1xs0

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(New page: 200px<br /><applet load="1xs0" size="450" color="white" frame="true" align="right" spinBox="true" caption="1xs0, resolution 1.58&Aring;" /> '''Structure of the E. ...)
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[[Image:1xs0.jpg|left|200px]]<br /><applet load="1xs0" size="450" color="white" frame="true" align="right" spinBox="true"
 
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caption="1xs0, resolution 1.58&Aring;" />
 
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'''Structure of the E. coli Ivy protein'''<br />
 
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==Overview==
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==Structure of the E. coli Ivy protein==
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The complete nucleotide sequences of over 37 microbial and three eukaryote, genomes are already publicly available, and more sequencing is in, progress. Despite this accumulation of data, newly sequenced microbial, genomes continue to reveal up to 50% of functionally uncharacterized, "anonymous" genes. A majority of these anonymous proteins have homologues, in other organisms, whereas the rest exhibit no clear similarity to any, other sequence in the data bases. This set of unique, apparently, species-specific, sequences are referred to as ORFans. The biochemical and, structural analysis of ORFan gene products is of both evolutionary and, functional interest. Here we report the cloning and expression of, Escherichia coli ORFan ykfE gene and the functional characterization of, the encoded protein. Under physiological conditions, the protein is a, homodimer with a strong affinity for C-type lysozyme, as revealed by, co-purification and co-crystallization. Activity measurements and, fluorescence studies demonstrated that the YkfE gene product is a potent, C-type lysozyme inhibitor (K(i) approximately 1 nm). To denote this newly, assigned function, ykfE has now been registered under the new gene name, Ivy (inhibitor of vertebrate lysozyme) at the E. coli genetic stock, center.
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<StructureSection load='1xs0' size='340' side='right'caption='[[1xs0]], [[Resolution|resolution]] 1.58&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[1xs0]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli_K-12 Escherichia coli K-12]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1XS0 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1XS0 FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.58&#8491;</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1xs0 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1xs0 OCA], [https://pdbe.org/1xs0 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1xs0 RCSB], [https://www.ebi.ac.uk/pdbsum/1xs0 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1xs0 ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/IVY_ECOLI IVY_ECOLI] Strong inhibitor of lysozyme C.
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/xs/1xs0_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview03.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1xs0 ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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Part of an ancestral bactericidal system, vertebrate C-type lysozyme targets the peptidoglycan moiety of bacterial cell walls. We report the crystal structure of a protein inhibitor of C-type lysozyme, the Escherichia coli Ivy protein, alone and in complex with hen egg white lysozyme. Ivy exhibits a novel fold in which a protruding five-residue loop appears essential to its inhibitory effect. This feature guided the identification of Ivy orthologues in other Gram-negative bacteria. The structure of the evolutionary distant Pseudomonas aeruginosa Ivy orthologue was also determined in complex with hen egg white lysozyme, and its antilysozyme activity was confirmed. Ivy expression protects porous cell-wall E. coli mutants from the lytic effect of lysozyme, suggesting that it is a response against the permeabilizing effects of the innate vertebrate immune system. As such, Ivy acts as a virulence factor for a number of Gram-negative bacteria-infecting vertebrates.
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==About this Structure==
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Structure and evolution of the Ivy protein family, unexpected lysozyme inhibitors in Gram-negative bacteria.,Abergel C, Monchois V, Byrne D, Chenivesse S, Lembo F, Lazzaroni JC, Claverie JM Proc Natl Acad Sci U S A. 2007 Apr 10;104(15):6394-9. Epub 2007 Apr 3. PMID:17405861<ref>PMID:17405861</ref>
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1XS0 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1XS0 OCA].
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==Reference==
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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Escherichia coli ykfE ORFan gene encodes a potent inhibitor of C-type lysozyme., Monchois V, Abergel C, Sturgis J, Jeudy S, Claverie JM, J Biol Chem. 2001 May 25;276(21):18437-41. Epub 2001 Feb 23. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=11278658 11278658]
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</div>
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[[Category: Escherichia coli]]
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<div class="pdbe-citations 1xs0" style="background-color:#fffaf0;"></div>
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[[Category: Single protein]]
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== References ==
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[[Category: Abergel, C.]]
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<references/>
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[[Category: Byrn, D.]]
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__TOC__
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[[Category: Claverie, J.M.]]
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</StructureSection>
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[[Category: Lazzaroni, J.C.]]
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[[Category: Escherichia coli K-12]]
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[[Category: Monchois, V.]]
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[[Category: Large Structures]]
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[[Category: alpha beta fold]]
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[[Category: Abergel C]]
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[[Category: dimer]]
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[[Category: Byrn D]]
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[[Category: Claverie JM]]
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''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Wed Nov 21 06:18:27 2007''
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[[Category: Lazzaroni JC]]
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[[Category: Monchois V]]

Current revision

Structure of the E. coli Ivy protein

PDB ID 1xs0

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