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1xp5

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(New page: 200px<br /><applet load="1xp5" size="450" color="white" frame="true" align="right" spinBox="true" caption="1xp5, resolution 3.0&Aring;" /> '''Structure Of The (Sr)...)
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[[Image:1xp5.gif|left|200px]]<br /><applet load="1xp5" size="450" color="white" frame="true" align="right" spinBox="true"
 
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caption="1xp5, resolution 3.0&Aring;" />
 
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'''Structure Of The (Sr)Ca2+-ATPase E2-AlF4- Form'''<br />
 
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==Overview==
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==Structure Of The (Sr)Ca2+-ATPase E2-AlF4- Form==
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P-type ATPases extract energy by hydrolysis of adenosine triphosphate, (ATP) in two steps, formation and breakdown of a covalent phosphoenzyme, intermediate. This process drives active transport and countertransport of, the cation pumps. We have determined the crystal structure of rabbit, sarcoplasmic reticulum Ca2+ adenosine triphosphatase in complex with, aluminum fluoride, which mimics the transition state of hydrolysis of the, counterion-bound (protonated) phosphoenzyme. On the basis of structural, analysis and biochemical data, we find this form to represent an occluded, state of the proton counterions. Hydrolysis is catalyzed by the conserved, Thr-Gly-Glu-Ser motif, and it exploits an associative nucleophilic, reaction mechanism of the same type as phosphoryl transfer from ATP. On, this basis, we propose a general mechanism of occluded transition states, of Ca2+ transport and H+ countertransport coupled to phosphorylation and, dephosphorylation, respectively.
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<StructureSection load='1xp5' size='340' side='right'caption='[[1xp5]], [[Resolution|resolution]] 3.00&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[1xp5]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Oryctolagus_cuniculus Oryctolagus cuniculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1XP5 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1XP5 FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 3&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ALF:TETRAFLUOROALUMINATE+ION'>ALF</scene>, <scene name='pdbligand=K:POTASSIUM+ION'>K</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=TG1:OCTANOIC+ACID+[3S-[3ALPHA,+3ABETA,+4ALPHA,+6BETA,+6ABETA,+7BETA,+8ALPHA(Z),+9BALPHA]]-6-(ACETYLOXY)-2,3,-3A,4,5,6,6A,7,8,9B-DECAHYDRO-3,3A-DIHYDROXY-3,6,9-TRIMETHYL-8-[(2-METHYL-1-OXO-2-BUTENYL)OX+Y]-2-OXO-4-(1-OXOBUTOXY)-AZULENO[4,5-B]FURAN-7-YL+ESTER'>TG1</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1xp5 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1xp5 OCA], [https://pdbe.org/1xp5 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1xp5 RCSB], [https://www.ebi.ac.uk/pdbsum/1xp5 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1xp5 ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/AT2A1_RABIT AT2A1_RABIT] This magnesium-dependent enzyme catalyzes the hydrolysis of ATP coupled with the translocation of calcium from the cytosol to the sarcoplasmic reticulum lumen. Contributes to calcium sequestration involved in muscular excitation/contraction (By similarity).
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/xp/1xp5_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1xp5 ConSurf].
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<div style="clear:both"></div>
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==About this Structure==
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==See Also==
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1XP5 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Oryctolagus_cuniculus Oryctolagus cuniculus] with MG, ALF, K and TG1 as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/Calcium-transporting_ATPase Calcium-transporting ATPase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.6.3.8 3.6.3.8] Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1XP5 OCA].
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*[[ATPase 3D structures|ATPase 3D structures]]
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__TOC__
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==Reference==
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</StructureSection>
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Dephosphorylation of the calcium pump coupled to counterion occlusion., Olesen C, Sorensen TL, Nielsen RC, Moller JV, Nissen P, Science. 2004 Dec 24;306(5705):2251-5. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=15618517 15618517]
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[[Category: Large Structures]]
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[[Category: Calcium-transporting ATPase]]
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[[Category: Oryctolagus cuniculus]]
[[Category: Oryctolagus cuniculus]]
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[[Category: Single protein]]
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[[Category: Moller JV]]
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[[Category: Moller, J.V.]]
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[[Category: Nielsen RC]]
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[[Category: Nielsen, R.C.]]
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[[Category: Nissen P]]
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[[Category: Nissen, P.]]
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[[Category: Olesen C]]
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[[Category: Olesen, C.]]
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[[Category: Sorensen TLS]]
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[[Category: Sorensen, T.L.S.]]
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[[Category: ALF]]
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[[Category: K]]
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[[Category: MG]]
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[[Category: TG1]]
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[[Category: aluminium fluoride]]
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[[Category: ca2+-atpase]]
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[[Category: membrane protein]]
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[[Category: p-type atpase]]
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''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Wed Nov 21 06:14:41 2007''
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Current revision

Structure Of The (Sr)Ca2+-ATPase E2-AlF4- Form

PDB ID 1xp5

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