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1yyp

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(New page: 200px<br /><applet load="1yyp" size="450" color="white" frame="true" align="right" spinBox="true" caption="1yyp, resolution 2.500&Aring;" /> '''Crystal structure o...)
Current revision (09:01, 14 February 2024) (edit) (undo)
 
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[[Image:1yyp.gif|left|200px]]<br /><applet load="1yyp" size="450" color="white" frame="true" align="right" spinBox="true"
 
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caption="1yyp, resolution 2.500&Aring;" />
 
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'''Crystal structure of cytomegalovirus UL44 bound to C-terminal peptide from CMV UL54'''<br />
 
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==Overview==
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==Crystal structure of cytomegalovirus UL44 bound to C-terminal peptide from CMV UL54==
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The human cytomegalovirus DNA polymerase is composed of a catalytic, subunit, UL54, and an accessory protein, UL44, which has a structural fold, similar to that of other processivity factors, including herpes simplex, virus UL42 and homotrimeric sliding clamps such as proliferating cell, nuclear antigen. Several specific residues in the C-terminal region of, UL54 and in the "connector loop" of UL44 are required for the association, of these proteins. Here, we describe the crystal structure of residues, 1-290 of UL44 in complex with a peptide from the extreme C terminus of, UL54, which explains this interaction at a molecular level. The UL54, peptide binds to structural elements similar to those used by UL42 and the, sliding clamps to associate with their respective binding partners., However, the details of the interaction differ from those of other, processivity factor-peptide complexes. Crucial residues include a, three-residue hydrophobic "plug" from the UL54 peptide and Ile(135) of, UL44, which forms a critical intramolecular hydrophobic anchor for, interactions between the connector loop and the peptide. As was the case, for the unliganded UL44 structure, the UL44-peptide complex forms a, head-to-head dimer that could potentially form a C-shaped clamp on DNA., However, the peptide-bound structure displays subtle differences in the, relative orientation of the two subdomains of the protein, resulting in a, more open clamp, which we predicted would affect its association with DNA., Indeed, filter binding assays revealed that peptide-bound UL44 binds DNA, with higher affinity. Thus, interaction with the catalytic subunit appears, to affect both the structure and function of UL44.
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<StructureSection load='1yyp' size='340' side='right'caption='[[1yyp]], [[Resolution|resolution]] 2.50&Aring;' scene=''>
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== Structural highlights ==
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==About this Structure==
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<table><tr><td colspan='2'>[[1yyp]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Human_betaherpesvirus_5 Human betaherpesvirus 5] and [https://en.wikipedia.org/wiki/Human_herpesvirus_5_strain_AD169 Human herpesvirus 5 strain AD169]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1YYP OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1YYP FirstGlance]. <br>
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1YYP is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Human_herpesvirus_5 Human herpesvirus 5] with SO4 and EDO as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/DNA-directed_DNA_polymerase DNA-directed DNA polymerase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.7.7 2.7.7.7] Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1YYP OCA].
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.5&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
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==Reference==
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1yyp FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1yyp OCA], [https://pdbe.org/1yyp PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1yyp RCSB], [https://www.ebi.ac.uk/pdbsum/1yyp PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1yyp ProSAT]</span></td></tr>
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Crystal structure of the cytomegalovirus DNA polymerase subunit UL44 in complex with the C terminus from the catalytic subunit. Differences in structure and function relative to unliganded UL44., Appleton BA, Brooks J, Loregian A, Filman DJ, Coen DM, Hogle JM, J Biol Chem. 2006 Feb 24;281(8):5224-32. Epub 2005 Dec 20. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=16371349 16371349]
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</table>
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[[Category: DNA-directed DNA polymerase]]
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== Function ==
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[[Category: Human herpesvirus 5]]
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[https://www.uniprot.org/uniprot/VPAP_HCMVA VPAP_HCMVA] Accessory subunit of the DNA polymerase that acts to increase the processivity of polymerization.<ref>PMID:20538862</ref>
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[[Category: Protein complex]]
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== Evolutionary Conservation ==
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[[Category: Appleton, B.A.]]
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[[Image:Consurf_key_small.gif|200px|right]]
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[[Category: Brooks, J.]]
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Check<jmol>
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[[Category: Coen, D.M.]]
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<jmolCheckbox>
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[[Category: Filman, D.J.]]
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/yy/1yyp_consurf.spt"</scriptWhenChecked>
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[[Category: Hogle, J.M.]]
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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[[Category: Loregian, A]]
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<text>to colour the structure by Evolutionary Conservation</text>
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[[Category: EDO]]
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</jmolCheckbox>
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[[Category: SO4]]
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1yyp ConSurf].
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[[Category: and homotrimeric sliding clamps)]]
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<div style="clear:both"></div>
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[[Category: pcna]]
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== References ==
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[[Category: processivity fold (same fold as hsv ul42]]
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<references/>
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__TOC__
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''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Wed Nov 21 07:08:17 2007''
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</StructureSection>
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[[Category: Human betaherpesvirus 5]]
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[[Category: Human herpesvirus 5 strain AD169]]
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[[Category: Large Structures]]
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[[Category: Appleton BA]]
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[[Category: Brooks J]]
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[[Category: Coen DM]]
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[[Category: Filman DJ]]
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[[Category: Hogle JM]]
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[[Category: Loregian A]]

Current revision

Crystal structure of cytomegalovirus UL44 bound to C-terminal peptide from CMV UL54

PDB ID 1yyp

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