1x8d

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(New page: 200px<br /><applet load="1x8d" size="450" color="white" frame="true" align="right" spinBox="true" caption="1x8d, resolution 1.8&Aring;" /> '''Crystal structure of ...)
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[[Image:1x8d.gif|left|200px]]<br /><applet load="1x8d" size="450" color="white" frame="true" align="right" spinBox="true"
 
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caption="1x8d, resolution 1.8&Aring;" />
 
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'''Crystal structure of E. coli YiiL protein containing L-rhamnose'''<br />
 
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==Overview==
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==Crystal structure of E. coli YiiL protein containing L-rhamnose==
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The crystal structure of Escherichia coli rhamnose mutarotase (YiiL) is, completely different from the previously reported structures of the, Lactococcus lactis galactose mutarotase and the Bacillus subtilis RbsD, (pyranase). YiiL exists as a locally asymmetric dimer, which is stabilized, by an intermolecular beta-sheet, various hydrophobic interactions, and a, cation-pi interaction with a salt-bridge. The protein folds of YiiL are, similar to those of a Streptomyces coelicolor mono-oxygenase and a, hypothetical Arabidopsis thaliana protein At3g17210. By assaying the, enzymatic activity of six active-site mutants and by comparing the crystal, structure-derived active site conformations of YiiL, RbsD, and a galactose, mutarotase, we were able to define the amino acid residues required for, catalysis and suggest a possible catalytic mechanism for YiiL. Although, the active-site amino acid residues of YiiL (His, Tyr, and Trp) differ, greatly from those of galactose mutarotase (His, Glu, and Asp), their, geometries, which determine the structures of the preferred monosaccharide, substrates, are conserved. In addition, the in vivo function of YiiL was, assessed by constructing a mutant E.coli strain that carries a yiiL, deletion. The presence of the yiiL gene is critical for efficient cell, growth only when concentrations of l-rhamnose are limited.
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<StructureSection load='1x8d' size='340' side='right'caption='[[1x8d]], [[Resolution|resolution]] 1.80&Aring;' scene=''>
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== Structural highlights ==
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==About this Structure==
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<table><tr><td colspan='2'>[[1x8d]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1X8D OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1X8D FirstGlance]. <br>
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1X8D is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli] with RNS as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1X8D OCA].
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.8&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=RNS:L-RHAMNOSE'>RNS</scene></td></tr>
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==Reference==
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1x8d FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1x8d OCA], [https://pdbe.org/1x8d PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1x8d RCSB], [https://www.ebi.ac.uk/pdbsum/1x8d PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1x8d ProSAT]</span></td></tr>
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Structural insights into the monosaccharide specificity of Escherichia coli rhamnose mutarotase., Ryu KS, Kim JI, Cho SJ, Park D, Park C, Cheong HK, Lee JO, Choi BS, J Mol Biol. 2005 May 27;349(1):153-62. Epub 2005 Apr 7. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=15876375 15876375]
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/RHAM_ECOLI RHAM_ECOLI] Involved in the anomeric conversion of L-rhamnose.<ref>PMID:15060078</ref>
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/x8/1x8d_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1x8d ConSurf].
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<div style="clear:both"></div>
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== References ==
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<references/>
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__TOC__
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</StructureSection>
[[Category: Escherichia coli]]
[[Category: Escherichia coli]]
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[[Category: Single protein]]
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[[Category: Large Structures]]
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[[Category: Cho, S.J.]]
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[[Category: Cho SJ]]
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[[Category: Choi, B.S.]]
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[[Category: Choi BS]]
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[[Category: Kim, J.I.]]
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[[Category: Kim JI]]
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[[Category: Lee, J.O.]]
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[[Category: Lee JO]]
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[[Category: Park, C.]]
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[[Category: Park C]]
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[[Category: Park, D.]]
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[[Category: Park D]]
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[[Category: Ryu, K.S.]]
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[[Category: Ryu KS]]
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[[Category: RNS]]
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[[Category: l-rhamnose]]
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[[Category: mutarotase]]
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''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Wed Nov 21 05:53:30 2007''
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Current revision

Crystal structure of E. coli YiiL protein containing L-rhamnose

PDB ID 1x8d

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