2nw0

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{{Seed}}
 
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[[Image:2nw0.png|left|200px]]
 
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==Crystal structure of a lysin==
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The line below this paragraph, containing "STRUCTURE_2nw0", creates the "Structure Box" on the page.
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<StructureSection load='2nw0' size='340' side='right'caption='[[2nw0]], [[Resolution|resolution]] 1.60&Aring;' scene=''>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>[[2nw0]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Bacteriophage_sp. Bacteriophage sp.]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2NW0 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2NW0 FirstGlance]. <br>
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or leave the SCENE parameter empty for the default display.
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.6&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ACT:ACETATE+ION'>ACT</scene></td></tr>
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{{STRUCTURE_2nw0| PDB=2nw0 | SCENE= }}
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2nw0 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2nw0 OCA], [https://pdbe.org/2nw0 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2nw0 RCSB], [https://www.ebi.ac.uk/pdbsum/2nw0 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2nw0 ProSAT]</span></td></tr>
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</table>
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/nw/2nw0_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2nw0 ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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Lysins are peptidoglycan hydrolases that are produced by bacteriophage and act to lyse the bacterial host cell wall during progeny phage release. Here, we describe the structure and function of a novel bacteriophage-derived lysin, PlyB, which displays potent lytic activity against the Bacillus anthracis-like strain ATCC 4342. This molecule comprises an N-terminal catalytic domain (PlyB(cat)) and a C-terminal bacterial SH3-like domain, SH3b. It is shown that both domains are required for effective catalytic activity against ATCC 4342. Further, PlyB has specific activity comparable to the phage lysin PlyG, an amidase being developed as a therapeutic against anthrax. In contrast to PlyG, however, the 1.6 A X-ray crystal structure of PlyB(cat) reveals that the catalytic domain adopts the glycosyl hydrolase (GH)-25, rather than phage T7 lysozyme-like fold. PlyB therefore represents a new class of anthrax lysin and a new defensive tool in the armament against anthrax-mediated bioterrorism.
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===Crystal structure of a lysin===
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The 1.6 A crystal structure of the catalytic domain of PlyB, a bacteriophage lysin active against Bacillus anthracis.,Porter CJ, Schuch R, Pelzek AJ, Buckle AM, McGowan S, Wilce MC, Rossjohn J, Russell R, Nelson D, Fischetti VA, Whisstock JC J Mol Biol. 2007 Feb 16;366(2):540-50. Epub 2006 Nov 18. PMID:17182056<ref>PMID:17182056</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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<div class="pdbe-citations 2nw0" style="background-color:#fffaf0;"></div>
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==See Also==
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The line below this paragraph, {{ABSTRACT_PUBMED_17182056}}, adds the Publication Abstract to the page
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*[[Lysin 3D structures|Lysin 3D structures]]
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(as it appears on PubMed at http://www.pubmed.gov), where 17182056 is the PubMed ID number.
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== References ==
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<references/>
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{{ABSTRACT_PUBMED_17182056}}
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__TOC__
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</StructureSection>
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==About this Structure==
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[[Category: Bacteriophage sp]]
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2NW0 is a 2 chains structure of sequences from [http://en.wikipedia.org/wiki/Unidentified_phage Unidentified phage]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2NW0 OCA].
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[[Category: Large Structures]]
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[[Category: Buckle AM]]
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==Reference==
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[[Category: Porter CJ]]
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<ref group="xtra">PMID:17182056</ref><references group="xtra"/>
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[[Category: Whisstock JC]]
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[[Category: Lysozyme]]
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[[Category: Unidentified phage]]
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[[Category: Buckle, A M.]]
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[[Category: Porter, C J.]]
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[[Category: Whisstock, J C.]]
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[[Category: Beta barrel]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Feb 16 12:20:48 2009''
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Current revision

Crystal structure of a lysin

PDB ID 2nw0

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