1y2y

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{{Seed}}
 
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[[Image:1y2y.png|left|200px]]
 
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==Structural Characterization of Nop10p using Nuclear Magnetic Resonance Spectroscopy==
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The line below this paragraph, containing "STRUCTURE_1y2y", creates the "Structure Box" on the page.
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<StructureSection load='1y2y' size='340' side='right'caption='[[1y2y]]' scene=''>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>[[1y2y]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1Y2Y OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1Y2Y FirstGlance]. <br>
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or leave the SCENE parameter empty for the default display.
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1y2y FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1y2y OCA], [https://pdbe.org/1y2y PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1y2y RCSB], [https://www.ebi.ac.uk/pdbsum/1y2y PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1y2y ProSAT]</span></td></tr>
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{{STRUCTURE_1y2y| PDB=1y2y | SCENE= }}
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/NOP10_YEAST NOP10_YEAST] Required for ribosome biogenesis. Part of a complex which catalyzes pseudouridylation of rRNA. This involves the isomerization of uridine such that the ribose is subsequently attached to C5, instead of the normal N1. Pseudouridine ("psi") residues may serve to stabilize the conformation of rRNAs. Essential for growth.<ref>PMID:9843512</ref>
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/y2/1y2y_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1y2y ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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The H/ACA small nucleolar ribonucleoprotein (snoRNP) complexes guide the modification of uridine to pseudouridine at conserved sites in rRNA. The H/ACA snoRNPs each comprise a target-site-specific snoRNA and four core proteins, Nop10p, Nhp2p, Gar1p, and the pseudouridine synthase, Cbf5p, in yeast. The secondary structure of the H/ACA snoRNAs includes two hairpins that each contain a large internal loop (the pseudouridylation pocket), one or both of which are partially complementary to the target RNA(s). We have determined the solution structure of an RNA hairpin derived from the human U65 box H/ACA snoRNA including the pseudouridylation pocket and adjacent stems, providing the first three-dimensional structural information on these H/ACA snoRNAs. We have also determined the structure of Nop10p and investigated its interaction with RNA using NMR spectroscopy. Nop10p contains a structurally well-defined N-terminal region composed of a beta-hairpin, and the rest of the protein lacks a globular structure. Chemical shift mapping of the interaction of RNA constructs of U65 box H/ACA 3' hairpin with Nop10p shows that the beta-hairpin binds weakly but specifically to RNA. The unstructured region of Nop10p likely interacts with Cbf5p.
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===Structural Characterization of Nop10p using Nuclear Magnetic Resonance Spectroscopy===
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Structural study of the H/ACA snoRNP components Nop10p and the 3' hairpin of U65 snoRNA.,Khanna M, Wu H, Johansson C, Caizergues-Ferrer M, Feigon J RNA. 2006 Jan;12(1):40-52. PMID:16373493<ref>PMID:16373493</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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<div class="pdbe-citations 1y2y" style="background-color:#fffaf0;"></div>
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==See Also==
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The line below this paragraph, {{ABSTRACT_PUBMED_16373493}}, adds the Publication Abstract to the page
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*[[Ribosome biogenesis protein 3D structures|Ribosome biogenesis protein 3D structures]]
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(as it appears on PubMed at http://www.pubmed.gov), where 16373493 is the PubMed ID number.
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== References ==
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<references/>
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{{ABSTRACT_PUBMED_16373493}}
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__TOC__
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</StructureSection>
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==About this Structure==
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[[Category: Large Structures]]
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1Y2Y is a 1 chain structure of sequence from [http://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1Y2Y OCA].
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==Reference==
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<ref group="xtra">PMID:16373493</ref><references group="xtra"/>
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[[Category: Saccharomyces cerevisiae]]
[[Category: Saccharomyces cerevisiae]]
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[[Category: Caizergues-Ferrer, M.]]
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[[Category: Caizergues-Ferrer M]]
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[[Category: Feigon, J.]]
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[[Category: Feigon J]]
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[[Category: Johansson, C.]]
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[[Category: Johansson C]]
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[[Category: Khanna, M.]]
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[[Category: Khanna M]]
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[[Category: Wu, H.]]
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[[Category: Wu H]]
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[[Category: Box h/aca snorna,snornp]]
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[[Category: Pseudouridine]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Feb 16 17:47:06 2009''
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Current revision

Structural Characterization of Nop10p using Nuclear Magnetic Resonance Spectroscopy

PDB ID 1y2y

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