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2nuu

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{{Seed}}
 
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[[Image:2nuu.png|left|200px]]
 
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==Regulating the Escherichia coli ammonia channel: the crystal structure of the AmtB-GlnK complex==
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The line below this paragraph, containing "STRUCTURE_2nuu", creates the "Structure Box" on the page.
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<StructureSection load='2nuu' size='340' side='right'caption='[[2nuu]], [[Resolution|resolution]] 2.50&Aring;' scene=''>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>[[2nuu]] is a 12 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2NUU OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2NUU FirstGlance]. <br>
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or leave the SCENE parameter empty for the default display.
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.5&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ADP:ADENOSINE-5-DIPHOSPHATE'>ADP</scene></td></tr>
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{{STRUCTURE_2nuu| PDB=2nuu | SCENE= }}
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2nuu FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2nuu OCA], [https://pdbe.org/2nuu PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2nuu RCSB], [https://www.ebi.ac.uk/pdbsum/2nuu PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2nuu ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/AMTB_ECOLI AMTB_ECOLI] Involved in the uptake of ammonia.
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/nu/2nuu_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2nuu ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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Amt proteins are ubiquitous channels for the conduction of ammonia in archaea, eubacteria, fungi, and plants. In Escherichia coli, previous studies have indicated that binding of the PII signal transduction protein GlnK to the ammonia channel AmtB regulates the channel thereby controlling ammonium influx in response to the intracellular nitrogen status. Here, we describe the crystal structure of the complex between AmtB and GlnK at a resolution of 2.5 A. This structure of PII in a complex with one of its targets reveals physiologically relevant conformations of both AmtB and GlnK. GlnK interacts with AmtB almost exclusively via a long surface loop containing Y51 (T-loop), the tip of which inserts deeply into the cytoplasmic pore exit, blocking ammonia conduction. Y51 of GlnK is also buried in the pore exit, explaining why uridylylation of this residue prevents complex formation.
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===Regulating the Escherichia coli ammonia channel: the crystal structure of the AmtB-GlnK complex===
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The crystal structure of the Escherichia coli AmtB-GlnK complex reveals how GlnK regulates the ammonia channel.,Conroy MJ, Durand A, Lupo D, Li XD, Bullough PA, Winkler FK, Merrick M Proc Natl Acad Sci U S A. 2007 Jan 23;104(4):1213-8. Epub 2007 Jan 12. PMID:17220269<ref>PMID:17220269</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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<div class="pdbe-citations 2nuu" style="background-color:#fffaf0;"></div>
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==See Also==
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The line below this paragraph, {{ABSTRACT_PUBMED_17220269}}, adds the Publication Abstract to the page
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*[[Ion channels 3D structures|Ion channels 3D structures]]
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(as it appears on PubMed at http://www.pubmed.gov), where 17220269 is the PubMed ID number.
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== References ==
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<references/>
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{{ABSTRACT_PUBMED_17220269}}
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__TOC__
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</StructureSection>
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==About this Structure==
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2NUU is a 12 chains structure of sequences from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2NUU OCA].
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==Reference==
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<ref group="xtra">PMID:17220269</ref><references group="xtra"/>
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[[Category: Escherichia coli]]
[[Category: Escherichia coli]]
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[[Category: Bullough, P A.]]
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[[Category: Large Structures]]
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[[Category: Conroy, M J.]]
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[[Category: Bullough PA]]
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[[Category: Durand, A.]]
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[[Category: Conroy MJ]]
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[[Category: Li, X D.]]
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[[Category: Durand A]]
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[[Category: Lupo, D.]]
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[[Category: Li X-D]]
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[[Category: Merrick, M.]]
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[[Category: Lupo D]]
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[[Category: Winkler, F K.]]
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[[Category: Merrick M]]
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[[Category: Ammonia transport]]
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[[Category: Winkler FK]]
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[[Category: Membrane protein complex]]
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[[Category: Nitrogen regulation]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Feb 16 14:42:44 2009''
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Current revision

Regulating the Escherichia coli ammonia channel: the crystal structure of the AmtB-GlnK complex

PDB ID 2nuu

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