1jl1

From Proteopedia

(Difference between revisions)
Jump to: navigation, search
Current revision (08:42, 16 August 2023) (edit) (undo)
 
(11 intermediate revisions not shown.)
Line 1: Line 1:
-
{{Seed}}
 
-
[[Image:1jl1.png|left|200px]]
 
-
<!--
+
==D10A E. coli ribonuclease HI==
-
The line below this paragraph, containing "STRUCTURE_1jl1", creates the "Structure Box" on the page.
+
<StructureSection load='1jl1' size='340' side='right'caption='[[1jl1]], [[Resolution|resolution]] 1.30&Aring;' scene=''>
-
You may change the PDB parameter (which sets the PDB file loaded into the applet)
+
== Structural highlights ==
-
or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
+
<table><tr><td colspan='2'>[[1jl1]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1JL1 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1JL1 FirstGlance]. <br>
-
or leave the SCENE parameter empty for the default display.
+
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.3&#8491;</td></tr>
-
-->
+
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1jl1 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1jl1 OCA], [https://pdbe.org/1jl1 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1jl1 RCSB], [https://www.ebi.ac.uk/pdbsum/1jl1 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1jl1 ProSAT]</span></td></tr>
-
{{STRUCTURE_1jl1| PDB=1jl1 | SCENE= }}
+
</table>
 +
== Function ==
 +
[https://www.uniprot.org/uniprot/RNH_ECOLI RNH_ECOLI] Endonuclease that specifically degrades the RNA of RNA-DNA hybrids. RNase H participates in DNA replication; it helps to specify the origin of genomic replication by suppressing initiation at origins other than the oriC locus; along with the 5'-3' exonuclease of pol1, it removes RNA primers from the Okazaki fragments of lagging strand synthesis; and it defines the origin of replication for ColE1-type plasmids by specific cleavage of an RNA preprimer.[HAMAP-Rule:MF_00042]
 +
== Evolutionary Conservation ==
 +
[[Image:Consurf_key_small.gif|200px|right]]
 +
Check<jmol>
 +
<jmolCheckbox>
 +
<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/jl/1jl1_consurf.spt"</scriptWhenChecked>
 +
<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
 +
<text>to colour the structure by Evolutionary Conservation</text>
 +
</jmolCheckbox>
 +
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1jl1 ConSurf].
 +
<div style="clear:both"></div>
 +
<div style="background-color:#fffaf0;">
 +
== Publication Abstract from PubMed ==
 +
Escherichia coli RNase HI is a well-characterized model system for protein folding and stability. Controlling protein stability is critical for both natural proteins and for the development of engineered proteins that function under extreme conditions. We have used native-state hydrogen exchange on a variant containing the stabilizing mutation Asp10 to alanine in order to determine its residue-specific stabilities. On average, the DeltaG(unf) value for each residue was increased by 2-3 kcal/mol, resulting in a lower relative population of partially unfolded forms. Though increased in stability by a uniform factor, D10A shows a distribution of stabilities in its secondary structural units that is similar to that of E. coli RNase H, but not the closely related protein from Thermus thermophilus. Hence, the simple mutation used to stabilize the enzyme does not recreate the balance of conformational flexibility evolved in the thermophilic protein.
-
===D10A E. coli ribonuclease HI===
+
Native-state energetics of a thermostabilized variant of ribonuclease HI.,Goedken ER, Marqusee S J Mol Biol. 2001 Dec 7;314(4):863-71. PMID:11734003<ref>PMID:11734003</ref>
 +
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
 +
</div>
 +
<div class="pdbe-citations 1jl1" style="background-color:#fffaf0;"></div>
-
<!--
+
==See Also==
-
The line below this paragraph, {{ABSTRACT_PUBMED_11734003}}, adds the Publication Abstract to the page
+
*[[Ribonuclease 3D structures|Ribonuclease 3D structures]]
-
(as it appears on PubMed at http://www.pubmed.gov), where 11734003 is the PubMed ID number.
+
== References ==
-
-->
+
<references/>
-
{{ABSTRACT_PUBMED_11734003}}
+
__TOC__
-
 
+
</StructureSection>
-
==About this Structure==
+
-
1JL1 is a 1 chain structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1JL1 OCA].
+
-
 
+
-
==Reference==
+
-
<ref group="xtra">PMID:11734003</ref><references group="xtra"/>
+
[[Category: Escherichia coli]]
[[Category: Escherichia coli]]
-
[[Category: Ribonuclease H]]
+
[[Category: Large Structures]]
-
[[Category: Goedken, E R.]]
+
[[Category: Goedken ER]]
-
[[Category: Marqusee, S.]]
+
[[Category: Marqusee S]]
-
[[Category: Cooperativity]]
+
-
[[Category: Hydrogen exchange]]
+
-
[[Category: Protein stability]]
+
-
[[Category: Rnase hi]]
+
-
[[Category: Thermostability]]
+
-
 
+
-
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Feb 16 12:51:26 2009''
+

Current revision

D10A E. coli ribonuclease HI

PDB ID 1jl1

Drag the structure with the mouse to rotate

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/1jl1"
Personal tools