1jh4

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{{Seed}}
 
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[[Image:1jh4.png|left|200px]]
 
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==Solution structure of the C-terminal PABC domain of human poly(A)-binding protein in complex with the peptide from Paip1==
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The line below this paragraph, containing "STRUCTURE_1jh4", creates the "Structure Box" on the page.
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<StructureSection load='1jh4' size='340' side='right'caption='[[1jh4]]' scene=''>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>[[1jh4]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1JH4 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1JH4 FirstGlance]. <br>
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or leave the SCENE parameter empty for the default display.
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1jh4 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1jh4 OCA], [https://pdbe.org/1jh4 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1jh4 RCSB], [https://www.ebi.ac.uk/pdbsum/1jh4 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1jh4 ProSAT]</span></td></tr>
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{{STRUCTURE_1jh4| PDB=1jh4 | SCENE= }}
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/PABP1_HUMAN PABP1_HUMAN] Binds the poly(A) tail of mRNA. May be involved in cytoplasmic regulatory processes of mRNA metabolism such as pre-mRNA splicing. Its function in translational initiation regulation can either be enhanced by PAIP1 or repressed by PAIP2. Can probably bind to cytoplasmic RNA sequences other than poly(A) in vivo. Involved in translationally coupled mRNA turnover. Implicated with other RNA-binding proteins in the cytoplasmic deadenylation/translational and decay interplay of the FOS mRNA mediated by the major coding-region determinant of instability (mCRD) domain. Involved in regulation of nonsense-mediated decay (NMD) of mRNAs containing premature stop codons; for the recognition of premature termination codons (PTC) and initiation of NMD a competitive interaction between UPF1 and PABPC1 with the ribosome-bound release factors is proposed.<ref>PMID:11051545</ref> <ref>PMID:18447585</ref>
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/jh/1jh4_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1jh4 ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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The C-terminal domain of poly(A)-binding protein (PABC) is a peptide-binding domain found in poly(A)-binding proteins (PABPs) and a HECT (homologous to E6-AP C-terminus) family E3 ubiquitin ligase. In protein synthesis, the PABC domain of PABP functions to recruit several translation factors possessing the PABP-interacting motif 2 (PAM2) to the mRNA poly(A) tail. We have determined the solution structure of the human PABC domain in complex with two peptides from PABP-interacting protein-1 (Paip1) and Paip2. The structures show a novel mode of peptide recognition, in which the peptide binds as a pair of beta-turns with extensive hydrophobic, electrostatic and aromatic stacking interactions. Mutagenesis of PABC and peptide residues was used to identify key protein-peptide interactions and quantified by isothermal calorimetry, surface plasmon resonance and GST pull-down assays. The results provide insight into the specificity of PABC in mediating PABP-protein interactions.
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===Solution structure of the C-terminal PABC domain of human poly(A)-binding protein in complex with the peptide from Paip1===
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Structural basis of ligand recognition by PABC, a highly specific peptide-binding domain found in poly(A)-binding protein and a HECT ubiquitin ligase.,Kozlov G, De Crescenzo G, Lim NS, Siddiqui N, Fantus D, Kahvejian A, Trempe JF, Elias D, Ekiel I, Sonenberg N, O'Connor-McCourt M, Gehring K EMBO J. 2004 Jan 28;23(2):272-81. Epub 2003 Dec 18. PMID:14685257<ref>PMID:14685257</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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The line below this paragraph, {{ABSTRACT_PUBMED_14685257}}, adds the Publication Abstract to the page
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<div class="pdbe-citations 1jh4" style="background-color:#fffaf0;"></div>
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(as it appears on PubMed at http://www.pubmed.gov), where 14685257 is the PubMed ID number.
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== References ==
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<references/>
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{{ABSTRACT_PUBMED_14685257}}
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__TOC__
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</StructureSection>
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==About this Structure==
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1JH4 is a 2 chains structure of sequences from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1JH4 OCA].
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==Reference==
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<ref group="xtra">PMID:14685257</ref><references group="xtra"/>
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[[Category: Homo sapiens]]
[[Category: Homo sapiens]]
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[[Category: Coillet-Matillon, S.]]
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[[Category: Large Structures]]
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[[Category: Ekiel, I.]]
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[[Category: Coillet-Matillon S]]
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[[Category: Gehring, K.]]
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[[Category: Ekiel I]]
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[[Category: Kozlov, G.]]
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[[Category: Gehring K]]
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[[Category: Siddiqui, N.]]
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[[Category: Kozlov G]]
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[[Category: All-helical domain]]
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[[Category: Siddiqui N]]
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[[Category: Protein-peptide complex]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Feb 16 10:24:54 2009''
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Current revision

Solution structure of the C-terminal PABC domain of human poly(A)-binding protein in complex with the peptide from Paip1

PDB ID 1jh4

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OCA

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