2k48

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[[Image:2k48.png|left|200px]]
 
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==NMR Structure of the N-terminal Coiled Coil Domain of the Andes Hantavirus Nucleocapsid Protein==
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<StructureSection load='2k48' size='340' side='right'caption='[[2k48]]' scene=''>
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>[[2k48]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Andes_orthohantavirus Andes orthohantavirus]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2K48 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2K48 FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2k48 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2k48 OCA], [https://pdbe.org/2k48 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2k48 RCSB], [https://www.ebi.ac.uk/pdbsum/2k48 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2k48 ProSAT]</span></td></tr>
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{{STRUCTURE_2k48| PDB=2k48 | SCENE= }}
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/NCAP_ANDV NCAP_ANDV] Encapsidates the genome protecting it from nucleases (Probable). The encapsidated genomic RNA is termed the nucleocapsid (NC) and serves as template for transcription and replication (Probable). The nucleocapsid has a left-handed helical structure (By similarity). As a trimer, specifically binds and acts as a chaperone to unwind the panhandle structure formed by the viral RNA (vRNA) termini (By similarity). Involved in the transcription and replication initiation of vRNA by mediating primer annealing (By similarity). Plays a role in cap snatching by sequestering capped RNAs in P bodies for use by the viral RdRp during transcription initiation (By similarity). Substitutes for the cellular cap-binding complex (eIF4F) to preferentially facilitate the translation of capped mRNAs (By similarity). Initiates the translation by specifically binding to the cap and 40S ribosomal subunit (By similarity). Prevents the viral glycoprotein N (Gn) from autophagy-dependent breakdown maybe by blocking autophagosome formation (By similarity). Inhibits host EIF2AK2/PKR dimerization to prevent PKR-induced translational shutdown in cells and thus the activation of the antiviral state (PubMed:25410857). Inhibits IFN signaling responses directed by the dsRNA sensors RIGI and IFIH1/MDA5, probably by interacting with host E3 ubiquitin ligase TRIM21 (PubMed:24549848). As a consequence, TBK1-directed IRF3 phosphorylation and TBK1 autophosphorylation are inhibited (PubMed:24549848, PubMed:30867297). Also displays sequence-unspecific DNA endonuclease activity (By similarity).[UniProtKB:P05133][UniProtKB:Q89462]<ref>PMID:24549848</ref> <ref>PMID:25410857</ref> <ref>PMID:30867297</ref>
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/k4/2k48_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2k48 ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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The hantaviruses are emerging infectious viruses that in humans can cause a cardiopulmonary syndrome or a hemorrhagic fever with renal syndrome. The nucleocapsid (N) is the most abundant viral protein, and during viral assembly, the N protein forms trimers and packages the viral RNA genome. Here, we report the NMR structure of the N-terminal domain (residues 1-74, called N1-74) of the Andes hantavirus N protein. N1-74 forms two long helices (alpha1 and alpha2) that intertwine into a coiled coil domain. The conserved hydrophobic residues at the helix alpha1-alpha2 interface stabilize the coiled coil; however, there are many conserved surface residues whose function is not known. Site-directed mutagenesis, CD spectroscopy, and immunocytochemistry reveal that a point mutation in the conserved basic surface formed by Arg22 or Lys26 lead to antibody recognition based on the subcellular localization of the N protein. Thus, Arg22 and Lys26 are likely involved in a conformational change or molecular recognition when the N protein is trafficked from the cytoplasm to the Golgi, the site of viral assembly and maturation.
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===NMR Structure of the N-terminal Coiled Coil Domain of the Andes Hantavirus Nucleocapsid Protein===
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NMR structure of the N-terminal coiled coil domain of the Andes hantavirus nucleocapsid protein.,Wang Y, Boudreaux DM, Estrada DF, Egan CW, St Jeor SC, De Guzman RN J Biol Chem. 2008 Oct 17;283(42):28297-304. Epub 2008 Aug 7. PMID:18687679<ref>PMID:18687679</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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<div class="pdbe-citations 2k48" style="background-color:#fffaf0;"></div>
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==See Also==
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The line below this paragraph, {{ABSTRACT_PUBMED_18687679}}, adds the Publication Abstract to the page
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*[[Nucleoprotein 3D structures|Nucleoprotein 3D structures]]
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(as it appears on PubMed at http://www.pubmed.gov), where 18687679 is the PubMed ID number.
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== References ==
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<references/>
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{{ABSTRACT_PUBMED_18687679}}
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__TOC__
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</StructureSection>
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==About this Structure==
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[[Category: Andes orthohantavirus]]
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2K48 is a 1 chain structure of sequence from [http://en.wikipedia.org/wiki/Andes_virus Andes virus]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2K48 OCA].
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[[Category: Large Structures]]
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[[Category: Boudreaux DM]]
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==Reference==
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[[Category: De Guzman RN]]
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<ref group="xtra">PMID:18687679</ref><references group="xtra"/>
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[[Category: Egan CW]]
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[[Category: Andes virus]]
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[[Category: Estrada DF]]
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[[Category: Boudreaux, D M.]]
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[[Category: St Jeor SC]]
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[[Category: Egan, C W.]]
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[[Category: Wang Y]]
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[[Category: Estrada, D F.]]
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[[Category: Guzman, R N.De.]]
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[[Category: Jeor, S C.St.]]
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[[Category: Wang, Y.]]
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[[Category: Viral nucleoprotein]]
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[[Category: Viral protein]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Feb 16 15:59:45 2009''
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Current revision

NMR Structure of the N-terminal Coiled Coil Domain of the Andes Hantavirus Nucleocapsid Protein

PDB ID 2k48

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