1xkw

From Proteopedia

(Difference between revisions)
Jump to: navigation, search
(New page: 200px<br /><applet load="1xkw" size="450" color="white" frame="true" align="right" spinBox="true" caption="1xkw, resolution 2.0&Aring;" /> '''Pyochelin outer membr...)
Current revision (06:47, 23 August 2023) (edit) (undo)
 
(14 intermediate revisions not shown.)
Line 1: Line 1:
-
[[Image:1xkw.gif|left|200px]]<br /><applet load="1xkw" size="450" color="white" frame="true" align="right" spinBox="true"
 
-
caption="1xkw, resolution 2.0&Aring;" />
 
-
'''Pyochelin outer membrane receptor FptA from Pseudomonas aeruginosa'''<br />
 
-
==Overview==
+
==Pyochelin outer membrane receptor FptA from Pseudomonas aeruginosa==
-
Pyochelin is a siderophore and virulence factor common to Burkholderia, cepacia and several Pseudomonas strains. We describe at 2.0 A resolution, the crystal structure of the pyochelin outer membrane receptor FptA bound, to the iron-pyochelin isolated from Pseudomonas aeruginosa. One pyochelin, molecule bound to iron is found in the protein structure, providing the, first three-dimensional structure at the atomic level of this siderophore., The pyochelin molecule provides a tetra-dentate coordination of iron, while the remaining bi-dentate coordination is ensured by another molecule, not specifically recognized by the protein. The overall structure of the, pyochelin receptor is typical of the TonB-dependent transporter, superfamily, which uses the proton motive force from the cytoplasmic, membrane through the TonB-ExbB-ExbD energy transducing complex to, transport ferric ions across the bacterial outer membrane: a transmembrane, 22 beta-stranded barrel occluded by a N-terminal domain that contains a, mixed four-stranded beta-sheet. The N-terminal TonB box is disordered in, two crystal forms, and loop L8 is found to point towards the, iron-pyochelin complex, suggesting that the receptor is in a, transport-competent conformation.
+
<StructureSection load='1xkw' size='340' side='right'caption='[[1xkw]], [[Resolution|resolution]] 2.00&Aring;' scene=''>
 +
== Structural highlights ==
 +
<table><tr><td colspan='2'>[[1xkw]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Pseudomonas_aeruginosa Pseudomonas aeruginosa]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1XKW OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1XKW FirstGlance]. <br>
 +
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2&#8491;</td></tr>
 +
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=188:PYOCHELIN+FE(III)'>188</scene>, <scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=LDA:LAURYL+DIMETHYLAMINE-N-OXIDE'>LDA</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
 +
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1xkw FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1xkw OCA], [https://pdbe.org/1xkw PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1xkw RCSB], [https://www.ebi.ac.uk/pdbsum/1xkw PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1xkw ProSAT]</span></td></tr>
 +
</table>
 +
== Function ==
 +
[https://www.uniprot.org/uniprot/FPTA_PSEAE FPTA_PSEAE] High-affinity outer membrane receptor required for the transport of Fe(3+)-pyochelin.
 +
== Evolutionary Conservation ==
 +
[[Image:Consurf_key_small.gif|200px|right]]
 +
Check<jmol>
 +
<jmolCheckbox>
 +
<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/xk/1xkw_consurf.spt"</scriptWhenChecked>
 +
<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
 +
<text>to colour the structure by Evolutionary Conservation</text>
 +
</jmolCheckbox>
 +
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1xkw ConSurf].
 +
<div style="clear:both"></div>
 +
<div style="background-color:#fffaf0;">
 +
== Publication Abstract from PubMed ==
 +
Pyochelin is a siderophore and virulence factor common to Burkholderia cepacia and several Pseudomonas strains. We describe at 2.0 A resolution the crystal structure of the pyochelin outer membrane receptor FptA bound to the iron-pyochelin isolated from Pseudomonas aeruginosa. One pyochelin molecule bound to iron is found in the protein structure, providing the first three-dimensional structure at the atomic level of this siderophore. The pyochelin molecule provides a tetra-dentate coordination of iron, while the remaining bi-dentate coordination is ensured by another molecule not specifically recognized by the protein. The overall structure of the pyochelin receptor is typical of the TonB-dependent transporter superfamily, which uses the proton motive force from the cytoplasmic membrane through the TonB-ExbB-ExbD energy transducing complex to transport ferric ions across the bacterial outer membrane: a transmembrane 22 beta-stranded barrel occluded by a N-terminal domain that contains a mixed four-stranded beta-sheet. The N-terminal TonB box is disordered in two crystal forms, and loop L8 is found to point towards the iron-pyochelin complex, suggesting that the receptor is in a transport-competent conformation.
-
==About this Structure==
+
Crystal structure at high resolution of ferric-pyochelin and its membrane receptor FptA from Pseudomonas aeruginosa.,Cobessi D, Celia H, Pattus F J Mol Biol. 2005 Sep 30;352(4):893-904. PMID:16139844<ref>PMID:16139844</ref>
-
1XKW is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Pseudomonas_aeruginosa Pseudomonas aeruginosa] with SO4, LDA, 188 and EDO as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1XKW OCA].
+
-
==Reference==
+
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
-
Crystal structure at high resolution of ferric-pyochelin and its membrane receptor FptA from Pseudomonas aeruginosa., Cobessi D, Celia H, Pattus F, J Mol Biol. 2005 Sep 30;352(4):893-904. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=16139844 16139844]
+
</div>
 +
<div class="pdbe-citations 1xkw" style="background-color:#fffaf0;"></div>
 +
== References ==
 +
<references/>
 +
__TOC__
 +
</StructureSection>
 +
[[Category: Large Structures]]
[[Category: Pseudomonas aeruginosa]]
[[Category: Pseudomonas aeruginosa]]
-
[[Category: Single protein]]
+
[[Category: Celia H]]
-
[[Category: Celia, H.]]
+
[[Category: Cobessi D]]
-
[[Category: Cobessi, D.]]
+
[[Category: Pattus F]]
-
[[Category: Pattus, F.]]
+
-
[[Category: 188]]
+
-
[[Category: EDO]]
+
-
[[Category: LDA]]
+
-
[[Category: SO4]]
+
-
[[Category: tonb dependent receptor]]
+
-
 
+
-
''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Wed Nov 21 06:08:05 2007''
+

Current revision

Pyochelin outer membrane receptor FptA from Pseudomonas aeruginosa

PDB ID 1xkw

Drag the structure with the mouse to rotate

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/1xkw"
Personal tools