2z5o

From Proteopedia

(Difference between revisions)
Jump to: navigation, search
Current revision (13:22, 1 November 2023) (edit) (undo)
 
(8 intermediate revisions not shown.)
Line 1: Line 1:
-
{{Seed}}
 
-
[[Image:2z5o.png|left|200px]]
 
-
<!--
+
==Complex of Transportin 1 with JKTBP NLS==
-
The line below this paragraph, containing "STRUCTURE_2z5o", creates the "Structure Box" on the page.
+
<StructureSection load='2z5o' size='340' side='right'caption='[[2z5o]], [[Resolution|resolution]] 3.20&Aring;' scene=''>
-
You may change the PDB parameter (which sets the PDB file loaded into the applet)
+
== Structural highlights ==
-
or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
+
<table><tr><td colspan='2'>[[2z5o]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2Z5O OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2Z5O FirstGlance]. <br>
-
or leave the SCENE parameter empty for the default display.
+
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 3.2&#8491;</td></tr>
-
-->
+
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2z5o FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2z5o OCA], [https://pdbe.org/2z5o PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2z5o RCSB], [https://www.ebi.ac.uk/pdbsum/2z5o PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2z5o ProSAT]</span></td></tr>
-
{{STRUCTURE_2z5o| PDB=2z5o | SCENE= }}
+
</table>
 +
== Function ==
 +
[https://www.uniprot.org/uniprot/TNPO1_HUMAN TNPO1_HUMAN] Functions in nuclear protein import as nuclear transport receptor. Serves as receptor for nuclear localization signals (NLS) in cargo substrates. Is thought to mediate docking of the importin/substrate complex to the nuclear pore complex (NPC) through binding to nucleoporin and the complex is subsequently translocated through the pore by an energy requiring, Ran-dependent mechanism. At the nucleoplasmic side of the NPC, Ran binds to the importin, the importin/substrate complex dissociates and importin is re-exported from the nucleus to the cytoplasm where GTP hydrolysis releases Ran. The directionality of nuclear import is thought to be conferred by an asymmetric distribution of the GTP- and GDP-bound forms of Ran between the cytoplasm and nucleus (By similarity). Involved in nuclear import of M9-containing proteins. In vitro, binds directly to the M9 region of the heterogeneous nuclear ribonucleoproteins (hnRNP), A1 and A2 and mediates their nuclear import. Appears also to be involved in hnRNP A1/A2 nuclear export. Mediates the nuclear import of ribosomal proteins RPL23A, RPS7 and RPL5. Binds to a beta-like import receptor binding (BIB) domain of RPL23A. In vitro, mediates nuclear import of H2A, H2B, H3 and H4 histones, and SRP19. In case of HIV-1 infection, binds and mediates the nuclear import of HIV-1 Rev. Mediates nuclear import of ADAR/ADAR1 (isoform 5) in a RanGTP-dependent manner.<ref>PMID:8986607</ref> <ref>PMID:9687515</ref> <ref>PMID:11682607</ref> <ref>PMID:19124606</ref>
 +
== Evolutionary Conservation ==
 +
[[Image:Consurf_key_small.gif|200px|right]]
 +
Check<jmol>
 +
<jmolCheckbox>
 +
<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/z5/2z5o_consurf.spt"</scriptWhenChecked>
 +
<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
 +
<text>to colour the structure by Evolutionary Conservation</text>
 +
</jmolCheckbox>
 +
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2z5o ConSurf].
 +
<div style="clear:both"></div>
 +
<div style="background-color:#fffaf0;">
 +
== Publication Abstract from PubMed ==
 +
Transportin 1 (Trn1) is a transport receptor that transports substrates from the cytoplasm to the nucleus through nuclear pore complexes by recognizing nuclear localization signals (NLSs). Here we describe four crystal structures of human Trn1 in a substrate-free form as well as in the complex with three NLSs (hnRNP D, JKTBP, and TAP, respectively). Our data have revealed that (1) Trn1 has two sites for binding NLSs, one with high affinity (site A) and one with low affinity (site B), and NLS interaction at site B controls overall binding affinity for Trn1; (2) Trn1 recognizes the NLSs at site A followed by conformational change at site B to interact with the NLSs; and (3) a long flexible loop, characteristic of Trn1, interacts with site B, thereby displacing transport substrate in the nucleus. These studies provide deep understanding of substrate recognition and dissociation by Trn1 in import pathways.
-
===Complex of Transportin 1 with JKTBP NLS===
+
Structural basis for substrate recognition and dissociation by human transportin 1.,Imasaki T, Shimizu T, Hashimoto H, Hidaka Y, Kose S, Imamoto N, Yamada M, Sato M Mol Cell. 2007 Oct 12;28(1):57-67. PMID:17936704<ref>PMID:17936704</ref>
 +
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
 +
</div>
 +
<div class="pdbe-citations 2z5o" style="background-color:#fffaf0;"></div>
-
<!--
+
==See Also==
-
The line below this paragraph, {{ABSTRACT_PUBMED_17936704}}, adds the Publication Abstract to the page
+
*[[Importin 3D structures|Importin 3D structures]]
-
(as it appears on PubMed at http://www.pubmed.gov), where 17936704 is the PubMed ID number.
+
*[[Nucleoprotein 3D structures|Nucleoprotein 3D structures]]
-
-->
+
== References ==
-
{{ABSTRACT_PUBMED_17936704}}
+
<references/>
-
 
+
__TOC__
-
==About this Structure==
+
</StructureSection>
-
2Z5O is a 2 chains structure of sequences from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2Z5O OCA].
+
-
 
+
-
==Reference==
+
-
<ref group="xtra">PMID:17936704</ref><references group="xtra"/>
+
[[Category: Homo sapiens]]
[[Category: Homo sapiens]]
-
[[Category: Hashimoto, H.]]
+
[[Category: Large Structures]]
-
[[Category: Hidaka, Y.]]
+
[[Category: Hashimoto H]]
-
[[Category: Imamoto, N.]]
+
[[Category: Hidaka Y]]
-
[[Category: Imasaki, T.]]
+
[[Category: Imamoto N]]
-
[[Category: Kose, S.]]
+
[[Category: Imasaki T]]
-
[[Category: Sato, M.]]
+
[[Category: Kose S]]
-
[[Category: Shimizu, T.]]
+
[[Category: Sato M]]
-
[[Category: Yamada, M.]]
+
[[Category: Shimizu T]]
-
[[Category: Exportin]]
+
[[Category: Yamada M]]
-
[[Category: Hnrnp]]
+
-
[[Category: Importin]]
+
-
[[Category: Karyopherin]]
+
-
[[Category: Ne]]
+
-
[[Category: Nl]]
+
-
[[Category: Nuclear transport]]
+
-
[[Category: Nucleocytoplasmic]]
+
-
[[Category: Transport protein/rna binding protein complex]]
+
-
 
+
-
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Feb 16 16:31:36 2009''
+

Current revision

Complex of Transportin 1 with JKTBP NLS

PDB ID 2z5o

Drag the structure with the mouse to rotate

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/2z5o"
Personal tools