2c7g

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{{Seed}}
 
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[[Image:2c7g.png|left|200px]]
 
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==FprA from Mycobacterium tuberculosis: His57Gln mutant==
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The line below this paragraph, containing "STRUCTURE_2c7g", creates the "Structure Box" on the page.
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<StructureSection load='2c7g' size='340' side='right'caption='[[2c7g]], [[Resolution|resolution]] 1.80&Aring;' scene=''>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>[[2c7g]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Mycobacterium_tuberculosis Mycobacterium tuberculosis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2C7G OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2C7G FirstGlance]. <br>
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or leave the SCENE parameter empty for the default display.
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.8&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=FAD:FLAVIN-ADENINE+DINUCLEOTIDE'>FAD</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene>, <scene name='pdbligand=ODP:4-OXO-NICOTINAMIDE-ADENINE+DINUCLEOTIDE+PHOSPHATE'>ODP</scene></td></tr>
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{{STRUCTURE_2c7g| PDB=2c7g | SCENE= }}
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2c7g FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2c7g OCA], [https://pdbe.org/2c7g PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2c7g RCSB], [https://www.ebi.ac.uk/pdbsum/2c7g PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2c7g ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/FPRA_MYCTU FPRA_MYCTU] May serve as electron transfer protein and supply electrons to P450 systems.
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/c7/2c7g_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2c7g ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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Mycobacterium tuberculosis FprA is a NADPH-ferredoxin reductase, functionally and structurally similar to the mammalian adrenodoxin reductase. It is presumably involved in supplying electrons to one or more of the pathogen's cytochrome P450s through reduced ferredoxins. It has been proposed on the basis of crystallographic data (Bossi, R. T., et al. (2002) Biochemistry 41, 8807-8818) that the highly conserved His57 and Glu214 whose side chains are H-bonded are involved in catalysis. Both residues were individually changed to nonionizable amino acyl residues through site-directed mutagenesis. Steady-state kinetics showed that the role of Glu214 in catalysis is negligible. On the contrary, the substitutions of His57 markedly impaired the catalytic efficiency of FprA for ferredoxin in the physiological reaction. Furthemore, they decreased the k(cat)/K(m) value for NADPH in the ferricyanide reduction. Rapid-reaction (stopped-flow) kinetic analysis of the isolated reductive half-reaction of wild-type and His57Gln forms of FprA with NADPH and NADH allowed a detailed description of the mechanism of enzyme-bound FAD reduction, with the identification of the intermediates involved. The His57Gln mutation caused a 6-fold decrease in the rate of hydride transfer from either NADPH or NADH to the enzyme-bound FAD cofactor. The 3D structure of FprA-H57Q, obtained at 1.8 A resolution, explains the inefficient hydride transfer of the mutant in terms of a suboptimal geometry of the nicotinamide-isoalloxazine interaction in the active site. These data demonstrate the role of His57 in the correct binding of NADPH to FprA for the subsequent steps of the catalytic cycle to proceed at a high rate.
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===FPRA FROM MYCOBACTERIUM TUBERCULOSIS: HIS57GLN MUTANT===
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Role of the His57-Glu214 ionic couple located in the active site of Mycobacterium tuberculosis FprA.,Pennati A, Razeto A, de Rosa M, Pandini V, Vanoni MA, Mattevi A, Coda A, Aliverti A, Zanetti G Biochemistry. 2006 Jul 25;45(29):8712-20. PMID:16846214<ref>PMID:16846214</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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The line below this paragraph, {{ABSTRACT_PUBMED_16846214}}, adds the Publication Abstract to the page
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<div class="pdbe-citations 2c7g" style="background-color:#fffaf0;"></div>
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(as it appears on PubMed at http://www.pubmed.gov), where 16846214 is the PubMed ID number.
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== References ==
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<references/>
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{{ABSTRACT_PUBMED_16846214}}
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__TOC__
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</StructureSection>
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==About this Structure==
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[[Category: Large Structures]]
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2C7G is a 1 chain structure of sequence from [http://en.wikipedia.org/wiki/Mycobacterium_tuberculosis Mycobacterium tuberculosis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2C7G OCA].
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==Reference==
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<ref group="xtra">PMID:16846214</ref><references group="xtra"/>
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[[Category: Mycobacterium tuberculosis]]
[[Category: Mycobacterium tuberculosis]]
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[[Category: Aliverti, A.]]
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[[Category: Aliverti A]]
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[[Category: Coda, A.]]
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[[Category: Coda A]]
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[[Category: Mattevi, A.]]
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[[Category: De Rosa M]]
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[[Category: Pandini, V.]]
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[[Category: Mattevi A]]
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[[Category: Pennati, A.]]
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[[Category: Pandini V]]
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[[Category: Razeto, A.]]
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[[Category: Pennati A]]
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[[Category: Rosa, M De.]]
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[[Category: Razeto A]]
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[[Category: Vanoni, M A.]]
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[[Category: Vanoni MA]]
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[[Category: Zanetti, G.]]
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[[Category: Zanetti G]]
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[[Category: Fad]]
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[[Category: Flavoprotein]]
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[[Category: Nadp]]
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[[Category: Nap+ derivative]]
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[[Category: Oxidoreductase]]
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[[Category: Tuberculosis]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Feb 16 18:09:28 2009''
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Current revision

FprA from Mycobacterium tuberculosis: His57Gln mutant

PDB ID 2c7g

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