1xas

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(New page: 200px<br /><applet load="1xas" size="450" color="white" frame="true" align="right" spinBox="true" caption="1xas, resolution 2.6&Aring;" /> '''CRYSTAL STRUCTURE, AT...)
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[[Image:1xas.gif|left|200px]]<br /><applet load="1xas" size="450" color="white" frame="true" align="right" spinBox="true"
 
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caption="1xas, resolution 2.6&Aring;" />
 
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'''CRYSTAL STRUCTURE, AT 2.6 ANGSTROMS RESOLUTION, OF THE STREPTOMYCES LIVIDANS XYLANASE A, A MEMBER OF THE F FAMILY OF BETA-1,4-D-GLYCANSES'''<br />
 
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==Overview==
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==CRYSTAL STRUCTURE, AT 2.6 ANGSTROMS RESOLUTION, OF THE STREPTOMYCES LIVIDANS XYLANASE A, A MEMBER OF THE F FAMILY OF BETA-1,4-D-GLYCANSES==
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The crystal structure of the 32-kDa catalytic domain of the Streptomyces, lividans xylanase A was solved by molecular isomorphous replacement, methods and subsequently refined at 2.6-A resolution to a conventional, crystallographic R factor of 0.21. This is the first successful structure, determination of a member of the F family of endo-beta-1,4-D-glycanases., Unlike the recently determined xylanases of the G family (Wakarchuk, W., W., Campbell, R. L., Sung, W. L., Davoodi, J., and Yaguchi, M. (1994), Protein Sci. 3, 467-475), where the catalytic domains have a unique, beta-sheet structure, the 32-kDa domain of the S. lividans xylanase A is, folded into a complete (alpha/beta)8 barrel, the first such fold observed, among beta-1,4-D-glycanases. The active site is located at the carbonyl, end of the beta barrel. The crystal structure supports the earlier, assignment of Glu-128 and Glu-236 as the catalytic amino acids (Moreau, A., Roberge, M., Manin, C., Shareck, F., Kluepfel, D., and Morosoli, R., (1994) Biochem. J., in press).
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<StructureSection load='1xas' size='340' side='right'caption='[[1xas]], [[Resolution|resolution]] 2.60&Aring;' scene=''>
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== Structural highlights ==
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==About this Structure==
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<table><tr><td colspan='2'>[[1xas]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Streptomyces_lividans Streptomyces lividans]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1XAS OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1XAS FirstGlance]. <br>
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1XAS is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Streptomyces_lividans Streptomyces lividans]. Active as [http://en.wikipedia.org/wiki/Endo-1,4-beta-xylanase Endo-1,4-beta-xylanase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.8 3.2.1.8] Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1XAS OCA].
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.6&#8491;</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1xas FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1xas OCA], [https://pdbe.org/1xas PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1xas RCSB], [https://www.ebi.ac.uk/pdbsum/1xas PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1xas ProSAT]</span></td></tr>
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==Reference==
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</table>
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Crystal structure, at 2.6-A resolution, of the Streptomyces lividans xylanase A, a member of the F family of beta-1,4-D-glycanases., Derewenda U, Swenson L, Green R, Wei Y, Morosoli R, Shareck F, Kluepfel D, Derewenda ZS, J Biol Chem. 1994 Aug 19;269(33):20811-4. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=8063693 8063693]
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== Function ==
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[[Category: Endo-1,4-beta-xylanase]]
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[https://www.uniprot.org/uniprot/XYNA_STRLI XYNA_STRLI] Contributes to hydrolyze hemicellulose, the major component of plant cell-walls. XLNA and XLNB seem to act sequentially on the substrate to yield xylobiose and xylose as carbon sources.
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[[Category: Single protein]]
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/xa/1xas_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1xas ConSurf].
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<div style="clear:both"></div>
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__TOC__
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</StructureSection>
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[[Category: Large Structures]]
[[Category: Streptomyces lividans]]
[[Category: Streptomyces lividans]]
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[[Category: Derewenda, U.]]
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[[Category: Derewenda U]]
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[[Category: Derewenda, Z.S.]]
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[[Category: Derewenda ZS]]
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[[Category: xylanase a]]
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''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Wed Nov 21 05:57:00 2007''
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Current revision

CRYSTAL STRUCTURE, AT 2.6 ANGSTROMS RESOLUTION, OF THE STREPTOMYCES LIVIDANS XYLANASE A, A MEMBER OF THE F FAMILY OF BETA-1,4-D-GLYCANSES

PDB ID 1xas

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