1yhb

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(New page: 200px<br /><applet load="1yhb" size="450" color="white" frame="true" align="right" spinBox="true" caption="1yhb, resolution 2.2&Aring;" /> '''CRYSTAL STRUCTURES OF...)
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[[Image:1yhb.jpg|left|200px]]<br /><applet load="1yhb" size="450" color="white" frame="true" align="right" spinBox="true"
 
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caption="1yhb, resolution 2.2&Aring;" />
 
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'''CRYSTAL STRUCTURES OF Y41H AND Y41F MUTANTS OF GENE V PROTEIN FROM FF PHAGE SUGGEST POSSIBLE PROTEIN-PROTEIN INTERACTIONS IN GVP-SSDNA COMPLEX'''<br />
 
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==Overview==
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==CRYSTAL STRUCTURES OF Y41H AND Y41F MUTANTS OF GENE V PROTEIN FROM FF PHAGE SUGGEST POSSIBLE PROTEIN-PROTEIN INTERACTIONS IN GVP-SSDNA COMPLEX==
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Gene V protein (GVP) encoded by the filamentous phage Ff (M13, fl, fd) is, a homodimeric protein of 87 amino acids that binds to single-stranded DNA, (ssDNA) nonspecifically and cooperatively. The structure (monoclinic C2, form) of the wild-type protein has been determined and refined at 1.8-A, resolution [Skinner et al. (1994) Proc. Natl. Acad. Sci. U.S.A. 91, 2071-2075]. The monomer structure consists of a somewhat distorted, five-stranded beta-barrel core with three prominent loops: a DNA-binding, loop, a dyad loop, and a dimer contact loop. The amino acid residue at, position 41 plays an important role in the dimer-dimer interactions of the, protein-ssDNA complex. Two Y41 mutant structures have been studied by, X-ray crystallography. The Y41F GVP structure has been refined to an, R-factor of 0.180 at 2.2-A resolution and is very similar to the wild-type, (wt) structure (rmsd of all C alpha atoms = 0.30 A). In contrast, Y41H GVP, forms a new crystal lattice in the space group P2(1)2(1)2(1) with a =, 77.18 A, b = 84.17 A, and c = 28.62 A. Its structure has been solved by, the molecular replacement method and refined to an R-factor of 0.170 at, 2.5-A resolution. The two monomers of Y41H are crystallographically, independent, and their structures remain similar to wt-GVP but with, significant differences, particularly in the DNA-binding hairpin region., In both crystals, the loop (residues 36-43) that contains the Y41 residue, is involved in the crystal dimer packings but in a different manner. The, dimer-dimer contacts found in the wt-GVP crystal may be important for GVP, aggregation in the absence of DNA. In the presence of DNA, the dimer-dimer, contacts may switch to the type found in the Y41H crystal, allowing the, GVP-ssDNA complex to form cooperatively. A model of the complex, consistent with existing biochemical and biophysical data, has been, constructed from those crystal packing data.
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<StructureSection load='1yhb' size='340' side='right'caption='[[1yhb]], [[Resolution|resolution]] 2.20&Aring;' scene=''>
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== Structural highlights ==
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==About this Structure==
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<table><tr><td colspan='2'>[[1yhb]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Enterobacteria_phage_f1 Enterobacteria phage f1]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1YHB OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1YHB FirstGlance]. <br>
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1YHB is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Bacteriophage_f1 Bacteriophage f1]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1YHB OCA].
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.2&#8491;</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1yhb FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1yhb OCA], [https://pdbe.org/1yhb PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1yhb RCSB], [https://www.ebi.ac.uk/pdbsum/1yhb PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1yhb ProSAT]</span></td></tr>
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==Reference==
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</table>
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Crystal structures of Y41H and Y41F mutants of gene V protein from Ff phage suggest possible protein-protein interactions in the GVP-ssDNA complex., Guan Y, Zhang H, Konings RN, Hilbers CW, Terwilliger TC, Wang AH, Biochemistry. 1994 Jun 28;33(25):7768-78. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=8011642 8011642]
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== Function ==
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[[Category: Bacteriophage f1]]
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[https://www.uniprot.org/uniprot/G5P_BPF1 G5P_BPF1] Binds to DNA in a highly cooperative manner without pronounced sequence specificity. During synthesis of the single-stranded (progeny) viral DNA, prevents the conversion into the double-stranded replicative form. G5P is displaced by the capsid protein G8P during phage assembly on the inner bacterial membrane (By similarity).
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[[Category: Single protein]]
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== Evolutionary Conservation ==
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[[Category: Guan, Y.]]
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[[Image:Consurf_key_small.gif|200px|right]]
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[[Category: Hilbers, C.W.]]
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Check<jmol>
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[[Category: Konings, R.N.H.]]
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<jmolCheckbox>
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[[Category: Terwilliger, T.C.]]
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/yh/1yhb_consurf.spt"</scriptWhenChecked>
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[[Category: Wang, A.H.J.]]
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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[[Category: Zhang, H.]]
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<text>to colour the structure by Evolutionary Conservation</text>
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[[Category: dna-binding protein]]
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1yhb ConSurf].
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''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Wed Nov 21 06:46:29 2007''
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<div style="clear:both"></div>
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__TOC__
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</StructureSection>
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[[Category: Enterobacteria phage f1]]
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[[Category: Large Structures]]
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[[Category: Guan Y]]
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[[Category: Hilbers CW]]
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[[Category: Konings RNH]]
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[[Category: Terwilliger TC]]
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[[Category: Wang AH-J]]
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[[Category: Zhang H]]

Current revision

CRYSTAL STRUCTURES OF Y41H AND Y41F MUTANTS OF GENE V PROTEIN FROM FF PHAGE SUGGEST POSSIBLE PROTEIN-PROTEIN INTERACTIONS IN GVP-SSDNA COMPLEX

PDB ID 1yhb

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