1yv0

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(New page: 200px<br /><applet load="1yv0" size="450" color="white" frame="true" align="right" spinBox="true" caption="1yv0, resolution 7.0&Aring;" /> '''Crystal structure of ...)
Current revision (09:00, 14 February 2024) (edit) (undo)
 
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[[Image:1yv0.jpg|left|200px]]<br /><applet load="1yv0" size="450" color="white" frame="true" align="right" spinBox="true"
 
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caption="1yv0, resolution 7.0&Aring;" />
 
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'''Crystal structure of skeletal muscle troponin in the Ca2+-free state'''<br />
 
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==Overview==
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==Crystal structure of skeletal muscle troponin in the Ca2+-free state==
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Troponin senses Ca2+ to regulate contraction in striated muscle., Structures of skeletal muscle troponin composed of TnC (the sensor), TnI, (the regulator), and TnT (the link to the muscle thin filament) have been, determined. The structure of troponin in the Ca(2+)-activated state, features a nearly twofold symmetrical assembly of TnI and TnT subunits, penetrated asymmetrically by the dumbbell-shaped TnC subunit. Ca ions are, thought to regulate contraction by controlling the presentation to and, withdrawal of the TnI inhibitory segment from the thin filament. Here, we, show that the rigid central helix of the sensor binds the inhibitory, segment of TnI in the Ca(2+)-activated state. Comparison of crystal, structures of troponin in the Ca(2+)-activated state at 3.0 angstroms, resolution and in the Ca(2+)-free state at 7.0 angstroms resolution shows, that the long framework helices of TnI and TnT, presumed to be a, Ca(2+)-independent structural domain of troponin are unchanged. Loss of Ca, ions causes the rigid central helix of the sensor to collapse and to, release the inhibitory segment of TnI. The inhibitory segment of TnI, changes conformation from an extended loop in the presence of Ca2+ to a, short alpha-helix in its absence. We also show that Anapoe, a detergent, molecule, increases the contractile force of muscle fibers and binds, specifically, together with the TnI switch helix, in a hydrophobic pocket, of TnC upon activation by Ca ions.
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<StructureSection load='1yv0' size='340' side='right'caption='[[1yv0]], [[Resolution|resolution]] 7.00&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[1yv0]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Gallus_gallus Gallus gallus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1YV0 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1YV0 FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 7&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1yv0 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1yv0 OCA], [https://pdbe.org/1yv0 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1yv0 RCSB], [https://www.ebi.ac.uk/pdbsum/1yv0 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1yv0 ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/TNNT3_CHICK TNNT3_CHICK] Troponin T is the tropomyosin-binding subunit of troponin, the thin filament regulatory complex which confers calcium-sensitivity to striated muscle actomyosin ATPase activity.
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/yv/1yv0_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1yv0 ConSurf].
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<div style="clear:both"></div>
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==About this Structure==
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==See Also==
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1YV0 is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Gallus_gallus Gallus gallus] with MG as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1YV0 OCA].
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*[[Troponin|Troponin]]
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*[[Troponin 3D structures|Troponin 3D structures]]
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==Reference==
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__TOC__
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Ca(2+)-regulated structural changes in troponin., Vinogradova MV, Stone DB, Malanina GG, Karatzaferi C, Cooke R, Mendelson RA, Fletterick RJ, Proc Natl Acad Sci U S A. 2005 Apr 5;102(14):5038-43. Epub 2005 Mar 22. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=15784741 15784741]
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</StructureSection>
[[Category: Gallus gallus]]
[[Category: Gallus gallus]]
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[[Category: Protein complex]]
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[[Category: Large Structures]]
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[[Category: Cooke, R.]]
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[[Category: Cooke R]]
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[[Category: Fletterick, R.J.]]
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[[Category: Fletterick RJ]]
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[[Category: Karatzaferi, C.]]
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[[Category: Karatzaferi C]]
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[[Category: Malanina, G.G.]]
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[[Category: Malanina GG]]
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[[Category: Mendelson, R.A.]]
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[[Category: Mendelson RA]]
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[[Category: Stone, D.B.]]
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[[Category: Stone DB]]
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[[Category: Vinogradova, M.V.]]
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[[Category: Vinogradova MV]]
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[[Category: MG]]
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[[Category: actin binding]]
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[[Category: muscle]]
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[[Category: off state]]
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[[Category: thin filament]]
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[[Category: troponin]]
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''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Wed Nov 21 07:04:12 2007''
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Current revision

Crystal structure of skeletal muscle troponin in the Ca2+-free state

PDB ID 1yv0

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