1zj8

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{{Seed}}
 
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[[Image:1zj8.png|left|200px]]
 
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==Structure of Mycobacterium tuberculosis NirA protein==
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The line below this paragraph, containing "STRUCTURE_1zj8", creates the "Structure Box" on the page.
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<StructureSection load='1zj8' size='340' side='right'caption='[[1zj8]], [[Resolution|resolution]] 2.80&Aring;' scene=''>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>[[1zj8]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Mycobacterium_tuberculosis_H37Rv Mycobacterium tuberculosis H37Rv]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1ZJ8 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1ZJ8 FirstGlance]. <br>
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or leave the SCENE parameter empty for the default display.
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.8&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=SRM:SIROHEME'>SRM</scene></td></tr>
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{{STRUCTURE_1zj8| PDB=1zj8 | SCENE= }}
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1zj8 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1zj8 OCA], [https://pdbe.org/1zj8 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1zj8 RCSB], [https://www.ebi.ac.uk/pdbsum/1zj8 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1zj8 ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/SIR_MYCTU SIR_MYCTU]
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/zj/1zj8_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview03.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1zj8 ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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The nirA gene of Mycobacterium tuberculosis is up-regulated in the persistent state of the bacteria, suggesting that it is a potential target for the development of antituberculosis agents particularly active against the pathogen in its dormant phase. This gene encodes a ferredoxin-dependent sulfite reductase, and the structure of the enzyme has been determined using x-ray crystallography. The enzyme is a monomer comprising 555 amino acids and contains a [Fe4-S4] cluster and a siroheme cofactor. The molecule is built up of three domains with an alpha/beta fold. The first domain consists of two ferredoxin-like subdomains, related by a pseudo-2-fold symmetry axis passing through the whole molecule. The other two domains, which provide much of the binding interactions with the cofactors, have a common fold that is unique to the sulfite/nitrite reductase family. The domains form a trilobal structure, with the cofactors and the active site located at the interface of all three domains in the center of the molecule. NirA contains an unusual covalent bond between the side chains of Tyr69 and Cys161 in the active site, in close proximity to the siroheme cofactor. Removal of this covalent bond by site-directed mutagenesis impairs catalytic activity, suggesting that it is important for the enzymatic reaction. These residues are part of a sequence fingerprint, able to distinguish between ferredoxin-dependent sulfite and nitrite reductases. Comparison of NirA with the structure of the truncated NADPH-dependent sulfite reductase from Escherichia coli suggests a binding site for the external electron donor ferredoxin close to the [Fe4-S4] cluster.
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===Structure of Mycobacterium tuberculosis NirA protein===
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Siroheme- and [Fe4-S4]-dependent NirA from Mycobacterium tuberculosis is a sulfite reductase with a covalent Cys-Tyr bond in the active site.,Schnell R, Sandalova T, Hellman U, Lindqvist Y, Schneider G J Biol Chem. 2005 Jul 22;280(29):27319-28. Epub 2005 May 24. PMID:15917234<ref>PMID:15917234</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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The line below this paragraph, {{ABSTRACT_PUBMED_15917234}}, adds the Publication Abstract to the page
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<div class="pdbe-citations 1zj8" style="background-color:#fffaf0;"></div>
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(as it appears on PubMed at http://www.pubmed.gov), where 15917234 is the PubMed ID number.
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== References ==
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<references/>
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{{ABSTRACT_PUBMED_15917234}}
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__TOC__
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</StructureSection>
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==About this Structure==
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[[Category: Large Structures]]
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1ZJ8 is a 2 chains structure of sequences from [http://en.wikipedia.org/wiki/Mycobacterium_tuberculosis Mycobacterium tuberculosis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1ZJ8 OCA].
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[[Category: Mycobacterium tuberculosis H37Rv]]
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[[Category: Hellman U]]
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==Reference==
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[[Category: Lindqvist Y]]
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<ref group="xtra">PMID:15917234</ref><references group="xtra"/>
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[[Category: Sandalova T]]
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[[Category: Ferredoxin--nitrite reductase]]
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[[Category: Schneider G]]
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[[Category: Mycobacterium tuberculosis]]
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[[Category: Schnell R]]
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[[Category: Hellman, U.]]
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[[Category: Lindqvist, Y.]]
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[[Category: SPINE, Structural Proteomics in Europe.]]
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[[Category: Sandalova, T.]]
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[[Category: Schneider, G.]]
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[[Category: Schnell, R.]]
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[[Category: Cys-tyr covalent bond]]
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[[Category: Fe4-s4]]
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[[Category: Nira]]
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[[Category: Nitrite]]
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[[Category: Reductase]]
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[[Category: Siroheme]]
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[[Category: Spine]]
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[[Category: Structural genomic]]
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[[Category: Structural proteomics in europe]]
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[[Category: Sulfite]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Feb 16 11:40:41 2009''
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Current revision

Structure of Mycobacterium tuberculosis NirA protein

PDB ID 1zj8

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