|left|200px]]
 
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==DIPHTHERIA TOX REPRESSOR (C102D MUTANT)/TOX DNA OPERATOR COMPLEX==
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The line below this paragraph, containing "STRUCTURE_1ddn", creates the "Structure Box" on the page.
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<StructureSection load='1ddn' size='340' side='right'caption='[[1ddn]], [[Resolution|resolution]] 3.00&Aring;' scene=''>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>[[1ddn]] is a 6 chain structure with sequence from [https://en.wikipedia.org/wiki/Corynebacterium_diphtheriae Corynebacterium diphtheriae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1DDN OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1DDN FirstGlance]. <br>
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or leave the SCENE parameter empty for the default display.
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 3&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=NI:NICKEL+(II)+ION'>NI</scene></td></tr>
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{{STRUCTURE_1ddn| PDB=1ddn | SCENE= }}
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1ddn FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1ddn OCA], [https://pdbe.org/1ddn PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1ddn RCSB], [https://www.ebi.ac.uk/pdbsum/1ddn PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1ddn ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/DTXR_CORDI DTXR_CORDI] Iron-binding repressor of the dipheteria toxin gene expression. May serve as a global regulator of gene expression. Represses ripA under iron excess.
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/dd/1ddn_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1ddn ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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The virulent phenotype of the pathogenic bacterium Corynebacterium diphtheriae is conferred by diphtheria toxin, whose expression is an adaptive response to low concentrations of iron. The expression of the toxin gene (tox) is regulated by the repressor DtxR, which is activated by transition metal ions. X-ray crystal structures of DtxR with and without (apo-form) its coordinated transition metal ion have established the general architecture of the repressor, identified the location of the metal-binding sites, and revealed a metal-ion-triggered subunit-subunit 'caliper-like' conformational change. Here we report the three-dimensional crystal structure of the complex between a biologically active Ni(II)-bound DtxR(C102D) mutant, in which a cysteine is replaced by an aspartate at residue 102, and a 33-base-pair DNA segment containing the toxin operator toxO. This structure shows that DNA interacts with two dimeric repressor proteins bound to opposite sides of the tox operator. We propose that a metal-ion-induced helix-to-coil structural transition in the amino-terminal region of the protein is partly responsible for the unique mode of repressor activation by transition metal ions.
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===DIPHTHERIA TOX REPRESSOR (C102D MUTANT)/TOX DNA OPERATOR COMPLEX===
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Structure of the metal-ion-activated diphtheria toxin repressor/tox operator complex.,White A, Ding X, vanderSpek JC, Murphy JR, Ringe D Nature. 1998 Jul 30;394(6692):502-6. PMID:9697776<ref>PMID:9697776</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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<div class="pdbe-citations 1ddn" style="background-color:#fffaf0;"></div>
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==See Also==
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The line below this paragraph, {{ABSTRACT_PUBMED_9697776}}, adds the Publication Abstract to the page
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*[[Diphtheria toxin repressor|Diphtheria toxin repressor]]
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(as it appears on PubMed at http://www.pubmed.gov), where 9697776 is the PubMed ID number.
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== References ==
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<references/>
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{{ABSTRACT_PUBMED_9697776}}
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__TOC__
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</StructureSection>
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==About this Structure==
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1DDN is a 6 chains structure of sequences from [http://en.wikipedia.org/wiki/Corynebacterium_diphtheriae Corynebacterium diphtheriae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1DDN OCA].
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==Reference==
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<ref group="xtra">PMID:9697776</ref><references group="xtra"/>
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[[Category: Corynebacterium diphtheriae]]
[[Category: Corynebacterium diphtheriae]]
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[[Category: Ding, X.]]
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[[Category: Large Structures]]
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[[Category: Murphy, J R.]]
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[[Category: Ding X]]
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[[Category: Ringe, D.]]
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[[Category: Murphy JR]]
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[[Category: Vanderspek, J C.]]
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[[Category: Ringe D]]
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[[Category: White, A.]]
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[[Category: Vanderspek JC]]
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[[Category: Diphtheria tox repressor]]
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[[Category: White A]]
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[[Category: Dna- protein]]
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[[Category: Dna-binding regulatory protein]]
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[[Category: Dna-repressor complex]]
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[[Category: Iron-regulated repressor]]
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[[Category: Transcription regulation]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Feb 16 09:55:54 2009''
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Current revision

DIPHTHERIA TOX REPRESSOR (C102D MUTANT)/TOX DNA OPERATOR COMPLEX

PDB ID 1ddn

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