1rl1

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{{Seed}}
 
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[[Image:1rl1.png|left|200px]]
 
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==Solution structure of human Sgt1 CS domain==
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The line below this paragraph, containing "STRUCTURE_1rl1", creates the "Structure Box" on the page.
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<StructureSection load='1rl1' size='340' side='right'caption='[[1rl1]]' scene=''>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>[[1rl1]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1RL1 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1RL1 FirstGlance]. <br>
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or leave the SCENE parameter empty for the default display.
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1rl1 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1rl1 OCA], [https://pdbe.org/1rl1 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1rl1 RCSB], [https://www.ebi.ac.uk/pdbsum/1rl1 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1rl1 ProSAT]</span></td></tr>
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{{STRUCTURE_1rl1| PDB=1rl1 | SCENE= }}
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/SGT1_HUMAN SGT1_HUMAN] May play a role in ubiquitination and subsequent proteasomal degradation of target proteins.
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/rl/1rl1_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1rl1 ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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Sgt1 has been identified as a subunit of both core kinetochore and SCF (Skp1-Cul1-F-box) ubiquitin ligase complexes and is also implicated in plant disease resistance. Sgt1 has two putative HSP90 binding domains, a tetratricopeptide repeat and a p23-like CHORD and Sgt1 (CS) domain. Using NMR spectroscopy, we show that only the CS domain of human Sgt1 physically interacts with HSP90. The tetratricopeptide repeat domain does not bind to either HSP90 or HSP70. Determination of the three-dimensional structure showed that the Sgt1-CS domain shares the same beta-sandwich fold as p23 but lacks the last highly conserved beta-strand in p23. Analysis of the structures of Sgt1-CS and p23 revealed a similar charge distribution on one of two opposing surfaces that suggests that it is the binding region for HSP90 in Sgt1. Although ATP is absolutely required for p23 binding to HSP90, Sgt1 binds to HSP90 also in the absence of the non-hydrolyzable analog ATPgammaS. Our findings suggest the CS domain is a binding module for HSP90 distinct from p23-like domains, which implies that Sgt1 and related proteins function in recruiting heat shock protein activities to multiprotein assemblies.
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===Solution structure of human Sgt1 CS domain===
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Human Sgt1 binds HSP90 through the CHORD-Sgt1 domain and not the tetratricopeptide repeat domain.,Lee YT, Jacob J, Michowski W, Nowotny M, Kuznicki J, Chazin WJ J Biol Chem. 2004 Apr 16;279(16):16511-7. Epub 2004 Feb 3. PMID:14761955<ref>PMID:14761955</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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The line below this paragraph, {{ABSTRACT_PUBMED_14761955}}, adds the Publication Abstract to the page
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<div class="pdbe-citations 1rl1" style="background-color:#fffaf0;"></div>
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(as it appears on PubMed at http://www.pubmed.gov), where 14761955 is the PubMed ID number.
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== References ==
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<references/>
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{{ABSTRACT_PUBMED_14761955}}
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__TOC__
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</StructureSection>
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==About this Structure==
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1RL1 is a 1 chain structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1RL1 OCA].
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==Reference==
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<ref group="xtra">PMID:14761955</ref><references group="xtra"/>
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[[Category: Homo sapiens]]
[[Category: Homo sapiens]]
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[[Category: Chazin, W J.]]
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[[Category: Large Structures]]
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[[Category: Jacob, J.]]
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[[Category: Chazin WJ]]
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[[Category: Kuznicki, J.]]
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[[Category: Jacob J]]
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[[Category: Lee, Y T.]]
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[[Category: Kuznicki J]]
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[[Category: Michowski, W.]]
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[[Category: Lee Y-T]]
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[[Category: Nowotny, M.]]
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[[Category: Michowski W]]
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[[Category: 7 beta strand]]
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[[Category: Nowotny M]]
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[[Category: Beta sandwich]]
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[[Category: Lacking last beta strand seen in p23]]
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[[Category: Similar to p23]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Feb 16 19:11:07 2009''
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Current revision

Solution structure of human Sgt1 CS domain

PDB ID 1rl1

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OCA

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