1z3e

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(New page: 200px<br /><applet load="1z3e" size="450" color="white" frame="true" align="right" spinBox="true" caption="1z3e, resolution 1.500&Aring;" /> '''Crystal Structure o...)
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[[Image:1z3e.gif|left|200px]]<br /><applet load="1z3e" size="450" color="white" frame="true" align="right" spinBox="true"
 
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caption="1z3e, resolution 1.500&Aring;" />
 
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'''Crystal Structure of Spx in Complex with the C-terminal Domain of the RNA Polymerase Alpha Subunit'''<br />
 
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==Overview==
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==Crystal Structure of Spx in Complex with the C-terminal Domain of the RNA Polymerase Alpha Subunit==
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Spx, a global transcription regulator in Bacillus subtilis, interacts with, the C-terminal domain of the alpha subunit (alphaCTD) of RNA polymerase to, control gene expression under conditions of disulfide stress, which is, sensed by disulfide bond formation between Spx residues C10 and C13. Here, we describe the crystal structure of the B. subtilis alphaCTD bound to, oxidized Spx. Analysis of the complex reveals interactions between three, regions of "anti-alpha" Spx and helix alpha1 and the "261" determinant of, alphaCTD. The former contact could disrupt the interaction between, alphaCTD and activator proteins or alter the DNA-bound conformation of, alphaCTD, thereby repressing activator-stimulated transcription. Binding, to the 261 determinant would prevent interaction between alphaCTD and, region 4 of sigma(A). Intriguingly, the Spx disulfide bond is far from the, alphaCTD-Spx interface, suggesting that Spx regulates transcription, allosterically or through the redox-dependent creation or destruction of, binding sites for additional components of the transcription machinery.
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<StructureSection load='1z3e' size='340' side='right'caption='[[1z3e]], [[Resolution|resolution]] 1.50&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[1z3e]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Bacillus_subtilis Bacillus subtilis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1Z3E OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1Z3E FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.5&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1z3e FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1z3e OCA], [https://pdbe.org/1z3e PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1z3e RCSB], [https://www.ebi.ac.uk/pdbsum/1z3e PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1z3e ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/RPOA_BACSU RPOA_BACSU] DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates.
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/z3/1z3e_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview03.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1z3e ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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Spx, a global transcription regulator in Bacillus subtilis, interacts with the C-terminal domain of the alpha subunit (alphaCTD) of RNA polymerase to control gene expression under conditions of disulfide stress, which is sensed by disulfide bond formation between Spx residues C10 and C13. Here, we describe the crystal structure of the B. subtilis alphaCTD bound to oxidized Spx. Analysis of the complex reveals interactions between three regions of "anti-alpha" Spx and helix alpha1 and the "261" determinant of alphaCTD. The former contact could disrupt the interaction between alphaCTD and activator proteins or alter the DNA-bound conformation of alphaCTD, thereby repressing activator-stimulated transcription. Binding to the 261 determinant would prevent interaction between alphaCTD and region 4 of sigma(A). Intriguingly, the Spx disulfide bond is far from the alphaCTD-Spx interface, suggesting that Spx regulates transcription allosterically or through the redox-dependent creation or destruction of binding sites for additional components of the transcription machinery.
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==About this Structure==
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Crystal structure of the Bacillus subtilis anti-alpha, global transcriptional regulator, Spx, in complex with the alpha C-terminal domain of RNA polymerase.,Newberry KJ, Nakano S, Zuber P, Brennan RG Proc Natl Acad Sci U S A. 2005 Nov 1;102(44):15839-44. Epub 2005 Oct 25. PMID:16249335<ref>PMID:16249335</ref>
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1Z3E is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Bacillus_subtilis Bacillus subtilis] with SO4 as [http://en.wikipedia.org/wiki/ligand ligand]. Active as [http://en.wikipedia.org/wiki/DNA-directed_RNA_polymerase DNA-directed RNA polymerase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.7.6 2.7.7.6] Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1Z3E OCA].
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==Reference==
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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Crystal structure of the Bacillus subtilis anti-alpha, global transcriptional regulator, Spx, in complex with the alpha C-terminal domain of RNA polymerase., Newberry KJ, Nakano S, Zuber P, Brennan RG, Proc Natl Acad Sci U S A. 2005 Nov 1;102(44):15839-44. Epub 2005 Oct 25. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=16249335 16249335]
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</div>
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[[Category: Bacillus subtilis]]
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<div class="pdbe-citations 1z3e" style="background-color:#fffaf0;"></div>
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[[Category: DNA-directed RNA polymerase]]
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[[Category: Protein complex]]
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[[Category: Brennan, R.G.]]
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[[Category: Nakano, S.]]
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[[Category: Newberry, K.J.]]
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[[Category: Zuber, P.]]
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[[Category: SO4]]
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[[Category: bacterial transcription regulation]]
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[[Category: disulfide stress]]
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''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Wed Nov 21 07:13:13 2007''
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==See Also==
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*[[RNA polymerase 3D structures|RNA polymerase 3D structures]]
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== References ==
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<references/>
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__TOC__
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</StructureSection>
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[[Category: Bacillus subtilis]]
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[[Category: Large Structures]]
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[[Category: Brennan RG]]
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[[Category: Nakano S]]
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[[Category: Newberry KJ]]
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[[Category: Zuber P]]

Current revision

Crystal Structure of Spx in Complex with the C-terminal Domain of the RNA Polymerase Alpha Subunit

PDB ID 1z3e

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