1yrn

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(New page: 200px<br /><applet load="1yrn" size="450" color="white" frame="true" align="right" spinBox="true" caption="1yrn, resolution 2.500&Aring;" /> '''CRYSTAL STRUCTURE O...)
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[[Image:1yrn.jpg|left|200px]]<br /><applet load="1yrn" size="450" color="white" frame="true" align="right" spinBox="true"
 
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caption="1yrn, resolution 2.500&Aring;" />
 
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'''CRYSTAL STRUCTURE OF THE MATA1/MATALPHA2 HOMEODOMAIN HETERODIMER BOUND TO DNA'''<br />
 
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==Overview==
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==CRYSTAL STRUCTURE OF THE MATA1/MATALPHA2 HOMEODOMAIN HETERODIMER BOUND TO DNA==
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The Saccharomyces cerevisiae MATa1 and MAT alpha 2 homeodomain proteins, which play a role in determining yeast cell type, form a heterodimer that, binds DNA and represses transcription in a cell type-specific manner., Whereas the alpha 2 and a1 proteins on their own have only modest affinity, for DNA, the a1/alpha 2 heterodimer binds DNA with high specificity and, affinity. The three-dimensional crystal structure of the a1/alpha 2, homeodomain heterodimer bound to DNA was determined at a resolution of 2.5, A. The a1 and alpha 2 homeodomains bind in a head-to-tail orientation, with heterodimer contacts mediated by a 16-residue tail located, carboxyl-terminal to the alpha 2 homeodomain. This tail becomes ordered in, the presence of a1, part of it forming a short amphipathic helix that, packs against the a1 homeodomain between helices 1 and 2. A pronounced 60, degree bend is induced in the DNA, which makes possible protein-protein, and protein-DNA contacts that could not take place in a straight DNA, fragment. Complex formation mediated by flexible protein-recognition, peptides attached to stably folded DNA binding domains may prove to be a, general feature of the architecture of other classes of eukaryotic, transcriptional regulators.
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<StructureSection load='1yrn' size='340' side='right'caption='[[1yrn]], [[Resolution|resolution]] 2.50&Aring;' scene=''>
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== Structural highlights ==
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==About this Structure==
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<table><tr><td colspan='2'>[[1yrn]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1YRN OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1YRN FirstGlance]. <br>
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1YRN is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1YRN OCA].
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.5&#8491;</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1yrn FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1yrn OCA], [https://pdbe.org/1yrn PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1yrn RCSB], [https://www.ebi.ac.uk/pdbsum/1yrn PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1yrn ProSAT]</span></td></tr>
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==Reference==
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</table>
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Crystal structure of the MATa1/MAT alpha 2 homeodomain heterodimer bound to DNA., Li T, Stark MR, Johnson AD, Wolberger C, Science. 1995 Oct 13;270(5234):262-9. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=7569974 7569974]
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== Function ==
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[[Category: Protein complex]]
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[https://www.uniprot.org/uniprot/MATA1_YEASX MATA1_YEASX] Mating type proteins are sequence specific DNA-binding proteins that act as master switches in yeast differentiation by controlling gene expression in a cell type-specific fashion. Transcriptional corepressor that, in a/alpha diploid cells, binds cooperatively with the ALPHA2 protein to a 21-bp DNA sequence termed the haploid-specific gene (hsg) operator, to repress transcription of haploid-specific genes and of MATALPHA1.<ref>PMID:8664541</ref>
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/yr/1yrn_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1yrn ConSurf].
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<div style="clear:both"></div>
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== References ==
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<references/>
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__TOC__
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</StructureSection>
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[[Category: Large Structures]]
[[Category: Saccharomyces cerevisiae]]
[[Category: Saccharomyces cerevisiae]]
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[[Category: Johnson, A.D.]]
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[[Category: Johnson AD]]
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[[Category: Li, T.]]
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[[Category: Li T]]
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[[Category: Stark, M.R.]]
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[[Category: Stark MR]]
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[[Category: Wolberger, C.]]
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[[Category: Wolberger C]]
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[[Category: complex]]
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[[Category: dna]]
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[[Category: dna-binding protein]]
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''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Wed Nov 21 07:00:19 2007''
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Current revision

CRYSTAL STRUCTURE OF THE MATA1/MATALPHA2 HOMEODOMAIN HETERODIMER BOUND TO DNA

PDB ID 1yrn

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