2hqa

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{{Seed}}
 
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[[Image:2hqa.png|left|200px]]
 
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==Crystal structure of the catalytic alpha subunit of E. Coli replicative DNA polymerase III==
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The line below this paragraph, containing "STRUCTURE_2hqa", creates the "Structure Box" on the page.
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<StructureSection load='2hqa' size='340' side='right'caption='[[2hqa]], [[Resolution|resolution]] 2.60&Aring;' scene=''>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>[[2hqa]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/"bacillus_coli"_migula_1895 "bacillus coli" migula 1895]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2HQA OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2HQA FirstGlance]. <br>
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or leave the SCENE parameter empty for the default display.
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</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene></td></tr>
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<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr>
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{{STRUCTURE_2hqa| PDB=2hqa | SCENE= }}
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<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[2hnh|2hnh]]</div></td></tr>
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<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">dnaE ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=562 "Bacillus coli" Migula 1895])</td></tr>
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<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[https://en.wikipedia.org/wiki/DNA-directed_DNA_polymerase DNA-directed DNA polymerase], with EC number [https://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.7.7 2.7.7.7] </span></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2hqa FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2hqa OCA], [https://pdbe.org/2hqa PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2hqa RCSB], [https://www.ebi.ac.uk/pdbsum/2hqa PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2hqa ProSAT]</span></td></tr>
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</table>
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== Function ==
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[[https://www.uniprot.org/uniprot/DPO3A_ECOLI DPO3A_ECOLI]] DNA polymerase III is a complex, multichain enzyme responsible for most of the replicative synthesis in bacteria. This DNA polymerase also exhibits 3' to 5' exonuclease activity. The alpha chain is the DNA polymerase.
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/hq/2hqa_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2hqa ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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Bacterial replicative DNA polymerases such as Polymerase III (Pol III) share no sequence similarity with other polymerases. The crystal structure, determined at 2.3 A resolution, of a large fragment of Pol III (residues 1-917), reveals a unique chain fold with localized similarity in the catalytic domain to DNA polymerase beta and related nucleotidyltransferases. The structure of Pol III is strikingly different from those of members of the canonical DNA polymerase families, which include eukaryotic replicative polymerases, suggesting that the DNA replication machinery in bacteria arose independently. A structural element near the active site in Pol III that is not present in nucleotidyltransferases but which resembles an element at the active sites of some canonical DNA polymerases suggests that, at a more distant level, all DNA polymerases may share a common ancestor. The structure also suggests a model for interaction of Pol III with the sliding clamp and DNA.
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===Crystal structure of the catalytic alpha subunit of E. Coli replicative DNA polymerase III===
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Crystal structure of the catalytic alpha subunit of E. coli replicative DNA polymerase III.,Lamers MH, Georgescu RE, Lee SG, O'Donnell M, Kuriyan J Cell. 2006 Sep 8;126(5):881-92. PMID:16959568<ref>PMID:16959568</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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The line below this paragraph, {{ABSTRACT_PUBMED_16959568}}, adds the Publication Abstract to the page
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<div class="pdbe-citations 2hqa" style="background-color:#fffaf0;"></div>
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(as it appears on PubMed at http://www.pubmed.gov), where 16959568 is the PubMed ID number.
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== References ==
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<references/>
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{{ABSTRACT_PUBMED_16959568}}
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__TOC__
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</StructureSection>
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==About this Structure==
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[[Category: Bacillus coli migula 1895]]
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2HQA is a 1 chain structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2HQA OCA].
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==Reference==
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<ref group="xtra">PMID:16959568</ref><references group="xtra"/>
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[[Category: DNA-directed DNA polymerase]]
[[Category: DNA-directed DNA polymerase]]
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[[Category: Escherichia coli]]
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[[Category: Large Structures]]
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[[Category: Donnell, M O.]]
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[[Category: Donnell, M O]]
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[[Category: Georgescu, R E.]]
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[[Category: Georgescu, R E]]
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[[Category: Kuriyan, J.]]
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[[Category: Kuriyan, J]]
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[[Category: Lamers, M H.]]
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[[Category: Lamers, M H]]
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[[Category: Lee, S G.]]
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[[Category: Lee, S G]]
[[Category: Dna polymerase iii]]
[[Category: Dna polymerase iii]]
[[Category: Dna replication]]
[[Category: Dna replication]]
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[[Category: Php]]
[[Category: Php]]
[[Category: Pol beta]]
[[Category: Pol beta]]
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[[Category: Transferase]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Feb 16 11:26:46 2009''
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Current revision

Crystal structure of the catalytic alpha subunit of E. Coli replicative DNA polymerase III

PDB ID 2hqa

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