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1bqy

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{{Seed}}
 
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[[Image:1bqy.png|left|200px]]
 
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==Plasminogen activator (TSV-PA) from snake venom==
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The line below this paragraph, containing "STRUCTURE_1bqy", creates the "Structure Box" on the page.
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<StructureSection load='1bqy' size='340' side='right'caption='[[1bqy]], [[Resolution|resolution]] 2.50&Aring;' scene=''>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>[[1bqy]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Trimeresurus_stejnegeri Trimeresurus stejnegeri]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1BQY OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1BQY FirstGlance]. <br>
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or leave the SCENE parameter empty for the default display.
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.5&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=0GJ:L-ALPHA-GLUTAMYL-N-{(1S)-4-{[AMINO(IMINIO)METHYL]AMINO}-1-[(1S)-2-CHLORO-1-HYDROXYETHYL]BUTYL}GLYCINAMIDE'>0GJ</scene></td></tr>
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{{STRUCTURE_1bqy| PDB=1bqy | SCENE= }}
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1bqy FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1bqy OCA], [https://pdbe.org/1bqy PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1bqy RCSB], [https://www.ebi.ac.uk/pdbsum/1bqy PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1bqy ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/VSPPA_TRIST VSPPA_TRIST] Snake venom serine protease that activates plasminogen.<ref>PMID:7730329</ref>
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/bq/1bqy_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1bqy ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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BACKGROUND: Trimeresurus stejnejeri venom plasminogen activator (TSV-PA) is a snake venom serine proteinase that specifically activates plasminogen. Snake venom serine proteinases form a subfamily of trypsin-like proteinases that are characterised by a high substrate specificity and resistance to inhibition. Many of these venom enzymes specifically interfere with haemostatic mechanisms and display a long circulating half-life. For these reasons several of them have commercial applications and are potentially attractive pharmacological tools. RESULTS: The crystal structure of TSV-PA has been determined to 2.5 A resolution and refined to an R factor of 17.8 (R free, 24.4). The enzyme, showing the overall polypeptide fold of trypsin-like serine proteinases, displays unique structural elements such as the presence of a phenylalanine at position 193, a C-terminal tail clamped via a disulphide bridge to the 99-loop, and a structurally conserved Asp97 residue. The presence of a cis proline at position 218 is in agreement with evolutionary relationships to glandular kallikrein. CONCLUSIONS: We postulate that Phe 193 accounts for the high substrate specificity of TSV-PA and renders it incapable of forming a stable complex with bovine pancreatic trypsin inhibitor and other extended substrates and inhibitors. Mutational studies previously showed that Asp97 is crucial for the plasminogenolytic activity of TSV-PA, here we identify the conservation of Asp97 in both types of mammalian plasminogen activator - tissue-type (tPA) and urokinase-type (uPA). It seems likely that Asp97 of tPA and uPA will have a similar role in plasminogen recognition. The C-terminal extension of TSV-PA is conserved among snake venom serine proteinases, although its function is unknown. The three-dimensional structure presented here is the first of a snake venom serine proteinase and provides an excellent template for modelling other homologous family members.
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===PLASMINOGEN ACTIVATOR (TSV-PA) FROM SNAKE VENOM===
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The crystal structure of the novel snake venom plasminogen activator TSV-PA: a prototype structure for snake venom serine proteinases.,Parry MA, Jacob U, Huber R, Wisner A, Bon C, Bode W Structure. 1998 Sep 15;6(9):1195-206. PMID:9753698<ref>PMID:9753698</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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<div class="pdbe-citations 1bqy" style="background-color:#fffaf0;"></div>
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==See Also==
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The line below this paragraph, {{ABSTRACT_PUBMED_9753698}}, adds the Publication Abstract to the page
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*[[Plasminogen activator|Plasminogen activator]]
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(as it appears on PubMed at http://www.pubmed.gov), where 9753698 is the PubMed ID number.
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== References ==
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<references/>
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{{ABSTRACT_PUBMED_9753698}}
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__TOC__
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</StructureSection>
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==About this Structure==
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[[Category: Large Structures]]
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1BQY is a 4 chains structure of sequences from [http://en.wikipedia.org/wiki/Viridovipera_stejnegeri Viridovipera stejnegeri]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1BQY OCA].
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[[Category: Trimeresurus stejnegeri]]
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[[Category: Bode W]]
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==Reference==
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[[Category: Parry MAA]]
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<ref group="xtra">PMID:9753698</ref><references group="xtra"/>
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[[Category: Viridovipera stejnegeri]]
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[[Category: Bode, W.]]
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[[Category: Parry, M A.A.]]
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[[Category: Blood clotting]]
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[[Category: Fibrinolysis]]
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[[Category: Plasminogen activator]]
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[[Category: Serine proteinase]]
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[[Category: Snake venom]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Feb 16 17:23:43 2009''
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Current revision

Plasminogen activator (TSV-PA) from snake venom

PDB ID 1bqy

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