1p9e

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{{Seed}}
 
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[[Image:1p9e.png|left|200px]]
 
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==Crystal Structure Analysis of Methyl Parathion Hydrolase from Pseudomonas sp WBC-3==
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The line below this paragraph, containing "STRUCTURE_1p9e", creates the "Structure Box" on the page.
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<StructureSection load='1p9e' size='340' side='right'caption='[[1p9e]], [[Resolution|resolution]] 2.40&Aring;' scene=''>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>[[1p9e]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Pseudomonas_sp._WBC-3 Pseudomonas sp. WBC-3]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1P9E OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1P9E FirstGlance]. <br>
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or leave the SCENE parameter empty for the default display.
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.4&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CD:CADMIUM+ION'>CD</scene>, <scene name='pdbligand=K:POTASSIUM+ION'>K</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
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{{STRUCTURE_1p9e| PDB=1p9e | SCENE= }}
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1p9e FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1p9e OCA], [https://pdbe.org/1p9e PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1p9e RCSB], [https://www.ebi.ac.uk/pdbsum/1p9e PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1p9e ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/Q841S6_PSEWB Q841S6_PSEWB]
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/p9/1p9e_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview03.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1p9e ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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Methyl parathion hydrolase (MPH, E.C.3.1.8.1), isolated from the soil-dwelling bacterium Pseudomonas sp. WBC-3, is a Zn(II)-containing enzyme that catalyzes the degradation of the organophosphate pesticide methyl parathion. We have determined the structure of MPH from Pseudomonas sp. WBC-3 to 2.4 angstroms resolution. The enzyme is dimeric and each subunit contains a mixed hybrid binuclear zinc center, in which one of the zinc ions is replaced by cadmium. In both subunits, the more solvent-exposed beta-metal ion is substituted for Cd2+ due to high cadmium concentration in the crystallization condition. Both ions are surrounded by ligands in an octahedral arrangement. The ions are separated by 3.5 angstroms and are coordinated by the amino acid residues His147, His149, Asp151, His152, His234 and His302 and a water molecule. Asp255 and a water molecule serve to bridge the zinc ions together. MPH is homologous with other metallo-beta-lactamases but does not show any similarity to phosphotriesterase that can also catalyze the degradation of methyl parathion with lower rate, despite the lack of sequence homology. Trp179, Phe196 and Phe119 form an aromatic cluster at the entrance of the catalytic center. Replacement of these three amino acids by alanine resulted in a significant increase of K(m) and loss of catalytic activity, indicating that the aromatic cluster has an important role to facilitate affinity of enzyme to the methyl parathion substrates.
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===Crystal Structure Analysis of Methyl Parathion Hydrolase from Pseudomonas sp WBC-3===
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Crystal structure of methyl parathion hydrolase from Pseudomonas sp. WBC-3.,Dong YJ, Bartlam M, Sun L, Zhou YF, Zhang ZP, Zhang CG, Rao Z, Zhang XE J Mol Biol. 2005 Oct 28;353(3):655-63. doi: 10.1016/j.jmb.2005.08.057. Epub 2005 , Sep 8. PMID:16181636<ref>PMID:16181636</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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==About this Structure==
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</div>
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1P9E is a 2 chains structure of sequences from [http://en.wikipedia.org/wiki/Pseudomonas_sp. Pseudomonas sp.]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1P9E OCA].
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<div class="pdbe-citations 1p9e" style="background-color:#fffaf0;"></div>
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[[Category: Aryldialkylphosphatase]]
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== References ==
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[[Category: Pseudomonas sp.]]
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<references/>
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[[Category: Bartlam, M.]]
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__TOC__
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[[Category: Dong, Y.]]
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</StructureSection>
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[[Category: Rao, Z.]]
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[[Category: Large Structures]]
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[[Category: Sun, L.]]
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[[Category: Pseudomonas sp. WBC-3]]
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[[Category: Zhang, X.]]
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[[Category: Bartlam M]]
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[[Category: Zn containing]]
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[[Category: Dong Y]]
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[[Category: Rao Z]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Feb 16 17:51:40 2009''
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[[Category: Sun L]]
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[[Category: Zhang X]]

Current revision

Crystal Structure Analysis of Methyl Parathion Hydrolase from Pseudomonas sp WBC-3

PDB ID 1p9e

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