2i4c

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{{Seed}}
 
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[[Image:2i4c.png|left|200px]]
 
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==Crystal structure of Bicarbonate Transport Protein CmpA from Synechocystis sp. PCC 6803 in complex with bicarbonate and calcium==
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The line below this paragraph, containing "STRUCTURE_2i4c", creates the "Structure Box" on the page.
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<StructureSection load='2i4c' size='340' side='right'caption='[[2i4c]], [[Resolution|resolution]] 1.70&Aring;' scene=''>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>[[2i4c]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Synechocystis_sp._PCC_6803 Synechocystis sp. PCC 6803]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2I4C OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2I4C FirstGlance]. <br>
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or leave the SCENE parameter empty for the default display.
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.7&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=BCT:BICARBONATE+ION'>BCT</scene>, <scene name='pdbligand=CA:CALCIUM+ION'>CA</scene></td></tr>
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{{STRUCTURE_2i4c| PDB=2i4c | SCENE= }}
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2i4c FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2i4c OCA], [https://pdbe.org/2i4c PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2i4c RCSB], [https://www.ebi.ac.uk/pdbsum/2i4c PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2i4c ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/CMPA_SYNY3 CMPA_SYNY3] Part of the ABC transporter complex CmpABCD involved in bicarbonate transport. Binds bicarbonate with high affinity (By similarity).
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/i4/2i4c_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2i4c ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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Cyanobacteria, blue-green algae, are the most abundant autotrophs in aquatic environments and form the base of the food chain by fixing carbon and nitrogen into cellular biomass. To compensate for the low selectivity of Rubisco for CO2 over O2, cyanobacteria have developed highly efficient CO2-concentrating machinery of which the ABC transport system CmpABCD from Synechocystis PCC 6803 is one component. Here, we have described the structure of the bicarbonate-binding protein CmpA in the absence and presence of bicarbonate and carbonic acid. CmpA is highly homologous to the nitrate transport protein NrtA. CmpA binds carbonic acid at the entrance to the ligand-binding pocket, whereas bicarbonate binds in nearly an identical location compared with nitrate binding to NrtA. Unexpectedly, bicarbonate binding is accompanied by a metal ion, identified as Ca2+ via inductively coupled plasma optical emission spectrometry. The binding of bicarbonate and metal appears to be highly cooperative and suggests that CmpA may co-transport bicarbonate and calcium or that calcium acts a cofactor in bicarbonate transport.
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===Crystal structure of Bicarbonate Transport Protein CmpA from Synechocystis sp. PCC 6803 in complex with bicarbonate and calcium===
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The structure of a cyanobacterial bicarbonate transport protein, CmpA.,Koropatkin NM, Koppenaal DW, Pakrasi HB, Smith TJ J Biol Chem. 2007 Jan 26;282(4):2606-14. Epub 2006 Nov 22. PMID:17121816<ref>PMID:17121816</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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The line below this paragraph, {{ABSTRACT_PUBMED_17121816}}, adds the Publication Abstract to the page
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<div class="pdbe-citations 2i4c" style="background-color:#fffaf0;"></div>
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(as it appears on PubMed at http://www.pubmed.gov), where 17121816 is the PubMed ID number.
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== References ==
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<references/>
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{{ABSTRACT_PUBMED_17121816}}
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__TOC__
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</StructureSection>
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==About this Structure==
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[[Category: Large Structures]]
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2I4C is a 1 chain structure of sequence from [http://en.wikipedia.org/wiki/Synechocystis_sp. Synechocystis sp.]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2I4C OCA].
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[[Category: Synechocystis sp. PCC 6803]]
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[[Category: Koropatkin NM]]
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==Reference==
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[[Category: Pakrasi HB]]
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<ref group="xtra">PMID:17121816</ref><references group="xtra"/>
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[[Category: Smith TJ]]
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[[Category: Synechocystis sp.]]
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[[Category: Koropatkin, N M.]]
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[[Category: Pakrasi, H B.]]
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[[Category: Smith, T J.]]
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[[Category: Abc transporter]]
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[[Category: Alpha-beta protein]]
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[[Category: Bicarbonate]]
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[[Category: C-clamp]]
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[[Category: Calcium]]
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[[Category: Periplasmic solute-binding protein]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Feb 16 17:36:10 2009''
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Current revision

Crystal structure of Bicarbonate Transport Protein CmpA from Synechocystis sp. PCC 6803 in complex with bicarbonate and calcium

PDB ID 2i4c

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