1hcu

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{{Seed}}
 
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[[Image:1hcu.png|left|200px]]
 
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==alpha-1,2-mannosidase from Trichoderma reesei==
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The line below this paragraph, containing "STRUCTURE_1hcu", creates the "Structure Box" on the page.
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<StructureSection load='1hcu' size='340' side='right'caption='[[1hcu]], [[Resolution|resolution]] 2.37&Aring;' scene=''>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>[[1hcu]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Trichoderma_reesei Trichoderma reesei]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1HCU OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1HCU FirstGlance]. <br>
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or leave the SCENE parameter empty for the default display.
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.37&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene></td></tr>
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{{STRUCTURE_1hcu| PDB=1hcu | SCENE= }}
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1hcu FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1hcu OCA], [https://pdbe.org/1hcu PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1hcu RCSB], [https://www.ebi.ac.uk/pdbsum/1hcu PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1hcu ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/Q9P8T8_HYPJE Q9P8T8_HYPJE]
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/hc/1hcu_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview03.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1hcu ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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The process of N-glycosylation of eukaryotic proteins involves a range of host enzymes that delete or add saccharide monomers. While endoplasmic reticulum (E.R.) mannosidases cleave only one mannose to produce the Man8B isomer, an alpha-1,2-mannosidase from Trichoderma reesei can sequentially cleave all four 1,2-linked mannose sugars from a Man(9)GlcNAc(2) oligosaccharide, a feature reminiscent of the activity of Golgi mannosidases. We now report the structure of the T. reesei enzyme at 2.37 A resolution. The enzyme folds as an (alpha alpha)(7) barrel. The substrate-binding site of the T. reesei mannosidase differs appreciably from the Saccharomyces cerevisiae enzyme. In the former, shorter loops at the surface allow substrate protein to come closer to the catalytic site. There is more internal space available, so that different oligosaccharide conformations are sterically allowed in the T. reesei alpha-1,2-mannosidase.
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===ALPHA-1,2-MANNOSIDASE FROM TRICHODERMA REESEI===
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Trichoderma reesei alpha-1,2-mannosidase: structural basis for the cleavage of four consecutive mannose residues.,Van Petegem F, Contreras H, Contreras R, Van Beeumen J J Mol Biol. 2001 Sep 7;312(1):157-65. PMID:11545593<ref>PMID:11545593</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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<div class="pdbe-citations 1hcu" style="background-color:#fffaf0;"></div>
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==See Also==
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The line below this paragraph, {{ABSTRACT_PUBMED_11545593}}, adds the Publication Abstract to the page
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*[[Mannosidase 3D structures|Mannosidase 3D structures]]
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(as it appears on PubMed at http://www.pubmed.gov), where 11545593 is the PubMed ID number.
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== References ==
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<references/>
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{{ABSTRACT_PUBMED_11545593}}
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__TOC__
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</StructureSection>
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==About this Structure==
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[[Category: Large Structures]]
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1HCU is a 4 chains structure of sequences from [http://en.wikipedia.org/wiki/Hypocrea_jecorina Hypocrea jecorina]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1HCU OCA].
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[[Category: Trichoderma reesei]]
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[[Category: Contreras H]]
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==Reference==
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[[Category: Contreras R]]
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<ref group="xtra">PMID:11545593</ref><references group="xtra"/>
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[[Category: Van Beeumen J]]
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[[Category: Hypocrea jecorina]]
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[[Category: Van Petegem F]]
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[[Category: Mannosyl-oligosaccharide 1,2-alpha-mannosidase]]
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[[Category: Beeumen, J Van.]]
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[[Category: Contreras, H.]]
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[[Category: Contreras, R.]]
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[[Category: Petegem, F Van.]]
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[[Category: Glycosyl hydrolase]]
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[[Category: Glycosylation]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Feb 16 10:26:49 2009''
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Current revision

alpha-1,2-mannosidase from Trichoderma reesei

PDB ID 1hcu

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