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2dpk
From Proteopedia
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| - | {{Seed}} | ||
| - | [[Image:2dpk.png|left|200px]] | ||
| - | < | + | ==The Crystal Structure of the Primary Ca2+ Sensor of the Na+/Ca2+ Exchanger== |
| - | + | <StructureSection load='2dpk' size='340' side='right'caption='[[2dpk]], [[Resolution|resolution]] 2.50Å' scene=''> | |
| - | + | == Structural highlights == | |
| - | + | <table><tr><td colspan='2'>[[2dpk]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Canlf Canlf]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2DPK OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2DPK FirstGlance]. <br> | |
| - | + | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=EPE:4-(2-HYDROXYETHYL)-1-PIPERAZINE+ETHANESULFONIC+ACID'>EPE</scene>, <scene name='pdbligand=GAI:GUANIDINE'>GAI</scene></td></tr> | |
| - | - | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2dpk FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2dpk OCA], [https://pdbe.org/2dpk PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2dpk RCSB], [https://www.ebi.ac.uk/pdbsum/2dpk PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2dpk ProSAT]</span></td></tr> |
| - | + | </table> | |
| + | == Function == | ||
| + | [[https://www.uniprot.org/uniprot/NAC1_CANFA NAC1_CANFA]] Rapidly transports Ca(2+) during excitation-contraction coupling. Ca(2+) is extruded from the cell during relaxation so as to prevent overloading of intracellular stores. | ||
| + | == Evolutionary Conservation == | ||
| + | [[Image:Consurf_key_small.gif|200px|right]] | ||
| + | Check<jmol> | ||
| + | <jmolCheckbox> | ||
| + | <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/dp/2dpk_consurf.spt"</scriptWhenChecked> | ||
| + | <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked> | ||
| + | <text>to colour the structure by Evolutionary Conservation</text> | ||
| + | </jmolCheckbox> | ||
| + | </jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2dpk ConSurf]. | ||
| + | <div style="clear:both"></div> | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | The Na+/Ca2+ exchanger is a plasma membrane protein that regulates intracellular Ca2+ levels in cardiac myocytes. Transport activity is governed by Ca2+, and the primary Ca2+ sensor (CBD1) is located in a large cytoplasmic loop connecting two transmembrane helices. The binding of Ca2+ to the CBD1 sensory domain results in conformational changes that stimulate the exchanger to extrude Ca2+. Here, we present a crystal structure of CBD1 at 2.5A resolution, which reveals a novel Ca2+ binding site consisting of four Ca2+ ions arranged in a tight planar cluster. This intricate coordination pattern for a Ca2+ binding cluster is indicative of a highly sensitive Ca2+ sensor and may represent a general platform for Ca2+ sensing. | ||
| - | + | The crystal structure of the primary Ca2+ sensor of the Na+/Ca2+ exchanger reveals a novel Ca2+ binding motif.,Nicoll DA, Sawaya MR, Kwon S, Cascio D, Philipson KD, Abramson J J Biol Chem. 2006 Aug 4;281(31):21577-81. Epub 2006 Jun 14. PMID:16774926<ref>PMID:16774926</ref> | |
| - | + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |
| - | + | </div> | |
| - | + | <div class="pdbe-citations 2dpk" style="background-color:#fffaf0;"></div> | |
| - | + | == References == | |
| - | + | <references/> | |
| - | + | __TOC__ | |
| - | + | </StructureSection> | |
| - | == | + | [[Category: Canlf]] |
| - | + | [[Category: Large Structures]] | |
| - | + | [[Category: Abramson, J]] | |
| - | == | + | [[Category: Sawaya, M]] |
| - | < | + | |
| - | [[Category: | + | |
| - | [[Category: Abramson, J | + | |
| - | [[Category: Sawaya, M | + | |
[[Category: Beta-bulge]] | [[Category: Beta-bulge]] | ||
[[Category: Beta-sandwich]] | [[Category: Beta-sandwich]] | ||
[[Category: Calcium sensory domain]] | [[Category: Calcium sensory domain]] | ||
[[Category: Cis-proline]] | [[Category: Cis-proline]] | ||
| - | + | [[Category: Metal transport]] | |
| - | + | ||
Current revision
The Crystal Structure of the Primary Ca2+ Sensor of the Na+/Ca2+ Exchanger
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