1ysb

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(New page: 200px<br /><applet load="1ysb" size="450" color="white" frame="true" align="right" spinBox="true" caption="1ysb, resolution 1.70&Aring;" /> '''Yeast Cytosine Deami...)
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[[Image:1ysb.gif|left|200px]]<br /><applet load="1ysb" size="450" color="white" frame="true" align="right" spinBox="true"
 
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caption="1ysb, resolution 1.70&Aring;" />
 
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'''Yeast Cytosine Deaminase Triple Mutant'''<br />
 
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==Overview==
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==Yeast Cytosine Deaminase Triple Mutant==
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Thermostabilizing an enzyme while maintaining its activity for industrial, or biomedical applications can be difficult with traditional selection, methods. We describe a rapid computational approach that identified three, mutations within a model enzyme that produced a 10 degrees C increase in, apparent melting temperature T(m) and a 30-fold increase in half-life at, 50 degrees C, with no reduction in catalytic efficiency. The effects of, the mutations were synergistic, giving an increase in excess of the sum of, their individual effects. The redesigned enzyme induced an increased, temperature-dependent bacterial growth rate under conditions that required, its activity, thereby coupling molecular and metabolic engineering.
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<StructureSection load='1ysb' size='340' side='right'caption='[[1ysb]], [[Resolution|resolution]] 1.70&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[1ysb]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1YSB OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1YSB FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.7&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1ysb FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1ysb OCA], [https://pdbe.org/1ysb PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1ysb RCSB], [https://www.ebi.ac.uk/pdbsum/1ysb PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1ysb ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/FCY1_YEAST FCY1_YEAST] Converts cytosine to uracil or 5-methylcytosine to thymine by deaminating carbon number 4.
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ys/1ysb_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1ysb ConSurf].
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<div style="clear:both"></div>
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==About this Structure==
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==See Also==
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1YSB is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae] with ZN and CA as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/Cytosine_deaminase Cytosine deaminase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.5.4.1 3.5.4.1] Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1YSB OCA].
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*[[Deaminase 3D structures|Deaminase 3D structures]]
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__TOC__
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==Reference==
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</StructureSection>
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Computational thermostabilization of an enzyme., Korkegian A, Black ME, Baker D, Stoddard BL, Science. 2005 May 6;308(5723):857-60. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=15879217 15879217]
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[[Category: Large Structures]]
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[[Category: Cytosine deaminase]]
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[[Category: Saccharomyces cerevisiae]]
[[Category: Saccharomyces cerevisiae]]
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[[Category: Single protein]]
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[[Category: Baker D]]
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[[Category: Baker, D.]]
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[[Category: Black ME]]
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[[Category: Black, M.E.]]
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[[Category: Korkegian A]]
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[[Category: Korkegian, A.]]
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[[Category: Stoddard BL]]
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[[Category: Stoddard, B.L.]]
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[[Category: CA]]
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[[Category: ZN]]
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[[Category: hydrolase]]
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''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Wed Nov 21 07:01:10 2007''
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Current revision

Yeast Cytosine Deaminase Triple Mutant

PDB ID 1ysb

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