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1ojx

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{{Seed}}
 
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[[Image:1ojx.png|left|200px]]
 
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==Crystal structure of an Archaeal fructose 1,6-bisphosphate aldolase==
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The line below this paragraph, containing "STRUCTURE_1ojx", creates the "Structure Box" on the page.
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<StructureSection load='1ojx' size='340' side='right'caption='[[1ojx]], [[Resolution|resolution]] 1.90&Aring;' scene=''>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>[[1ojx]] is a 10 chain structure with sequence from [https://en.wikipedia.org/wiki/Thermoproteus_tenax Thermoproteus tenax]. The February 2004 RCSB PDB [https://pdb.rcsb.org/pdb/static.do?p=education_discussion/molecule_of_the_month/index.html Molecule of the Month] feature on ''The Glycolytic Enzymes'' by David S. Goodsell is [https://dx.doi.org/10.2210/rcsb_pdb/mom_2004_2 10.2210/rcsb_pdb/mom_2004_2]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1OJX OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1OJX FirstGlance]. <br>
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or leave the SCENE parameter empty for the default display.
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.9&#8491;</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1ojx FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1ojx OCA], [https://pdbe.org/1ojx PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1ojx RCSB], [https://www.ebi.ac.uk/pdbsum/1ojx PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1ojx ProSAT]</span></td></tr>
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{{STRUCTURE_1ojx| PDB=1ojx | SCENE= }}
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/ALF1_THETK ALF1_THETK] Catalyzes the reversible cleavage of fructose 1,6-bisphosphate (FBP) to glyceraldehyde 3-phosphate (GAP) and dihydroxyacetone phosphate (DHAP).
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/oj/1ojx_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1ojx ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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Fructose-1,6-bisphosphate aldolase (FBPA) catalyzes the reversible cleavage of fructose 1,6-bisphosphate to glyceraldehyde 3-phosphate and dihydroxyacetone phosphate in the glycolytic pathway. FBPAs from archaeal organisms have recently been identified and characterized as a divergent family of proteins. Here, we report the first crystal structure of an archaeal FBPA at 1.9-A resolution. The structure of this 280-kDa protein complex was determined using single wavelength anomalous dispersion followed by 10-fold non-crystallographic symmetry averaging and refined to an R-factor of 14.9% (Rfree 17.9%). The protein forms a dimer of pentamers, consisting of subunits adopting the ubiquitous (betaalpha)8 barrel fold. Additionally, a crystal structure of the archaeal FBPA covalently bound to dihydroxyacetone phosphate was solved at 2.1-A resolution. Comparison of the active site residues with those of classical FBPAs, which share no significant sequence identity but display the same overall fold, reveals a common ancestry between these two families of FBPAs. Structural comparisons, furthermore, establish an evolutionary link to the triosephosphate isomerases, a superfamily hitherto considered independent from the superfamily of aldolases.
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===CRYSTAL STRUCTURE OF AN ARCHAEAL FRUCTOSE 1,6-BISPHOSPHATE ALDOLASE===
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Crystal structure of an archaeal class I aldolase and the evolution of (betaalpha)8 barrel proteins.,Lorentzen E, Pohl E, Zwart P, Stark A, Russell RB, Knura T, Hensel R, Siebers B J Biol Chem. 2003 Nov 21;278(47):47253-60. Epub 2003 Aug 26. PMID:12941964<ref>PMID:12941964</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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<div class="pdbe-citations 1ojx" style="background-color:#fffaf0;"></div>
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==See Also==
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The line below this paragraph, {{ABSTRACT_PUBMED_12941964}}, adds the Publication Abstract to the page
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*[[Aldolase 3D structures|Aldolase 3D structures]]
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(as it appears on PubMed at http://www.pubmed.gov), where 12941964 is the PubMed ID number.
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== References ==
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<references/>
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{{ABSTRACT_PUBMED_12941964}}
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__TOC__
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</StructureSection>
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==About this Structure==
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[[Category: Large Structures]]
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1OJX is a 10 chains structure of sequences from [http://en.wikipedia.org/wiki/Thermoproteus_tenax Thermoproteus tenax]. The February 2004 RCSB PDB [http://pdb.rcsb.org/pdb/static.do?p=education_discussion/molecule_of_the_month/index.html Molecule of the Month] feature on ''The Glycolytic Enzymes'' by David S. Goodsell is [http://dx.doi.org/10.2210/rcsb_pdb/mom_2004_2 10.2210/rcsb_pdb/mom_2004_2]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1OJX OCA].
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[[Category: RCSB PDB Molecule of the Month]]
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==Reference==
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<ref group="xtra">PMID:12941964</ref><references group="xtra"/>
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[[Category: Fructose-bisphosphate aldolase]]
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[[Category: The Glycolytic Enzymes]]
[[Category: The Glycolytic Enzymes]]
[[Category: Thermoproteus tenax]]
[[Category: Thermoproteus tenax]]
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[[Category: Hensel, R.]]
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[[Category: Hensel R]]
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[[Category: Lorentzen, E.]]
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[[Category: Lorentzen E]]
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[[Category: Pohl, E.]]
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[[Category: Pohl E]]
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[[Category: Siebers, B.]]
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[[Category: Siebers B]]
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[[Category: Stark, A.]]
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[[Category: Stark A]]
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[[Category: Zwart, P.]]
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[[Category: Zwart P]]
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[[Category: 6-bisphosphate]]
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[[Category: Aldolase]]
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[[Category: Archaeal]]
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[[Category: Fructose 1]]
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[[Category: Glycolytic]]
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[[Category: Lyase]]
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[[Category: Tim barrel]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Feb 16 13:09:15 2009''
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Current revision

Crystal structure of an Archaeal fructose 1,6-bisphosphate aldolase

PDB ID 1ojx

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