2h84

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{{Seed}}
 
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[[Image:2h84.png|left|200px]]
 
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==Crystal Structure of the C-terminal Type III Polyketide Synthase (PKS III) Domain of 'Steely1' (a Type I/III PKS Hybrid from Dictyostelium)==
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The line below this paragraph, containing "STRUCTURE_2h84", creates the "Structure Box" on the page.
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<StructureSection load='2h84' size='340' side='right'caption='[[2h84]], [[Resolution|resolution]] 2.90&Aring;' scene=''>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>[[2h84]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Dictyostelium_discoideum_AX4 Dictyostelium discoideum AX4]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2H84 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2H84 FirstGlance]. <br>
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or leave the SCENE parameter empty for the default display.
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.9&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=P6G:HEXAETHYLENE+GLYCOL'>P6G</scene></td></tr>
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{{STRUCTURE_2h84| PDB=2h84 | SCENE= }}
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2h84 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2h84 OCA], [https://pdbe.org/2h84 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2h84 RCSB], [https://www.ebi.ac.uk/pdbsum/2h84 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2h84 ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/PKS1_DICDI PKS1_DICDI] Probable polyketide synthase (By similarity). Produces only acylpyrones; in vitro.<ref>PMID:16906151</ref>
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/h8/2h84_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2h84 ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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Differentiation-inducing factors (DIFs) are well known to modulate formation of distinct communal cell types from identical Dictyostelium discoideum amoebas, but DIF biosynthesis remains obscure. We report complimentary in vivo and in vitro experiments identifying one of two approximately 3,000-residue D. discoideum proteins, termed 'steely', as responsible for biosynthesis of the DIF acylphloroglucinol scaffold. Steely proteins possess six catalytic domains homologous to metazoan type I fatty acid synthases (FASs) but feature an iterative type III polyketide synthase (PKS) in place of the expected FAS C-terminal thioesterase used to off load fatty acid products. This new domain arrangement likely facilitates covalent transfer of steely N-terminal acyl products directly to the C-terminal type III PKS active sites, which catalyze both iterative polyketide extension and cyclization. The crystal structure of a steely C-terminal domain confirms conservation of the homodimeric type III PKS fold. These findings suggest new bioengineering strategies for expanding the scope of fatty acid and polyketide biosynthesis.
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===Crystal Structure of the C-terminal Type III Polyketide Synthase (PKS III) Domain of 'Steely1' (a Type I/III PKS Hybrid from Dictyostelium)===
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Biosynthesis of Dictyostelium discoideum differentiation-inducing factor by a hybrid type I fatty acid-type III polyketide synthase.,Austin MB, Saito T, Bowman ME, Haydock S, Kato A, Moore BS, Kay RR, Noel JP Nat Chem Biol. 2006 Sep;2(9):494-502. Epub 2006 Aug 13. PMID:16906151<ref>PMID:16906151</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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The line below this paragraph, {{ABSTRACT_PUBMED_16906151}}, adds the Publication Abstract to the page
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<div class="pdbe-citations 2h84" style="background-color:#fffaf0;"></div>
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(as it appears on PubMed at http://www.pubmed.gov), where 16906151 is the PubMed ID number.
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== References ==
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<references/>
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{{ABSTRACT_PUBMED_16906151}}
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__TOC__
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</StructureSection>
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==About this Structure==
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[[Category: Dictyostelium discoideum AX4]]
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2H84 is a 2 chains structure of sequences from [http://en.wikipedia.org/wiki/Dictyostelium_discoideum Dictyostelium discoideum]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2H84 OCA].
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[[Category: Large Structures]]
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[[Category: Austin MB]]
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==Reference==
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[[Category: Bowman ME]]
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<ref group="xtra">PMID:16906151</ref><references group="xtra"/>
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[[Category: Haydock S]]
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[[Category: Dictyostelium discoideum]]
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[[Category: Kato A]]
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[[Category: Austin, M B.]]
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[[Category: Kay RR]]
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[[Category: Bowman, M E.]]
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[[Category: Moore BS]]
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[[Category: Haydock, S.]]
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[[Category: Noel JP]]
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[[Category: Kato, A.]]
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[[Category: Saito T]]
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[[Category: Kay, R R.]]
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[[Category: Moore, B S.]]
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[[Category: Noel, J P.]]
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[[Category: Saito, T.]]
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[[Category: Chalcone-stilbene synthase superfamily]]
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[[Category: Fa]]
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[[Category: Pk]]
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[[Category: Thiolase-fold]]
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[[Category: Type i fatty acid synthase]]
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[[Category: Type i pk]]
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[[Category: Type iii polyketide synthase]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Feb 16 13:30:22 2009''
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Current revision

Crystal Structure of the C-terminal Type III Polyketide Synthase (PKS III) Domain of 'Steely1' (a Type I/III PKS Hybrid from Dictyostelium)

PDB ID 2h84

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