2eww

From Proteopedia

(Difference between revisions)
Jump to: navigation, search
Current revision (07:57, 30 October 2024) (edit) (undo)
 
(10 intermediate revisions not shown.)
Line 1: Line 1:
-
{{Seed}}
 
-
[[Image:2eww.png|left|200px]]
 
-
<!--
+
==Crystal Structure of the Pilus Retraction Motor PilT and Bound ATP==
-
The line below this paragraph, containing "STRUCTURE_2eww", creates the "Structure Box" on the page.
+
<StructureSection load='2eww' size='340' side='right'caption='[[2eww]], [[Resolution|resolution]] 3.20&Aring;' scene=''>
-
You may change the PDB parameter (which sets the PDB file loaded into the applet)
+
== Structural highlights ==
-
or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
+
<table><tr><td colspan='2'>[[2eww]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Aquifex_aeolicus Aquifex aeolicus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2EWW OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2EWW FirstGlance]. <br>
-
or leave the SCENE parameter empty for the default display.
+
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 3.2&#8491;</td></tr>
-
-->
+
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ATP:ADENOSINE-5-TRIPHOSPHATE'>ATP</scene>, <scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr>
-
{{STRUCTURE_2eww| PDB=2eww | SCENE= }}
+
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2eww FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2eww OCA], [https://pdbe.org/2eww PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2eww RCSB], [https://www.ebi.ac.uk/pdbsum/2eww PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2eww ProSAT]</span></td></tr>
 +
</table>
 +
== Function ==
 +
[https://www.uniprot.org/uniprot/O66950_AQUAE O66950_AQUAE]
 +
== Evolutionary Conservation ==
 +
[[Image:Consurf_key_small.gif|200px|right]]
 +
Check<jmol>
 +
<jmolCheckbox>
 +
<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ew/2eww_consurf.spt"</scriptWhenChecked>
 +
<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview03.spt</scriptWhenUnchecked>
 +
<text>to colour the structure by Evolutionary Conservation</text>
 +
</jmolCheckbox>
 +
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2eww ConSurf].
 +
<div style="clear:both"></div>
 +
<div style="background-color:#fffaf0;">
 +
== Publication Abstract from PubMed ==
 +
PilT is a hexameric ATPase required for bacterial type IV pilus retraction and surface motility. Crystal structures of ADP- and ATP-bound Aquifex aeolicus PilT at 2.8 and 3.2 A resolution show N-terminal PAS-like and C-terminal RecA-like ATPase domains followed by a set of short C-terminal helices. The hexamer is formed by extensive polar subunit interactions between the ATPase core of one monomer and the N-terminal domain of the next. An additional structure captures a nonsymmetric PilT hexamer in which approach of invariant arginines from two subunits to the bound nucleotide forms an enzymatically competent active site. A panel of pilT mutations highlights the importance of the arginines, the PAS-like domain, the polar subunit interface, and the C-terminal helices for retraction. We present a model for ATP binding leading to dramatic PilT domain motions, engagement of the arginine wire, and subunit communication in this hexameric motor. Our conclusions apply to the entire type II/IV secretion ATPase family.
-
===Crystal Structure of the Pilus Retraction Motor PilT and Bound ATP===
+
Crystal structures of the pilus retraction motor PilT suggest large domain movements and subunit cooperation drive motility.,Satyshur KA, Worzalla GA, Meyer LS, Heiniger EK, Aukema KG, Misic AM, Forest KT Structure. 2007 Mar;15(3):363-76. PMID:17355871<ref>PMID:17355871</ref>
-
 
+
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
-
<!--
+
</div>
-
The line below this paragraph, {{ABSTRACT_PUBMED_17355871}}, adds the Publication Abstract to the page
+
<div class="pdbe-citations 2eww" style="background-color:#fffaf0;"></div>
-
(as it appears on PubMed at http://www.pubmed.gov), where 17355871 is the PubMed ID number.
+
== References ==
-
-->
+
<references/>
-
{{ABSTRACT_PUBMED_17355871}}
+
__TOC__
-
 
+
</StructureSection>
-
==About this Structure==
+
-
2EWW is a 1 chain structure of sequence from [http://en.wikipedia.org/wiki/Aquifex_aeolicus Aquifex aeolicus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2EWW OCA].
+
-
 
+
-
==Reference==
+
-
<ref group="xtra">PMID:17355871</ref><ref group="xtra">PMID:15103158</ref><references group="xtra"/>
+
[[Category: Aquifex aeolicus]]
[[Category: Aquifex aeolicus]]
-
[[Category: Forest, K T.]]
+
[[Category: Large Structures]]
-
[[Category: Satyshur, K A.]]
+
[[Category: Forest KT]]
-
[[Category: Atpase]]
+
[[Category: Satyshur KA]]
-
[[Category: Hexameric pilt]]
+
-
[[Category: Pilus retraction motor]]
+
-
 
+
-
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Feb 16 15:19:29 2009''
+

Current revision

Crystal Structure of the Pilus Retraction Motor PilT and Bound ATP

PDB ID 2eww

Drag the structure with the mouse to rotate

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/2eww"
Personal tools