1xao

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(New page: 200px<br /><applet load="1xao" size="450" color="white" frame="true" align="right" spinBox="true" caption="1xao, resolution 2.07&Aring;" /> '''Hsp40-Ydj1 dimerizat...)
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[[Image:1xao.gif|left|200px]]<br /><applet load="1xao" size="450" color="white" frame="true" align="right" spinBox="true"
 
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caption="1xao, resolution 2.07&Aring;" />
 
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'''Hsp40-Ydj1 dimerization domain'''<br />
 
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==Overview==
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==Hsp40-Ydj1 dimerization domain==
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The molecular chaperone Hsp40 functions as a dimer. The dimer formation is, critical for Hsp40 molecular chaperone activity to facilitate Hsp70 to, refold non-native polypeptides. We have determined the crystal structure, of the C-terminal fragment of yeast Hsp40 Ydj1 that is responsible for, Ydj1 dimerization by MAD method. The C-terminal fragment of Ydj1 comprises, of the domain III of Ydj1 and the Ydj1 C-terminal dimerization motif. The, crystal structure indicates that the dimerization motif of type I Hsp40, Ydj1 differs significantly from that of yeast type II Hsp40. The C, terminus of type I Hsp40 Ydj1 from one monomer forms beta-strands with the, domain III from the other monomer in the homo-dimer. The L372 from Ydj1 C, terminus inserts its side-chain into a hydrophobic pocket on domain III., The modeled full-length Ydj1 dimer structure reveals that a large cleft is, formed between the two monomers. The domain IIs of Ydj1 monomers that, contain the zinc-finger motifs points directly against each other.
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<StructureSection load='1xao' size='340' side='right'caption='[[1xao]], [[Resolution|resolution]] 2.07&Aring;' scene=''>
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== Structural highlights ==
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==About this Structure==
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<table><tr><td colspan='2'>[[1xao]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1XAO OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1XAO FirstGlance]. <br>
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1XAO is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1XAO OCA].
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.07&#8491;</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1xao FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1xao OCA], [https://pdbe.org/1xao PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1xao RCSB], [https://www.ebi.ac.uk/pdbsum/1xao PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1xao ProSAT]</span></td></tr>
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==Reference==
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</table>
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The crystal structure of the C-terminal fragment of yeast Hsp40 Ydj1 reveals novel dimerization motif for Hsp40., Wu Y, Li J, Jin Z, Fu Z, Sha B, J Mol Biol. 2005 Mar 4;346(4):1005-11. Epub 2005 Jan 16. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=15701512 15701512]
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== Function ==
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[https://www.uniprot.org/uniprot/MAS5_YEAST MAS5_YEAST] Probably involved in mitochondrial protein import. Is also required for efficient translocation of pre-pro-alpha-factor. Involved in heme regulation of HAP1, as a component of the high-molecular-weight (HMC) complex.<ref>PMID:11689685</ref>
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/xa/1xao_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1xao ConSurf].
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<div style="clear:both"></div>
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== References ==
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<references/>
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__TOC__
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</StructureSection>
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[[Category: Large Structures]]
[[Category: Saccharomyces cerevisiae]]
[[Category: Saccharomyces cerevisiae]]
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[[Category: Single protein]]
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[[Category: Sha B]]
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[[Category: Sha, B.]]
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[[Category: Wu Y]]
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[[Category: Wu, Y.]]
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[[Category: beta sheets]]
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''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Wed Nov 21 05:56:56 2007''
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Current revision

Hsp40-Ydj1 dimerization domain

PDB ID 1xao

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