2pic

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{{Seed}}
 
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[[Image:2pic.png|left|200px]]
 
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==E. coli lytic transglycosylase MltA-D308A in apo-2 form==
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The line below this paragraph, containing "STRUCTURE_2pic", creates the "Structure Box" on the page.
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<StructureSection load='2pic' size='340' side='right'caption='[[2pic]], [[Resolution|resolution]] 2.25&Aring;' scene=''>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>[[2pic]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli_K-12 Escherichia coli K-12]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2PIC OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2PIC FirstGlance]. <br>
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or leave the SCENE parameter empty for the default display.
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.25&#8491;</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2pic FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2pic OCA], [https://pdbe.org/2pic PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2pic RCSB], [https://www.ebi.ac.uk/pdbsum/2pic PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2pic ProSAT]</span></td></tr>
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{{STRUCTURE_2pic| PDB=2pic | SCENE= }}
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/MLTA_ECOLI MLTA_ECOLI] Murein-degrading enzyme. May play a role in recycling of muropeptides during cell elongation and/or cell division. Degrades murein glycan strands and insoluble, high-molecular weight murein sacculi.
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/pi/2pic_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2pic ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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Crystal structures of an inactive mutant (D308A) of the lytic transglycosylase MltA from Escherichia coli have been determined in two different apo-forms, as well as in complex with the substrate analogue chitohexaose. The chitohexaose binds with all six saccharide residues in the active site groove, with an intact glycosidic bond at the bond cleavage center. Its binding induces a large reorientation of the two structural domains in MltA, narrowing the active site groove and allowing tight interactions of the oligosaccharide with residues from both domains. The structures identify residues in MltA with key roles in the binding and recognition of peptidoglycan and confirm that Asp-308 is the single catalytic residue, acting as a general acid/base. Moreover, the structures suggest that catalysis involves a high energy conformation of the scissile glycosidic linkage and that the putative oxocarbenium ion intermediate is stabilized by the dipole moment of a nearby alpha-helix.
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===E. coli lytic transglycosylase MltA-D308A in apo-2 form===
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Structure of Escherichia coli Lytic transglycosylase MltA with bound chitohexaose: implications for peptidoglycan binding and cleavage.,van Straaten KE, Barends TR, Dijkstra BW, Thunnissen AM J Biol Chem. 2007 Jul 20;282(29):21197-205. Epub 2007 May 14. PMID:17502382<ref>PMID:17502382</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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The line below this paragraph, {{ABSTRACT_PUBMED_17502382}}, adds the Publication Abstract to the page
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<div class="pdbe-citations 2pic" style="background-color:#fffaf0;"></div>
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(as it appears on PubMed at http://www.pubmed.gov), where 17502382 is the PubMed ID number.
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== References ==
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<references/>
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{{ABSTRACT_PUBMED_17502382}}
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__TOC__
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</StructureSection>
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==About this Structure==
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[[Category: Escherichia coli K-12]]
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2PIC is a 1 chain structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2PIC OCA].
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[[Category: Large Structures]]
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[[Category: Dijkstra BW]]
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==Reference==
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[[Category: Thunnissen AMWH]]
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<ref group="xtra">PMID:17502382</ref><references group="xtra"/>
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[[Category: Van Straaten KE]]
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[[Category: Escherichia coli]]
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[[Category: Dijkstra, B W.]]
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[[Category: Straaten, K E.van.]]
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[[Category: Thunnissen, A M.W H.]]
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[[Category: Active site mutant]]
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[[Category: Double-psi beta-barrel]]
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[[Category: Lytic transglycosylase]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Feb 16 11:59:19 2009''
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Current revision

E. coli lytic transglycosylase MltA-D308A in apo-2 form

PDB ID 2pic

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