1o7j

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{{Seed}}
 
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[[Image:1o7j.png|left|200px]]
 
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==Atomic resolution structure of Erwinia chrysanthemi L-asparaginase==
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The line below this paragraph, containing "STRUCTURE_1o7j", creates the "Structure Box" on the page.
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<StructureSection load='1o7j' size='340' side='right'caption='[[1o7j]], [[Resolution|resolution]] 1.00&Aring;' scene=''>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>[[1o7j]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Dickeya_chrysanthemi Dickeya chrysanthemi]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1O7J OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1O7J FirstGlance]. <br>
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or leave the SCENE parameter empty for the default display.
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
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{{STRUCTURE_1o7j| PDB=1o7j | SCENE= }}
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1o7j FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1o7j OCA], [https://pdbe.org/1o7j PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1o7j RCSB], [https://www.ebi.ac.uk/pdbsum/1o7j PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1o7j ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/ASPG_DICCH ASPG_DICCH]
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/o7/1o7j_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1o7j ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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An X-ray structure of L-asparaginase from Erwinia chrysanthemi (ErA) has been refined at 1 A resolution to an R factor of below 0.1, using data collected on a synchrotron source. With four molecules of the enzyme consisting of 327 amino acids each, this crystal contains one of the largest asymmetric units of a protein refined to date at atomic resolution. Previously, structures of ErA and of related enzymes from other bacterial sources have been refined at resolutions not exceeding 1.7 A; thus, the present structure represents a very significant improvement in the quality of the available models of these proteins and should provide a good basis for future studies of the conformational variability of proteins, identification of subtle conformational features and corroboration of the stereochemical libraries, amongst other things. L-Asparaginases, which are enzymes that catalyze the hydrolysis of L-asparagine to aspartic acid, have been used for over 30 y as therapeutic agents in the treatment of acute childhood lymphoblastic leukemia, although the details of the enzymatic reaction and substrate specificity have not yet been completely elucidated. This atomic resolution structure is a step in that direction.
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===ATOMIC RESOLUTION STRUCTURE OF ERWINIA CHRYSANTHEMI L-ASPARAGINASE===
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Atomic resolution structure of Erwinia chrysanthemi L-asparaginase.,Lubkowski J, Dauter M, Aghaiypour K, Wlodawer A, Dauter Z Acta Crystallogr D Biol Crystallogr. 2003 Jan;59(Pt 1):84-92. Epub 2002, Dec 19. PMID:12499544<ref>PMID:12499544</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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<div class="pdbe-citations 1o7j" style="background-color:#fffaf0;"></div>
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==See Also==
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The line below this paragraph, {{ABSTRACT_PUBMED_12499544}}, adds the Publication Abstract to the page
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*[[Asparaginase 3D structures|Asparaginase 3D structures]]
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(as it appears on PubMed at http://www.pubmed.gov), where 12499544 is the PubMed ID number.
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== References ==
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<references/>
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{{ABSTRACT_PUBMED_12499544}}
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__TOC__
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</StructureSection>
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==About this Structure==
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[[Category: Dickeya chrysanthemi]]
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1O7J is a 4 chains structure of sequences from [http://en.wikipedia.org/wiki/Erwinia_chrysanthemi Erwinia chrysanthemi]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1O7J OCA].
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[[Category: Large Structures]]
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[[Category: Aghaiypour K]]
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==Reference==
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[[Category: Dauter M]]
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<ref group="xtra">PMID:12499544</ref><references group="xtra"/>
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[[Category: Dauter Z]]
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[[Category: Asparaginase]]
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[[Category: Lubkowski J]]
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[[Category: Erwinia chrysanthemi]]
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[[Category: Wlodawer A]]
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[[Category: Aghaiypour, K.]]
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[[Category: Dauter, M.]]
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[[Category: Dauter, Z.]]
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[[Category: Lubkowski, J.]]
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[[Category: Wlodawer, A.]]
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[[Category: Atomic resolution,hydrolase]]
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[[Category: L-asparaginase]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Feb 16 12:16:31 2009''
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Current revision

Atomic resolution structure of Erwinia chrysanthemi L-asparaginase

PDB ID 1o7j

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