1z0j

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(New page: 200px<br /><applet load="1z0j" size="450" color="white" frame="true" align="right" spinBox="true" caption="1z0j, resolution 1.32&Aring;" /> '''Structure of GTP-Bou...)
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[[Image:1z0j.gif|left|200px]]<br /><applet load="1z0j" size="450" color="white" frame="true" align="right" spinBox="true"
 
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caption="1z0j, resolution 1.32&Aring;" />
 
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'''Structure of GTP-Bound Rab22Q64L GTPase in complex with the minimal Rab binding domain of Rabenosyn-5'''<br />
 
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==Overview==
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==Structure of GTP-Bound Rab22Q64L GTPase in complex with the minimal Rab binding domain of Rabenosyn-5==
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Rab GTPases regulate all stages of membrane trafficking, including vesicle, budding, cargo sorting, transport, tethering and fusion. In the inactive, (GDP-bound) conformation, accessory factors facilitate the targeting of, Rab GTPases to intracellular compartments. After nucleotide exchange to, the active (GTP-bound) conformation, Rab GTPases interact with, functionally diverse effectors including lipid kinases, motor proteins and, tethering complexes. How effectors distinguish between homologous Rab, GTPases represents an unresolved problem with respect to the specificity, of vesicular trafficking. Using a structural proteomic approach, we have, determined the specificity and structural basis underlying the interaction, of the multivalent effector rabenosyn-5 with the Rab family. The results, demonstrate that even the structurally similar effector domains in, rabenosyn-5 can achieve highly selective recognition of distinct subsets, of Rab GTPases exclusively through interactions with the switch and, interswitch regions. The observed specificity is determined at a, family-wide level by structural diversity in the active conformation, which governs the spatial disposition of critical conserved recognition, determinants, and by a small number of both positive and negative sequence, determinants that allow further discrimination between Rab GTPases with, similar switch conformations.
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<StructureSection load='1z0j' size='340' side='right'caption='[[1z0j]], [[Resolution|resolution]] 1.32&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[1z0j]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] and [https://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1Z0J OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1Z0J FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.32&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=GTP:GUANOSINE-5-TRIPHOSPHATE'>GTP</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=TRS:2-AMINO-2-HYDROXYMETHYL-PROPANE-1,3-DIOL'>TRS</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1z0j FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1z0j OCA], [https://pdbe.org/1z0j PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1z0j RCSB], [https://www.ebi.ac.uk/pdbsum/1z0j PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1z0j ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/RB22A_MOUSE RB22A_MOUSE] Plays a role in endocytosis and intracellular protein transport. Mediates trafficking of TF from early endosomes to recycling endosomes. Required for NGF-mediated endocytosis of NTRK1, and subsequent neurite outgrowth (By similarity). Binds GTP and GDP and has low GTPase activity. Alternates between a GTP-bound active form and a GDP-bound inactive form.<ref>PMID:19759177</ref>
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/z0/1z0j_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1z0j ConSurf].
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<div style="clear:both"></div>
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==About this Structure==
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==See Also==
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1Z0J is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] and [http://en.wikipedia.org/wiki/Mus_musculus Mus musculus] with MG, GTP, TRS and GOL as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1Z0J OCA].
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*[[Ras-related protein Rab 3D structures|Ras-related protein Rab 3D structures]]
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== References ==
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==Reference==
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<references/>
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Structural basis of family-wide Rab GTPase recognition by rabenosyn-5., Eathiraj S, Pan X, Ritacco C, Lambright DG, Nature. 2005 Jul 21;436(7049):415-9. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=16034420 16034420]
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__TOC__
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</StructureSection>
[[Category: Homo sapiens]]
[[Category: Homo sapiens]]
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[[Category: Large Structures]]
[[Category: Mus musculus]]
[[Category: Mus musculus]]
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[[Category: Protein complex]]
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[[Category: Eathiraj S]]
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[[Category: Eathiraj, S.]]
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[[Category: Lambright DG]]
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[[Category: Lambright, D.G.]]
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[[Category: Pan X]]
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[[Category: Pan, X.]]
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[[Category: Ritacco C]]
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[[Category: Ritacco, C.]]
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[[Category: GOL]]
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[[Category: GTP]]
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[[Category: MG]]
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[[Category: TRS]]
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[[Category: endosomal trafficking]]
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[[Category: protein transport]]
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[[Category: rab effector]]
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[[Category: rab gtpase]]
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[[Category: rab22 gtpase]]
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[[Category: rabenosyn]]
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''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Wed Nov 21 07:10:13 2007''
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Current revision

Structure of GTP-Bound Rab22Q64L GTPase in complex with the minimal Rab binding domain of Rabenosyn-5

PDB ID 1z0j

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