1yke

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(New page: 200px<br /><applet load="1yke" size="450" color="white" frame="true" align="right" spinBox="true" caption="1yke, resolution 3.3&Aring;" /> '''Structure of the medi...)
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[[Image:1yke.gif|left|200px]]<br /><applet load="1yke" size="450" color="white" frame="true" align="right" spinBox="true"
 
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caption="1yke, resolution 3.3&Aring;" />
 
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'''Structure of the mediator MED7/MED21 subcomplex'''<br />
 
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==Overview==
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==Structure of the mediator MED7/MED21 subcomplex==
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The Mediator of transcriptional regulation is the central coactivator that, enables a response of RNA polymerase II (Pol II) to activators and, repressors. We present the 3.0-A crystal structure of a highly conserved, part of the Mediator, the MED7.MED21 (Med7.Srb7) heterodimer. The, structure is very extended, spanning one-third of the Mediator length and, almost the diameter of Pol II. It shows a four-helix bundle domain and a, coiled-coil protrusion connected by a flexible hinge. Four putative, protein binding sites on the surface allow for assembly of the Mediator, middle module and for binding of the conserved subunit MED6, which is, shown to bridge to the Mediator head module. A flexible MED6 bridge and, the MED7.MED21 hinge could account for changes in overall Mediator, structure upon binding to Pol II or activators. Our results support the, idea that transcription regulation involves conformational changes within, the general machinery.
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<StructureSection load='1yke' size='340' side='right'caption='[[1yke]], [[Resolution|resolution]] 3.30&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[1yke]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1YKE OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1YKE FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 3.3&#8491;</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1yke FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1yke OCA], [https://pdbe.org/1yke PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1yke RCSB], [https://www.ebi.ac.uk/pdbsum/1yke PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1yke ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/MED7_YEAST MED7_YEAST] Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. The Mediator complex, having a compact conformation in its free form, is recruited to promoters by direct interactions with regulatory proteins and serves for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors. The Mediator complex unfolds to an extended conformation and partially surrounds RNA polymerase II, specifically interacting with the unphosphorylated form of the C-terminal domain (CTD) of RNA polymerase II. The Mediator complex dissociates from the RNA polymerase II holoenzyme and stays at the promoter when transcriptional elongation begins.<ref>PMID:11555651</ref> <ref>PMID:16076843</ref> <ref>PMID:16263706</ref>
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/yk/1yke_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1yke ConSurf].
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<div style="clear:both"></div>
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==About this Structure==
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==See Also==
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1YKE is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1YKE OCA].
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*[[Mediator|Mediator]]
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== References ==
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==Reference==
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<references/>
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A conserved mediator hinge revealed in the structure of the MED7.MED21 (Med7.Srb7) heterodimer., Baumli S, Hoeppner S, Cramer P, J Biol Chem. 2005 May 6;280(18):18171-8. Epub 2005 Feb 14. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=15710619 15710619]
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__TOC__
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[[Category: Protein complex]]
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</StructureSection>
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[[Category: Large Structures]]
[[Category: Saccharomyces cerevisiae]]
[[Category: Saccharomyces cerevisiae]]
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[[Category: Baumli, S.]]
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[[Category: Baumli S]]
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[[Category: Cramer, P.]]
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[[Category: Cramer P]]
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[[Category: Hoeppner, S.]]
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[[Category: Hoeppner S]]
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[[Category: gene regulation]]
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''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Wed Nov 21 06:51:35 2007''
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Current revision

Structure of the mediator MED7/MED21 subcomplex

PDB ID 1yke

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