2h1k

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{{Seed}}
 
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[[Image:2h1k.png|left|200px]]
 
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==Crystal structure of the Pdx1 homeodomain in complex with DNA==
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The line below this paragraph, containing "STRUCTURE_2h1k", creates the "Structure Box" on the page.
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<StructureSection load='2h1k' size='340' side='right'caption='[[2h1k]], [[Resolution|resolution]] 2.42&Aring;' scene=''>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>[[2h1k]] is a 6 chain structure with sequence from [https://en.wikipedia.org/wiki/Mesocricetus_auratus Mesocricetus auratus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2H1K OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2H1K FirstGlance]. <br>
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or leave the SCENE parameter empty for the default display.
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.42&#8491;</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2h1k FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2h1k OCA], [https://pdbe.org/2h1k PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2h1k RCSB], [https://www.ebi.ac.uk/pdbsum/2h1k PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2h1k ProSAT]</span></td></tr>
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{{STRUCTURE_2h1k| PDB=2h1k | SCENE= }}
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/PDX1_MESAU PDX1_MESAU] Activates insulin and somatostatin gene transcription. Key regulator of islet peptide hormone expression but also responsible for the development of the pancreas, most probably by determining maturation and differentiation of common pancreatic precursor cells in the developing gut. As part of a PDX1:PBX1b:MEIS2b complex in pancreatic acinar cells is involved in the transcriptional activation of the ELA1 enhancer; the complex binds to the enhancer B element and cooperates with the transcription factor 1 complex (PTF1) bound to the enhancer A element. Binds the DNA sequence 5'-CC[CT]TAATGGG-3' (By similarity).
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/h1/2h1k_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2h1k ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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Pancreatic and duodenal homeobox 1 (Pdx1) is a homeodomain transcription factor belonging to the ParaHox family. Pdx1 plays an essential role in pancreatic endocrine and exocrine cell development and maintenance of adult islet beta-cell function. Mutations in the human pdx1 gene are linked to an early onset form of non-insulin-dependent diabetes mellitus, MODY-4. We demonstrate that the homeodomain reproduces the binding specificity of the full-length protein. We report the 2.4 A resolution crystal structure of the homeodomain bound to a target DNA. The two Pdx1/DNA complexes in the asymmetric unit display conformational differences: in the DNA curvature, the orientation of the homeodomain in the major groove, and the order of the N-terminal arm. Comparing the two complexes indicates invariant protein-DNA contacts, and variant contacts that are unique to each binding orientation. An induced fit model is proposed that depends on the DNA conformation and provides a mechanism for nonlocal contributions to binding specificity.
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===Crystal structure of the Pdx1 homeodomain in complex with DNA===
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Structural basis for induced fit mechanisms in DNA recognition by the Pdx1 homeodomain.,Longo A, Guanga GP, Rose RB Biochemistry. 2007 Mar 20;46(11):2948-57. Epub 2007 Feb 23. PMID:17315980<ref>PMID:17315980</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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The line below this paragraph, {{ABSTRACT_PUBMED_17315980}}, adds the Publication Abstract to the page
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<div class="pdbe-citations 2h1k" style="background-color:#fffaf0;"></div>
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(as it appears on PubMed at http://www.pubmed.gov), where 17315980 is the PubMed ID number.
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== References ==
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<references/>
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{{ABSTRACT_PUBMED_17315980}}
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__TOC__
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</StructureSection>
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==About this Structure==
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[[Category: Large Structures]]
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2H1K is a 6 chains structure of sequences from [http://en.wikipedia.org/wiki/Mesocricetus_auratus Mesocricetus auratus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2H1K OCA].
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==Reference==
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<ref group="xtra">PMID:17315980</ref><references group="xtra"/>
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[[Category: Mesocricetus auratus]]
[[Category: Mesocricetus auratus]]
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[[Category: Guanga, G P.]]
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[[Category: Guanga GP]]
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[[Category: Longo, A.]]
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[[Category: Longo A]]
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[[Category: Rose, R B.]]
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[[Category: Rose RB]]
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[[Category: Protein-dna complex]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Feb 16 12:10:01 2009''
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Current revision

Crystal structure of the Pdx1 homeodomain in complex with DNA

PDB ID 2h1k

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