2jet

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{{Seed}}
 
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[[Image:2jet.png|left|200px]]
 
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==Crystal structure of a trypsin-like mutant (S189D , A226G) chymotrypsin.==
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The line below this paragraph, containing "STRUCTURE_2jet", creates the "Structure Box" on the page.
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<StructureSection load='2jet' size='340' side='right'caption='[[2jet]], [[Resolution|resolution]] 2.20&Aring;' scene=''>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>[[2jet]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Rattus_norvegicus Rattus norvegicus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2JET OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2JET FirstGlance]. <br>
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or leave the SCENE parameter empty for the default display.
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.2&#8491;</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2jet FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2jet OCA], [https://pdbe.org/2jet PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2jet RCSB], [https://www.ebi.ac.uk/pdbsum/2jet PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2jet ProSAT]</span></td></tr>
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{{STRUCTURE_2jet| PDB=2jet | SCENE= }}
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/CTRB1_RAT CTRB1_RAT]
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/je/2jet_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview03.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2jet ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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The crystal structure of the S189D+A226G rat chymotrypsin-B mutant has been determined at 2.2 A resolution. This mutant is the most trypsin-like mutant so far in the line of chymotrypsin-to-trypsin conversions, aiming for a more complete understanding of the structural basis of substrate specificity in pancreatic serine proteases. A226G caused significant rearrangements relative to S189D chymotrypsin, allowing an internal conformation of Asp189 which is close to that in trypsin. Serious distortions remain, however, in the activation domain, including zymogen-like features. The pH-profile of activity suggests that the conformation of the S1-site of the mutant is influenced also by the P1 residue of the substrate.
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===CRYSTAL STRUCTURE OF A TRYPSIN-LIKE MUTANT (S189D, A226G) CHYMOTRYPSIN.===
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The Crystal Structure of a Trypsin-like Mutant Chymotrypsin: The Role of Position 226 in the Activity and Specificity of S189D Chymotrypsin.,Jelinek B, Katona G, Fodor K, Venekei I, Graf L Protein J. 2007 Sep 6;. PMID:17805946<ref>PMID:17805946</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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<div class="pdbe-citations 2jet" style="background-color:#fffaf0;"></div>
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==See Also==
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The line below this paragraph, {{ABSTRACT_PUBMED_17805946}}, adds the Publication Abstract to the page
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*[[Chymotrypsin 3D structures|Chymotrypsin 3D structures]]
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(as it appears on PubMed at http://www.pubmed.gov), where 17805946 is the PubMed ID number.
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== References ==
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<references/>
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{{ABSTRACT_PUBMED_17805946}}
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__TOC__
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</StructureSection>
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==About this Structure==
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[[Category: Large Structures]]
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2JET is a 3 chains structure of sequences from [http://en.wikipedia.org/wiki/Rattus_norvegicus Rattus norvegicus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2JET OCA].
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==Reference==
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<ref group="xtra">PMID:17805946</ref><references group="xtra"/>
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[[Category: Chymotrypsin]]
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[[Category: Rattus norvegicus]]
[[Category: Rattus norvegicus]]
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[[Category: Fodor, K.]]
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[[Category: Fodor K]]
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[[Category: Graf, L.]]
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[[Category: Graf L]]
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[[Category: Jelinek, B.]]
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[[Category: Jelinek B]]
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[[Category: Katona, G.]]
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[[Category: Katona G]]
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[[Category: Venekei, I.]]
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[[Category: Venekei I]]
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[[Category: Digestion]]
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[[Category: Hydrolase]]
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[[Category: Protease]]
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[[Category: Protein engineering]]
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[[Category: Serine protease]]
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[[Category: Substrate specificity]]
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[[Category: Zymogen]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Feb 16 13:31:15 2009''
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Current revision

Crystal structure of a trypsin-like mutant (S189D , A226G) chymotrypsin.

PDB ID 2jet

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