1zyr

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{{Seed}}
 
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[[Image:1zyr.png|left|200px]]
 
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==Structure of Thermus thermophilus RNA polymerase holoenzyme in complex with the antibiotic streptolydigin==
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The line below this paragraph, containing "STRUCTURE_1zyr", creates the "Structure Box" on the page.
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<StructureSection load='1zyr' size='340' side='right'caption='[[1zyr]], [[Resolution|resolution]] 3.00&Aring;' scene=''>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>[[1zyr]] is a 12 chain structure with sequence from [https://en.wikipedia.org/wiki/Thermus_thermophilus Thermus thermophilus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1ZYR OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1ZYR FirstGlance]. <br>
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or leave the SCENE parameter empty for the default display.
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 3&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=STD:STREPTOLYDIGIN'>STD</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
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{{STRUCTURE_1zyr| PDB=1zyr | SCENE= }}
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1zyr FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1zyr OCA], [https://pdbe.org/1zyr PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1zyr RCSB], [https://www.ebi.ac.uk/pdbsum/1zyr PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1zyr ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/RPOA_THET8 RPOA_THET8] DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates.
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/zy/1zyr_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview03.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1zyr ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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We define the target, mechanism, and structural basis of inhibition of bacterial RNA polymerase (RNAP) by the tetramic acid antibiotic streptolydigin (Stl). Stl binds to a site adjacent to but not overlapping the RNAP active center and stabilizes an RNAP-active-center conformational state with a straight-bridge helix. The results provide direct support for the proposals that alternative straight-bridge-helix and bent-bridge-helix RNAP-active-center conformations exist and that cycling between straight-bridge-helix and bent-bridge-helix RNAP-active-center conformations is required for RNAP function. The results set bounds on models for RNAP function and suggest strategies for design of novel antibacterial agents.
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===Structure of Thermus thermophilus RNA polymerase holoenzyme in complex with the antibiotic streptolydigin===
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Inhibition of bacterial RNA polymerase by streptolydigin: stabilization of a straight-bridge-helix active-center conformation.,Tuske S, Sarafianos SG, Wang X, Hudson B, Sineva E, Mukhopadhyay J, Birktoft JJ, Leroy O, Ismail S, Clark AD Jr, Dharia C, Napoli A, Laptenko O, Lee J, Borukhov S, Ebright RH, Arnold E Cell. 2005 Aug 26;122(4):541-52. PMID:16122422<ref>PMID:16122422</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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<div class="pdbe-citations 1zyr" style="background-color:#fffaf0;"></div>
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==See Also==
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The line below this paragraph, {{ABSTRACT_PUBMED_16122422}}, adds the Publication Abstract to the page
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*[[RNA polymerase 3D structures|RNA polymerase 3D structures]]
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(as it appears on PubMed at http://www.pubmed.gov), where 16122422 is the PubMed ID number.
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== References ==
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<references/>
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{{ABSTRACT_PUBMED_16122422}}
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__TOC__
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</StructureSection>
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==About this Structure==
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[[Category: Large Structures]]
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1ZYR is a 12 chains structure of sequences from [http://en.wikipedia.org/wiki/Thermus_thermophilus Thermus thermophilus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1ZYR OCA].
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==Reference==
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<ref group="xtra">PMID:16122422</ref><references group="xtra"/>
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[[Category: DNA-directed RNA polymerase]]
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[[Category: Thermus thermophilus]]
[[Category: Thermus thermophilus]]
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[[Category: Arnold, E.]]
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[[Category: Arnold E]]
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[[Category: Birktoft, J J.]]
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[[Category: Birktoft JJ]]
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[[Category: Borukhov, S.]]
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[[Category: Borukhov S]]
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[[Category: Clark, A D.]]
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[[Category: Clark AD]]
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[[Category: Dharia, C.]]
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[[Category: Dharia C]]
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[[Category: Ebright, R H.]]
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[[Category: Ebright RH]]
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[[Category: Hudson, B.]]
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[[Category: Hudson B]]
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[[Category: Ismail, S.]]
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[[Category: Ismail S]]
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[[Category: Laptenko, O.]]
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[[Category: Laptenko O]]
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[[Category: Lee, J.]]
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[[Category: Lee J]]
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[[Category: Leroy, O.]]
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[[Category: Leroy O]]
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[[Category: Mukhopadhyay, J.]]
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[[Category: Mukhopadhyay J]]
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[[Category: Napoli, A.]]
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[[Category: Napoli A]]
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[[Category: Sarafianos, S G.]]
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[[Category: Sarafianos SG]]
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[[Category: Sineva, E.]]
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[[Category: Sineva E]]
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[[Category: Tuske, S.]]
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[[Category: Tuske S]]
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[[Category: Wang, X.]]
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[[Category: Wang X]]
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[[Category: Holoenzyme]]
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[[Category: Rna polymerase]]
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[[Category: Streptolydigin]]
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[[Category: Transcription]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Feb 16 14:00:44 2009''
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Current revision

Structure of Thermus thermophilus RNA polymerase holoenzyme in complex with the antibiotic streptolydigin

PDB ID 1zyr

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