2c3t

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{{Seed}}
 
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[[Image:2c3t.png|left|200px]]
 
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==Human glutathione-S-transferase T1-1, W234R mutant, apo form==
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The line below this paragraph, containing "STRUCTURE_2c3t", creates the "Structure Box" on the page.
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<StructureSection load='2c3t' size='340' side='right'caption='[[2c3t]], [[Resolution|resolution]] 2.40&Aring;' scene=''>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>[[2c3t]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2C3T OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2C3T FirstGlance]. <br>
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or leave the SCENE parameter empty for the default display.
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.4&#8491;</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2c3t FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2c3t OCA], [https://pdbe.org/2c3t PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2c3t RCSB], [https://www.ebi.ac.uk/pdbsum/2c3t PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2c3t ProSAT]</span></td></tr>
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{{STRUCTURE_2c3t| PDB=2c3t | SCENE= }}
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/GSTT1_HUMAN GSTT1_HUMAN] Conjugation of reduced glutathione to a wide number of exogenous and endogenous hydrophobic electrophiles. Acts on 1,2-epoxy-3-(4-nitrophenoxy)propane, phenethylisothiocyanate 4-nitrobenzyl chloride and 4-nitrophenethyl bromide. Displays glutathione peroxidase activity with cumene hydroperoxide.<ref>PMID:16298388</ref> <ref>PMID:20097269</ref>
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/c3/2c3t_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2c3t ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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The crystal structures of wild-type human theta class glutathione-S-transferase (GST) T1-1 and its W234R mutant, where Trp234 was replaced by Arg, were solved both in the presence and absence of S-hexyl-glutathione. The W234R mutant was of interest due to its previously observed enhanced catalytic activity compared to the wild-type enzyme. GST T1-1 from rat and mouse naturally contain Arg in position 234, with correspondingly high catalytic efficiency. The overall structure of GST T1-1 is similar to that of GST T2-2, as expected from their 53% sequence identity at the protein level. Wild-type GST T1-1 has the side-chain of Trp234 occupying a significant portion of the active site. This bulky residue prevents efficient binding of both glutathione and hydrophobic substrates through steric hindrance. The wild-type GST T1-1 crystal structure, obtained from co-crystallization experiments with glutathione and its derivatives, showed no electron density for the glutathione ligand. However, the structure of GST T1-1 mutant W234R showed clear electron density for S-hexyl-glutathione after co-crystallization. In contrast to Trp234 in the wild-type structure, the side-chain of Arg234 in the mutant does not occupy any part of the substrate-binding site. Instead, Arg234 is pointing in a different direction and, in addition, interacts with the carboxylate group of glutathione. These findings explain our earlier observation that the W234R mutant has a markedly improved catalytic activity with most substrates tested to date compared to the wild-type enzyme. GST T1-1 catalyzes detoxication reactions as well as reactions that result in toxic products, and our findings therefore suggest that humans have gained an evolutionary advantage by a partially disabled active site.
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===HUMAN GLUTATHIONE-S-TRANSFERASE T1-1, W234R MUTANT, APO FORM===
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Structural basis of the suppressed catalytic activity of wild-type human glutathione transferase T1-1 compared to its W234R mutant.,Tars K, Larsson AK, Shokeer A, Olin B, Mannervik B, Kleywegt GJ J Mol Biol. 2006 Jan 6;355(1):96-105. Epub 2005 Nov 8. PMID:16298388<ref>PMID:16298388</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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<div class="pdbe-citations 2c3t" style="background-color:#fffaf0;"></div>
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==See Also==
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The line below this paragraph, {{ABSTRACT_PUBMED_16298388}}, adds the Publication Abstract to the page
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*[[Glutathione S-transferase 3D structures|Glutathione S-transferase 3D structures]]
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(as it appears on PubMed at http://www.pubmed.gov), where 16298388 is the PubMed ID number.
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== References ==
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<references/>
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{{ABSTRACT_PUBMED_16298388}}
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__TOC__
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</StructureSection>
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==About this Structure==
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2C3T is a 4 chains structure of sequences from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2C3T OCA].
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==Reference==
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<ref group="xtra">PMID:16298388</ref><references group="xtra"/>
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[[Category: Glutathione transferase]]
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[[Category: Homo sapiens]]
[[Category: Homo sapiens]]
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[[Category: Kleywegt, G J.]]
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[[Category: Large Structures]]
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[[Category: Larsson, A K.]]
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[[Category: Kleywegt GJ]]
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[[Category: Mannervik, B.]]
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[[Category: Larsson A-K]]
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[[Category: Olin, B.]]
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[[Category: Mannervik B]]
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[[Category: Shokeer, A.]]
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[[Category: Olin B]]
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[[Category: Tars, K.]]
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[[Category: Shokeer A]]
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[[Category: Glutathione]]
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[[Category: Tars K]]
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[[Category: Glutathione transferase]]
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[[Category: Polymorphism]]
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[[Category: T1-1]]
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[[Category: Transferase]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Feb 16 16:55:43 2009''
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Current revision

Human glutathione-S-transferase T1-1, W234R mutant, apo form

PDB ID 2c3t

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