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1bkj

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(New page: 200px<br /> <applet load="1bkj" size="450" color="white" frame="true" align="right" spinBox="true" caption="1bkj, resolution 1.8&Aring;" /> '''NADPH:FMN OXIDOREDUC...)
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[[Image:1bkj.gif|left|200px]]<br />
 
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<applet load="1bkj" size="450" color="white" frame="true" align="right" spinBox="true"
 
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caption="1bkj, resolution 1.8&Aring;" />
 
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'''NADPH:FMN OXIDOREDUCTASE FROM VIBRIO HARVEYI'''<br />
 
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==Overview==
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==NADPH:FMN OXIDOREDUCTASE FROM VIBRIO HARVEYI==
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We report the structure of an NADPH:FMN oxidoreductase (flavin reductase, P) that is involved in bioluminescence by providing reduced FMN to, luciferase. The 1.8 A crystal structure of flavin reductase P from Vibrio, harveyi was solved by multiple isomorphous replacement and reveals that, the enzyme is a unique dimer of interlocking subunits, with 9352 A2 of, surface area buried in the dimer interface. Each subunit comprises two, domains. The first domain consists of a four-stranded antiparallel, beta-sheet flanked by helices on either side. The second domain reaches, out from one subunit and embraces the other subunit and is responsible for, interlocking the two subunits. Our structure explains why flavin reductase, P is specific for FMN as cofactor. FMN is recognized and tightly bound ... [[http://ispc.weizmann.ac.il/pmbin/getpm?8885832 (full description)]]
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<StructureSection load='1bkj' size='340' side='right'caption='[[1bkj]], [[Resolution|resolution]] 1.80&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[1bkj]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Vibrio_harveyi Vibrio harveyi]. This structure supersedes the now removed PDB entry [http://oca.weizmann.ac.il/oca-bin/send-pdb?obs=1&id=1cum 1cum]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1BKJ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1BKJ FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.8&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=FMN:FLAVIN+MONONUCLEOTIDE'>FMN</scene>, <scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1bkj FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1bkj OCA], [https://pdbe.org/1bkj PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1bkj RCSB], [https://www.ebi.ac.uk/pdbsum/1bkj PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1bkj ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/FRP_VIBHA FRP_VIBHA] Involved in bioluminescence. It is a good supplier of reduced flavin mononucleotide (FMNH2) to the bioluminescence reaction.
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/bk/1bkj_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1bkj ConSurf].
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<div style="clear:both"></div>
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==About this Structure==
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==See Also==
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1BKJ is a [[http://en.wikipedia.org/wiki/Single_protein Single protein]] structure of sequence from [[http://en.wikipedia.org/wiki/Vibrio_harveyi Vibrio harveyi]] with PO4 and FMN as [[http://en.wikipedia.org/wiki/ligands ligands]]. This structure superseeds the now removed PDB entry 1CUM. Active as [[http://en.wikipedia.org/wiki/ ]], with EC number [[http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.6.8.1 1.6.8.1]]. Full crystallographic information is available from [[http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1BKJ OCA]].
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*[[Flavin reductase|Flavin reductase]]
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__TOC__
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==Reference==
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</StructureSection>
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Flavin reductase P: structure of a dimeric enzyme that reduces flavin., Tanner JJ, Lei B, Tu SC, Krause KL, Biochemistry. 1996 Oct 22;35(42):13531-9. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=8885832 8885832]
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[[Category: Large Structures]]
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[[Category: Single protein]]
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[[Category: Vibrio harveyi]]
[[Category: Vibrio harveyi]]
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[[Category: Krause, K.L.]]
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[[Category: Krause KL]]
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[[Category: Lei, B.]]
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[[Category: Lei B]]
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[[Category: TU, S.C.]]
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[[Category: TU S-C]]
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[[Category: Tanner, J.J.]]
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[[Category: Tanner JJ]]
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[[Category: FMN]]
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[[Category: PO4]]
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[[Category: flavoprotein]]
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[[Category: luminescence]]
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[[Category: nadp]]
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[[Category: oxidoreductase]]
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''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Mon Oct 29 21:32:56 2007''
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Current revision

NADPH:FMN OXIDOREDUCTASE FROM VIBRIO HARVEYI

PDB ID 1bkj

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